Isolation and characterization of diverse nopaline type Ti plasmids of Agrobacterium tumefaciens from Japan

1989 ◽  
Vol 152 (2) ◽  
pp. 119-124 ◽  
Author(s):  
H. Wabiko ◽  
M. Kagaya ◽  
I. Kodama ◽  
K. Masuda ◽  
Y. Kodama ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Isolation And Characterization ◽  
Ti Plasmids

scholarly journals The Presence and Characterization of a virF Gene on Agrobacterium vitis Ti Plasmids

1998 ◽  
Vol 11 (5) ◽  
pp. 429-433 ◽  
Author(s):  
B. Schrammeijer ◽  
J. Hemelaar ◽  
P. J. J. Hooykaas
Keyword(s):  
Amino Acids ◽  
Agrobacterium Tumefaciens ◽  
Molecular Mass ◽  
Promoter Region ◽  
Consensus Sequence ◽  
Tumor Formation ◽  
Nicotiana Glauca ◽  
Agrobacterium Vitis ◽  
Ti Plasmids

Octopine and nopaline strains of Agrobacterium tumefaciens differ in their ability to induce tumors on Nicotiana glauca. The presence of a virF locus on the octopine Ti plasmid makes N. glauca a host plant for these strains, indicating that the VirF protein is a host-range determinant. Here we show the presence of a virF locus not only on the Agrobacterium vitis octopine/cucumopine plasmids pTiAg57 and pTiTm4, but also on the nopaline Ti plas-mids pTiAT1, pTiAT66a, and pTiAT66b. On the octopine Ti plasmids from A. tumefaciens the virF gene is located between the virE locus and the left border of the T-region. In contrast, the virF gene on Ti plasmids of A. vitis is located at the very left end of the vir-region near the virA locus. The virF gene of pTiAg57 has been sequenced and codes for a protein of 202 amino acids with a molecular mass of 22,280 Da. Comparison showed that the virF gene from A. vitis strain Ag57 is almost identical to that from A. tumefaciens octopine strains. The transcription of the pTiAg57 virF is inducible by the plant phenolic compound acetosyringone through the presence of a vir-box consensus sequence in its promoter region. The VirF protein from pTiAg57 can complement octopine A. tumefaciens strains deleted for virF as shown by tumor formation on N. glauca.

scholarly journals Isolation and characterization of a high salt-tolerant and glyphosate-degrading strain of Agrobacterium tumefaciens BZ8

2017 ◽  
Vol 34 (3) ◽  
pp. 739-746
Author(s):  
Shoubao Yan ◽  
Wei Zhao ◽  
Cuie Shi ◽  
Qianqian Tong ◽  
Zhijun Zhai ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
High Salt ◽  
Salt Tolerant ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Agrobacterium tumefaciens Mutants Affected in the Utilization of Octopine, Octopinic Acid and Lysopine

1976 ◽  
Vol 96 (1) ◽  
pp. 155-163 ◽  
Author(s):  
P. M. KLAPWIJK ◽  
P. J. J. HOOYKAAS ◽  
H. C. M. KESTER ◽  
R. A. SCHILPEROORT ◽  
A. RORSCH
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Isolation And Characterization

Isolation and Characterization of a New Virulence Gene (abvA) of Agrobacterium tumefaciens

2001 ◽  
Vol 67 (2) ◽  
pp. 124-133 ◽  
Author(s):  
Parimal MAJUMDER ◽  
Hidenari SHIOIRI ◽  
Masayuki NOZUE ◽  
Mineo KOJIMA
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Virulence Gene ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of behavioural mutants and genes of Agrobacterium tumefaciens

1991 ◽  
Vol 137 (8) ◽  
pp. 1939-1953 ◽  
Author(s):  
C. H. Shaw ◽  
G. J. Loake ◽  
A. P. Brown ◽  
C. S. Garrett ◽  
W. Deakin ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Agrobacterium tumefaciens Strains from Malakander Farm, University of Agriculture, Peshawar

2020 ◽  
Vol 52 (5) ◽  
Author(s):  
Shaheen Rahman ◽  
Kafeel Ahmad ◽  
Niaz Ali ◽  
Muhammad Idrees ◽  
Waqar Ali
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Isolation And Characterization
Sign in / Sign up

Export Citation Format

Share Document