ISOLATION AND CHARACTERIZATION OF FLAVONOID 3' HYDROXYLASE (F3'H) GENE AND GENETIC TRANSFORMATION IN BUTTERFLY PEA (CLITORIA TERNATEA LINN.) VIA AGROBACTERIUM TUMEFACIENS

Department of Genetics, The Hebrew University of Jerusalem. Jerusalem. A variety of mutants which are resistant to triazine - and urea - classes of herbicides have been isolated in the cyanobacterium Synechococcus R2. All the mutants that have been analyzed, show some cross-resistance to different herbicides suggesting that these herbicides share a common binding site in photosystem II. Three psbA genes have been identified in Synechococcus R2. The psbA-copy I gene was cloned from three mutants and used in DNA-mediated genetic transformation. It was found that in all three mutants this gene could transfer the mutation for herbicide resistance indicating that this gene codes for the herbicide resistant protein.

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Isolation and Characterization of Herbicide Resistant Mutants in the Cyanobacterium Synechococcus R2

Zeitschrift für Naturforschung C ◽

10.1515/znc-1987-0619 ◽

1987 ◽

Vol 42 (6) ◽

pp. 758-761 ◽

Cited By ~ 1

Author(s):

Joseph Hirschberg ◽

Nir Ohad ◽

Iris Pecker ◽

Ana Rahat

Keyword(s):

Photosystem Ii ◽

Genetic Transformation ◽

Herbicide Resistance ◽

Cross Resistance ◽

Herbicide Resistant ◽

Isolation And Characterization ◽

Psba Genes ◽

Resistant Mutants ◽

I Gene

A variety of mutants which are resistant to triazine - and urea - classes of herbicides have been isolated in the cyanobacterium Synechococcus R2. All the mutants that have been analyzed, show some cross-resistance to different herbicides suggesting that these herbicides share a common binding site in photosystem II. Three psbA genes have been identified in Synechococcus R2. The psbA-copy I gene was cloned from three mutants and used in DNA-mediated genetic transformation. It was found that in all three mutants this gene could transfer the mutation for herbicide resistance indicating that this gene codes for the herbicide resistant protein.

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Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba

10.21203/rs.2.20139/v2 ◽

2020 ◽

Author(s):

Araceli Oropeza-Aburto ◽

Sergio Alan Cervantes-Perez ◽

Victor A Albert ◽

Luis Rafael Herrera-Estrella

Keyword(s):

Agrobacterium Tumefaciens ◽

Genetic Transformation ◽

In Vitro Selection ◽

Carnivorous Plant ◽

Carnivorous Plants ◽

35S Promoter ◽

Transformation Protocol ◽

Utricularia Gibba

Abstract Background The genus Utricularia belongs to Lentibulariaceae, the largest family of carnivorous plants, which includes terrestrial, epiphytic and aquatic species. The development of specialized structures that evolved for carnivory is a feature of this genus that has been of great interest to biologists since Darwin‘s early studies. Utricularia gibba is itself an aquatic plant with sophisticated bladder traps having one of the most complex suction mechanisms for trapping prey. However, the molecular characterization of the mechanisms that regulate trap development and the biophysical processes involved in prey trapping are still largely unknown due to the lack of a simple and reproducible gene transfer system. Results Here, we report the establishment of a simple, fast and reproducible protocol for genetic transformation of U. gibba based on the T-DNA of Agrobacterium tumefaciens . An in vitro selection system using Phosphinotricin as a selective agent was established for U. gibba . Plant transformation was confirmed by histochemical GUS assays and PCR and qRT-PCR analyses. We report on the expression pattern of the 35S promoter and of the promoter of a trap-specific ribonuclease gene in transgenic U. gibba plants. Conclusions The genetic transformation protocol reported here is an effective method for studying developmental biology and functional genomics of this genus of carnivorous plants and advances the utility of U. gibba as a model system to study developmental processes involved in trap formation.

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