Ultrastructural features of acidic complex carbohydrates in the intercellular matrix of the ovarian follicles in adult mice

1979 ◽  
Vol 60 (2) ◽  
pp. 125-133 ◽  
Author(s):  
Y. Tadano ◽  
K. Yamada
Keyword(s):  
Ovarian Follicles ◽  
Adult Mice ◽  
Intercellular Matrix ◽  
Acidic Complex ◽  
Complex Carbohydrates

The histochemistry of complex carbohydrates in the ovarian follicles of adult mice

1978 ◽  
Vol 57 (3) ◽  
pp. 203-215 ◽  
Author(s):  
Y. Tadano ◽  
K. Yamada
Keyword(s):  
Ovarian Follicles ◽  
Adult Mice ◽  
Complex Carbohydrates

In Vitro Follicle Growth, Maturation, and Gene Expression: Three-Dimensional (3D) Matrigel Culture versus Two-Dimensional Culture (2D)

2021 ◽  
Vol 11 (12) ◽  
pp. 2512-2515
Author(s):  
Byoung-San Moon ◽  
Seungki Lee ◽  
Jung Kyu Choi
Keyword(s):  
Gene Expression ◽  
Three Dimensional ◽  
Ovarian Follicles ◽  
Dimensional System ◽  
Two Dimensional ◽  
Follicle Growth ◽  
Adult Mice ◽  
Three Dimensional Culture

This research aimed to compare the In Vitro growth, maturation, and gene expression in ovarian follicles collected from adult mice (6–8-week-old) between two-dimensional and three-dimensional cultures. First, we confirmed In Vitro follicle growth and maturation using adult mice with outbred characteristics and analyzed the expression of genes related to follicular development. We found that the three-dimensional culture system utilizing a Matrigel drop to create an in vivo-like ovarian microenvironment was more efficient in terms of In Vitro follicle growth, maturation, and gene expression than the two-dimensional system (non-physical environment). The in vivo-like three-dimensional culture of ovarian follicles provides new insights into the physiology and development of ovarian follicle in vivo, thereby contributing to new strategies to improve female fertility.

scholarly journals Pex mRNA Is Localized in Developing Mouse Osteoblasts and Odontoblasts

1998 ◽  
Vol 46 (4) ◽  
pp. 459-468 ◽  
Author(s):  
Andréa Frota Ruchon ◽  
Mieczyslaw Marcinkiewicz ◽  
Géraldine Siegfried ◽  
Harriet S. Tenenhouse ◽  
Luc DesGroseillers ◽  
...  
Keyword(s):  
Mrna Localization ◽  
Northern Analysis ◽  
Phosphate Homeostasis ◽  
Nested Polymerase Chain Reaction ◽  
Newborn Mice ◽  
Matrix Deposition ◽  
Adult Mice ◽  
Intercellular Matrix ◽  
Polymerase Chain

Mutations in PEX, a phosphate-regulating gene with homology to endopeptidase on the X chromosome, were recently identified in patients with X-linked hypophosphatemia (XLH), an inherited disorder of phosphate homeostasis characterized by growth retardation and rachitic and osteomalacic bone disease. To understand the mechanism by which loss of PEX function elicits the mutant phenotype, a study of its mRNA localization and ontogenesis was undertaken. Using the reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) with polyA+ RNA purified from mouse testis, a 337-bp Pex cDNA fragment was generated and cloned in the pCRII plasmid. The cDNA was used to generate sense and anti-sense Pex riboprobes for in situ hybridization (ISH) and Northern analysis. To survey a large number of different tissues, sagittal sections of embryos and newborn mice were examined. ISH showed the presence of Pex mRNA in osteoblasts and odontoblasts. Pex gene expression was detectable on Day 15 of embryonic development, which coincides with the beginning of intercellular matrix deposition in bones. Finally, Northern analysis of total RNA from calvariae and teeth of 3-day-old and adult mice showed that the abundance of the 7-kb Pex transcript is decreased in adult bones and in nongrowing teeth. The present study demonstrates that Pex mRNA is expressed in bones and teeth and suggests that this putative endopeptidase plays an important role in the development of these tissues.

scholarly journals Cytochemical properties of mitochondria in the gastric parietal cell.

1979 ◽  
Vol 27 (4) ◽  
pp. 873-877 ◽  
Author(s):  
P L Sannes ◽  
T Katsuyama ◽  
S S Spicer
Keyword(s):  
Cytochrome Oxidase ◽  
Parietal Cell ◽  
Light And Electron Microscopy ◽  
Intermembrane Space ◽  
Gastric Parietal Cell ◽  
High Iron ◽  
Acidic Complex ◽  
The Matrix ◽  
Complex Carbohydrates ◽  
Gastric Parietal Cells

The matrix of some mitochondria in gastric parietal cells of rat and guinea pig evidenced affinity for the high iron diamine method which localizes sulfated complex carbohydrates selectively by light and electron microscopy. Such staining has not been observed elsewhere in the stomach. The high iron diamine reactive mitochondria about equaled in number those which were unreactive, and the two groups were indistinguishable morphologically. The distinction was not apparent either when mitochondria were stained by other cytochemical procedures including dialyzed iron for acidic complex carbohydrates, 3-3' diaminobenzidine-H2O2 at pH 6.0 for cytochrome oxidase, and Kominick's pyroantimonate osmium tetroxide for antimonate precipitable cations. The dialyzed iron method stained acid glycoconjugates in the outer intermembrane space in parietal cell mitochondria. These mitochondria stained more strongly with dialyzed iron than have any others examined heretofore with this method and comprised the only reactive mitochondria in the stomach. Parietal cell mitochondria also stained strongly for cytochrome oxidase but those of other gastric cells failed to evidence this reactivity.

Binucleate Spermiogenesis in the Mouse

1972 ◽  
Vol 30 ◽  
pp. 112-113
Author(s):  
John J. Wolosewick
Keyword(s):  
Electron Microscopy ◽  
Phosphate Buffer ◽  
Seminiferous Epithelium ◽  
Mouse Testis ◽  
Germinal Epithelium ◽  
Intercellular Bridges ◽  
Adult Mice ◽  
Cervical Dislocation ◽  
Human And Mouse

Classically, the male germinal epithelium is depicted as synchronously developing uninucleate spermatids conjoined by intercellular bridges. Recently, binucleate and multinucleate spermatids from human and mouse testis have been reported. The present paper describes certain developmental events in one type of binucleate spermatid in the seminiferous epithelium of the mouse.Testes of adult mice (ABP Jax) were removed from the animals after cervical dislocation and placed into 2.5% glutaraldehyde/Millonig's phosphate buffer (pH 7.2). Testicular capsules were gently split and separated, exposing the tubules. After 15 minutes the tissue was carefully cut into cubes (approx. 1mm), fixed for an additional 45 minutes and processed for electron microscopy.

Ineffective Thrombopoiesis

1990 ◽  
Vol 48 (3) ◽  
pp. 266-267
Author(s):  
JM Radley ◽  
SL Ellis
Keyword(s):  
Microscopic Examination ◽  
Phosphate Buffer ◽  
Primary Myelofibrosis ◽  
Transient Increase ◽  
Major Difficulty ◽  
Adult Mice ◽  
Dense Cytoplasm

In effective thrombopoies is has been inferred to occur in several disease sates from considerations of megakaryocyte mass and platelet kinetics. Microscopic examination has demonstrated increased numbers of megakaryocytes, with a typical forms particularly pronounced, in primary myelofibrosis. It has not been documented if megakaryocyte ever fail to reach maturity in non-pathological situations. A major difficulty of establishing this is that the number of megakaryocytes normally present in the marrow is extremely low. A large transient increase in megakaryocytopoiesis can how ever be induced in mice by an injection of 5-fluorouracil. We have utilised this treatment and report here evidence for in effective thrombopoies is in healthy mice.Adult mice were perfused (2% glutaraldehyde in 0.08M phosphate buffer, pH 7.4) 8 days following an injection of 5-fluorouracil (150mg/kg). Femurs were subsequently decalcified in 10% neutral E.D.T.A. and embedded in Spurrs resin. Transverse sections of marrow revealed many megakaryocytes at various stages of maturity. Occasional megakaryocytes (less than 1%) were found to be under going degeneration prior to achieving full maturation and releasing cytoplasm as platelets. These cells were characterized by a peripheral rim of dense cytoplasm which enveloped a mass of organelles and vacuoles (Fig. 1). Numerous microtubules were foundaround and with in the organelle-rich zone (Fig 2).

Complex Carbohydrates Part D

1982 ◽  
Keyword(s):  
Part D ◽  
Complex Carbohydrates

PPAR-γ agonist rosiglitazone decreases focal cerebral ischemia-induced inflammation and brain damage in adult mice

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S94-S94
Author(s):  
Kudret Tureyen ◽  
Ramya Sundaresan ◽  
Kellie Bowen ◽  
Raghu Vemuganti
Keyword(s):  
Cerebral Ischemia ◽  
Brain Damage ◽  
Focal Cerebral Ischemia ◽  
Adult Mice ◽  
Ppar Γ
Sign in / Sign up

Export Citation Format

Share Document