Aryl hydrocarbon hydroxylase activity in pulmonary alveolar macrophages and lymphocytes from lung cancer and noncancer patients: A correlation with family histories of cancer

1979 ◽  
Vol 17 (9-10) ◽  
pp. 795-806 ◽  
Author(s):  
T. L. McLemore ◽  
R. R. Martin ◽  
R. R. Springer ◽  
N. Wray ◽  
E. T. Cantrell ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Alveolar Macrophages ◽  
Aryl Hydrocarbon Hydroxylase ◽  
Hydroxylase Activity ◽  
Aryl Hydrocarbon ◽  
Family Histories ◽  
Aryl Hydrocarbon Hydroxylase Activity ◽  
Pulmonary Alveolar Macrophages

The relationship between CYP1A1 aryl hydrocarbon hydroxylase activity and lung cancer in a Japanese population

1998 ◽  
Vol 8 (4) ◽  
pp. 315-323 ◽  
Author(s):  
Chikako Kiyohara ◽  
Yoichi Nakanishi ◽  
Satoru Inutsuka ◽  
Koichi Takayama ◽  
Nobuyuku Hara ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Japanese Population ◽  
Aryl Hydrocarbon Hydroxylase ◽  
Hydroxylase Activity ◽  
Aryl Hydrocarbon ◽  
Aryl Hydrocarbon Hydroxylase Activity ◽  
The Relationship

Aryl-Hydrocarbon Hydroxylase Activity in Lymphocytes from Lung Cancer Patients and Normal Controls

Oncology ◽  
1976 ◽  
Vol 33 (3) ◽  
pp. 105-109 ◽  
Author(s):  
H.A. Guirgis ◽  
H.T. Lynch ◽  
T. Mate ◽  
R.E. Harris ◽  
I. Wells ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Patients ◽  
Aryl Hydrocarbon Hydroxylase ◽  
Hydroxylase Activity ◽  
Lung Cancer Patients ◽  
Aryl Hydrocarbon ◽  
Aryl Hydrocarbon Hydroxylase Activity ◽  
Normal Controls

Measurement of Viability and Aryl Hydrocarbon Hydroxylase Activity in Cryopreserved Baboon Peripheral Blood Lymphocytes and Pulmonary Alveolar Macrophages

1985 ◽  
Vol 4 (2) ◽  
pp. 193-199
Author(s):  
T. L. McLemore ◽  
T. J. Kuehl
Keyword(s):  
Alveolar Macrophages ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blue Dye ◽  
Aryl Hydrocarbon Hydroxylase ◽  
Time Intervals ◽  
Blood Lymphocytes ◽  
Aryl Hydrocarbon ◽  
Aryl Hydrocarbon Hydroxylase Activity ◽  
Pulmonary Alveolar Macrophages

Pulmonary alveolar macrophages (PAMs) and peripheral blood lymphocytes obtained from healthy nonsmoking baboons were evaluated for the ability to retain viability and aryl hydrocarbon hydroxylase (AHH) activity following cryopreservation for 2, 4, 6, 8, and 12 week intervals. Viability was measured (by trypan blue dye exclusion) in lymphocytes following 2, 4, 6, 8, and 12 weeks of cryopreservation. No significant decrease in viability was observed (P > 0.05 for all comparisons; nonpaired 2-tailed, t-test; n = 22), with lymphocytes retaining 88% of their original viability following 12 weeks of cryopreservation. Similarly, a slight, but insignificant decrease in PAM viability was observed following cryopreservation for 2, 4, 6, and 8 week intervals (P > 0.05 in all instances), with PAMs retaining 93.5% of the original 0 time viability. AHH activity was also evaluated in PAMs and lymphocytes following cryopreservation. Lymphocyte noninduced and BA-induced AHH activities were slightly but not significantly decreased after 2, 4, 6, 8, and 12 week cryopreservation intervals (P > 0.05 for all comparisons). Similarly, AHH activity in cultured PAMs was not significantly decreased following cryopreservation for 2, 4, 6, and 8 week intervals (P > 0.05 for all noninduced and BA-induced AHH levels assayed at the various time intervals; n = 8). Even following cryopreservation periods, for 8 or 12 week intervals, PAMs and lymphocytes retained 91% and 90%, respectively, of the original BA-induced AHH activity. These data demonstrate that baboon PAMs and lymphocytes are capable of undergoing cryopreservation while maintaining viability and AHH activity.

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