A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin a and periodic acid-Schiff

1981 ◽  
Vol 71 (4) ◽  
pp. 513-520 ◽  
Author(s):  
K. Yamada ◽  
H. Kitamura ◽  
Y. Maseki
Keyword(s):  
Alkaline Phosphatase ◽  
Concanavalin A ◽  
Staining Method ◽  
Periodic Acid ◽  
Neutral Complex ◽  
Periodic Acid Schiff ◽  
Complex Carbohydrates ◽  
Dual Staining

Concanavalin A-horseradish peroxidase bridge staining of alpha-1 glycoproteins separated by isoelectric focusing on polyacrylamide gel.

1976 ◽  
Vol 24 (8) ◽  
pp. 908-914 ◽  
Author(s):  
R C Allen ◽  
S S Spicer ◽  
D Zehr
Keyword(s):  
Horseradish Peroxidase ◽  
Isoelectric Focusing ◽  
Concanavalin A ◽  
Periodic Acid ◽  
Polyacrylamide Gel ◽  
New Method ◽  
Polyacrylamide Gels ◽  
Periodic Acid Schiff ◽  
Coomassie Blue

The Coomassie Blue protein stain and the periodic acid-Schiff stain for glycoproteins are compared to a new method of staining glycoproteins resolved electrophoretically. The method utilizes a Concanavalin A-horseradish peroxidase sequence to visualize selectively glycoproteins with terminal or internal mannose or terminal N-acetylglucosamine. The method applied to characterization of M and Z allele products of alpha-l-antitrypsins separated by isoelectric focusing of polyacrylamide gels slabs have revealed differences in carbohydrate content of various components that were previously undetected.

A Histochemical Basis for Changes in Rental Tubular Function in Young Mice

1956 ◽  
Vol s3-97 (38) ◽  
pp. 187-195
Author(s):  
JAMES B. LONGLEY ◽  
EDWIN R. FISHER
Keyword(s):  
Alkaline Phosphatase ◽  
Proximal Tubule ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Brush Border ◽  
Morphological Changes ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Pas Reaction ◽  
Young Mice

In the kidney of the adult mouse the tubular epithelium in the most proximal (Pr) segment, corresponding roughly to the convoluted portion of the proximal tubule, shows strong alkaline phosphatase activity, and the brush border is moderately reactive to the periodic acid Schiff (PAS) method. In the more distal (P2) segment of the proximal tubule there is no alkaline phosphatase activity, and the PAS reaction of the brush border is intense. Examination of a large number of young mice has now revealed a distinct pattern in the development of this adult condition. Differentiation of the two segments on the basis of the PAS reaction of the brush border becomes apparent on about the 15th post-natal day. The effect is the result of a decrease in the reactivity of the Pi segment. Differentiation of the two segments on the basis of alkaline phosphatase activity develops gradually between about the 22nd and 36th post-natal days. During this period the alkaline phosphatase activity disappears progressively from the proximal to the distal end of the P2 segment. The administration of testosterone or estradiol to either sex accelerates the differentiation with respect to alkaline phosphatase activity. Castration of male mice retards the completion of this process. It is suggested that morphological changes of this type may provide the basis for some of the functional differences between the kidneys of young and mature animals.

Identification of Glycogen in Thin Sections of Amphibian Embryos

1967 ◽  
Vol 2 (2) ◽  
pp. 257-264
Author(s):  
MARGARET M. PERRY
Keyword(s):  
Staining Method ◽  
Periodic Acid ◽  
Differential Staining ◽  
Small Particles ◽  
Periodic Acid Schiff ◽  
Thin Sections ◽  
Enzymic Digestion ◽  
Cell Structures ◽  
Schiff Reaction ◽  
Glycogen Particles

Embryonic amphibian cells when examined with the electron microscope were observed to contain an abundance of small particles, approximately 325 Å in diameter. The periodic acid/Schiff reaction and enzymic digestion were employed to determine the nature of the particles, and from the results of these tests they were concluded to be glycogen. Treatment of thin sections with periodic acid/lead citrate solutions resulted in a marked increase in contrast of the glycogen particles compared with other cell structures, and in a clearly defined substructure of 40-Å grains appearing within the particles. This differential staining method enabled the particulate glycogen to be distinguished from ribosomes.

Postnatal changes in capillary density of rat sternomastoid muscle

1989 ◽  
Vol 257 (1) ◽  
pp. H344-H347 ◽  
Author(s):  
F. M. Hansen-Smith ◽  
L. Watson ◽  
G. R. Joswiak
Keyword(s):  
Staining Method ◽  
Periodic Acid ◽  
Growth Period ◽  
Capillary Density ◽  
Postnatal Growth ◽  
Periodic Acid Schiff ◽  
Pas Staining ◽  
Old Rats ◽  
Histochemical Marker ◽  
Developing Muscle

The changes in muscle capillarity during postnatal growth have been difficult to study using standard histochemical methods. This laboratory has proposed the use of a lectin, Griffonia simplicifolia I (GSI), as a histochemical marker that may be appropriate for developing muscle. The purpose of the study was to compare the capillary densities (capillaries per muscle fiber) determined by the GSI lectin method, alkaline phosphatase-periodic acid-Schiff (APase-PAS) staining method, and by direct counting from 1-micron plastic sections. Sternomastoid muscles from 10-day or 6-wk-old rats were used. Results from the 10-day rats showed that the GSI method and the 1-micron method gave comparable results (1.44, 1.58 capillaries per fiber), which were significantly higher than that with APase-PAS (0.56). Capillary densities in the white region of the sternomastoid from 6-wk-old rats were identical (2.80) using both the GSI and APase-PAS methods. In contrast, the GSI method yielded significantly higher capillary densities in the red region than did the APase-PAS method (4.80 vs. 3.83). These results indicate that the GSI method for visualizing capillaries is a sensitive method for visualizing capillaries in muscle during the postnatal and juvenile growth period.

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