Natalia Almeida Rodrigues
◽
Claudio Alexandre Gobatto
◽
Lucas Dantas Maia Forte
◽
Filipe Antônio de Barros Sousa
◽
Adriana Souza Torsoni
◽
...
We investigated the effects of the acute and chronic exercise, prescribed in different intensity zones, but with total load-matched on mitochondrial markers (COX-IV, Tfam, and citrate synthase (CS) activity in skeletal muscles, heart, and liver), glycogen stores, aerobic capacity and anaerobic index in swimming rats. For this, two experimental designs were performed (acute and chronic efforts). Load-matched exercises were prescribed below and above and on the anaerobic threshold (AnT), determined by the Lactate Minimum test. In chronic programs, two training prescription strategies were assessed (monotonous and linear periodized model). Results show changes in glycogen stores but no modification in the COX-IV and Tfam contents after acute exercises. In the chronic protocols, COX-IV and Tfam proteins and CS adaptations were intensity and tissue dependents. Monotonous training promoted better adaptations than the periodized model. Training at 80% of the AnT improved both performance variables, emphasizing the anaerobic index, concomitant to CS and COX-IV improvement (soleus muscle). The aerobic capacity and CS activity (gastrocnemius) were increased after 120% AnT training. In conclusion, acute exercise protocol did not promote responses in mitochondrial target proteins. An intensity and tissue dependence are reported in the chronic protocols, highlighting training at 80 and 120% of the AnT.
Novelty:
• Load-matched acute exercise did not enhance COX-IV and Tfam contents in skeletal muscles, heart, and liver.
• In chronic exercise, COX-IV, Tfam, and citrate synthase activity adaptations were intensity and tissue dependents.
•Monotonous training was more efficient than the periodized linear model in adaptations of target proteins and enzymatic activity.