Abstract
Usual bioassay methods fail to establish animal origin of the product. Chemical assay and one-dimensional TLC are satisfactory for detecting adulteration when a single steroid (i.e., hydrocortisone) is substituted in an abnormally high ratio to the total steroids. The problem is more difficult when the adulteration is a sophisticated mixture of steroids in their normal ratios. Two-dimensional TLC of the adrenal cortex injection offers the best evidence of adulteration. The presence or absence of nonsteroidic substances naturally present in animal glands is demonstrable in the chromatograms. A standard of identity of adrenal cortex extract is needed to establish the purity and potency of the injection and its natural origin.
A Comparative Evaluation of Methods for Evolving a Cooperative Team
