Cellular differentiation after free grafting of respiratory epithelia

1991 ◽  
Vol 248 (8) ◽  
pp. 502-507 ◽  
Author(s):  
F. Bootz ◽  
G. Reuter



Author(s):  
Bert Ph. M. Menco ◽  
Ido F. Menco ◽  
Frans L.T. Verdonk

Previously we presented an extensive study of the distributions of intramembranous particles of structures in apical surfaces of nasal olfactory and respiratory epithelia of the Sprague-Dawley rat. For the same structures these distributions were compared in samples which were i) chemically fixed and cryo-protected with glycerol before cryo-fixation, after excision, and ii)ultra-rapidly frozen by means of the slam-freezing method. Since a three-dimensional presentation markedly improves visualization of structural features micrographs were presented as stereopairs. Two exposures were made by tiling the sample stage of the electron microscope 6° in either direction with an eucentric goniometer. The negatives (Agfa Pan 25 Professional) were reversed with Kodak Technical Pan Film 2415 developed in D76 1:1. The prints were made from these reversed negatives. As an example tight-junctional features of an olfactory supporting cell in a region where this cell conjoined with two other cells are presented (Fig. 1).



2005 ◽  
Vol 173 (4S) ◽  
pp. 385-385
Author(s):  
Jason Doles ◽  
Robert Lipinski ◽  
Wade Bushman




Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
E Kisseih Oppong Bekoe ◽  
F Petereit ◽  
C Agyare ◽  
M Lechtenberg ◽  
A Hensel


2013 ◽  
Vol 856 ◽  
pp. 164-168 ◽  
Author(s):  
Diana Irinel Băilă

The behaviour of metallic powders is very important for manufacturing parts and for realize the prototypes. Manufacturing of the orthopaedic implants by selective laser sintering process can be used in medicine. The powders necessary for SLS process are biomaterials who must assure a good osteointegration of endobone implants like the Ti powder (Ti-6Al-4V) and the hydroxyapatite powder. The conditioned powders is a very important process and is necessary in SLS to obtain medical prothesis and influence the final properties of prothesis. Materials must present a gradient for porosity and one for composition (presents of a bioactive phase in titan matrix). The exterior porosity, in the contact zones with the bone tissue, favors the incipient cellular processes (the adhesion and attachment of osteoblastes, proliferation and cellular differentiation and nucleation of mineral bone). The pores must be open and with a specific surface more great. Its preferred the exterior intercommunicating porosity. The presence of phosphates of calcium in the contact zone of the implant with bone favors the mineral kernel on bone. The report Ca/P of phosphates mineral bone has an ascending evolution towards 10/6 according to hydroxyapatite.



2004 ◽  
Vol 43 (5) ◽  
pp. A24-A25
Author(s):  
Jin Chul Paeng ◽  
Dong Soo Lee ◽  
Joo Hyun Kang ◽  
June-Key Chung ◽  
Myung Chul Lee


2021 ◽  
Vol 7 (8) ◽  
pp. eabe3610
Author(s):  
Conor J. Kearney ◽  
Stephin J. Vervoort ◽  
Kelly M. Ramsbottom ◽  
Izabela Todorovski ◽  
Emily J. Lelliott ◽  
...  

Multimodal single-cell RNA sequencing enables the precise mapping of transcriptional and phenotypic features of cellular differentiation states but does not allow for simultaneous integration of critical posttranslational modification data. Here, we describe SUrface-protein Glycan And RNA-seq (SUGAR-seq), a method that enables detection and analysis of N-linked glycosylation, extracellular epitopes, and the transcriptome at the single-cell level. Integrated SUGAR-seq and glycoproteome analysis identified tumor-infiltrating T cells with unique surface glycan properties that report their epigenetic and functional state.



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