Somatic embryogenesis and plant regeneration from cell suspension and tissue cultures of mature himalayan poplar (Populus ciliata)

1989 ◽  
Vol 8 (2) ◽  
pp. 124-127 ◽  
Author(s):  
Gurvinder S. Cheema







1990 ◽  
Vol 32 (1) ◽  
pp. 8-13 ◽  
Author(s):  
Jiřina Duškova ◽  
Z. Opatrný ◽  
Marie Sovová ◽  
J. Dušek


2014 ◽  
Vol 65 (1-2) ◽  
pp. 47-51 ◽  
Author(s):  
Anna Mikuła ◽  
Maria Wesołowska ◽  
Józef Kapusta ◽  
Lutosława Skrzypczak ◽  
Jan J. Rybczyński

The process of plant regeneration via somatic embryogenesis of two gentianas, <em>Gentiana tibetica</em> and <em>G. cruciata</em> was described. For this purpose seedling explants were cultured on agar medium and later maintained in cell suspension. For callus initiation seedling explants like: cotyledons, hypocotyl and root were plated on a callus induction medium (CIM) composed of MS (1962), supplemented with 0.5 mg/l 2,4-D and 1.0 mg/l Kin. For the formation of cell suspension culture, embryogenic callus was transferred into liquid maintained medium (MM) composed of MS (1962), supplemented with 1.0 mg/l Dic + 0.1 mg/l NAA + 2.0 mg/l BAP + 80.0 mg/l SA. The conversion of somatic embryo into plantlets required a new medium (ECM) based on MS (1962) mineral salts, supplemented with 0.5 mg/l GA<sub>3</sub> + 1.0 mg/l Kin + 0.5 mg/l NAA. For cytomorphological studies of particular stages of embryogenesis, specimens were stained with dyes and reagents: 1. PAS reaction with leukofucsin, 2. Safranin + fast green, 3. Erlich's hematoxylin.



1995 ◽  
Vol 14 (10) ◽  
pp. 648-651 ◽  
Author(s):  
Won J. Jeong ◽  
Sung R. Min ◽  
Jang R. Liu


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