Characterization of adenylyl cyclase in heart sarcolemma in the absence or presence of alamethicin

1993 ◽  
Vol 119 (1-2) ◽  
pp. 185-193 ◽  
Author(s):  
Rajat Sethi ◽  
Ken S. Dhalla ◽  
Kanu R. Shah ◽  
Naranjan S. Dhalla
Keyword(s):  
Adenylyl Cyclase ◽  
Heart Sarcolemma

Characterization of Adenylyl Cyclase in Heart Sarcolemma in the Absence or Presence of Alamethicin

1993 ◽  
pp. 185-193
Author(s):  
Rajat Sethi ◽  
Ken S. Dhalla ◽  
Kanu R. Shah ◽  
Naranjan S. Dhalla
Keyword(s):  
Adenylyl Cyclase ◽  
Heart Sarcolemma

scholarly journals Functional Characterization of the Adenylyl Cyclase Genesgs-1by Analysis of a Mutational Spectrum inCaenorhabditis elegans

Genetics ◽  
2002 ◽  
Vol 161 (1) ◽  
pp. 133-142 ◽  
Author(s):  
Celine Moorman ◽  
Ronald H A Plasterk
Keyword(s):  
Life Span ◽  
Adenylyl Cyclase ◽  
Neuronal Degeneration ◽  
Functional Characterization ◽  
Adenylyl Cyclases ◽  
Loss Of Function ◽  
C Elegans ◽  
Larval Stages ◽  
Pharyngeal Pumping

AbstractThe sgs-1 (suppressor of activated Gαs) gene encodes one of the four adenylyl cyclases in the nematode C. elegans and is most similar to mammalian adenylyl cyclase type IX. We isolated a complete loss-of-function mutation in sgs-1 and found it to result in animals with retarded development that arrest in variable larval stages. sgs-1 mutant animals exhibit lethargic movement and pharyngeal pumping and (while not reaching adulthood) have a mean life span that is >50% extended compared to wild type. An extensive set of reduction-of-function mutations in sgs-1 was isolated in a screen for suppressors of a neuronal degeneration phenotype induced by the expression of a constitutively active version of the heterotrimeric Gαs subunit of C. elegans. Although most of these mutations change conserved residues within the catalytic domains of sgs-1, mutations in the less-conserved transmembrane domains are also found. The sgs-1 reduction-of-function mutants are viable and have reduced locomotion rates, but do not show defects in pharyngeal pumping or life span.

scholarly journals Characterization of adenylyl cyclase isoforms in rat peripheral pulmonary arteries

2001 ◽  
Vol 280 (6) ◽  
pp. L1359-L1369 ◽  
Author(s):  
Karen B. Jourdan ◽  
Nicola A. Mason ◽  
Lu Long ◽  
Peter G. Philips ◽  
Martin R. Wilkins ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Adenylyl Cyclase ◽  
Pulmonary Arteries ◽  
Bronchial Epithelium ◽  
Rt Pcr ◽  
Bronchial Smooth Muscle ◽  
Functional Studies ◽  
Kinase C ◽  
Rat Lungs

Activation of adenylyl cyclase (AC), of which there are 10 diversely regulated isoforms, is important in regulating pulmonary vascular tone and remodeling. Immunohistochemistry in rat lungs demonstrated that AC2, AC3, and AC5/6 predominated in vascular and bronchial smooth muscle. Isoforms 1, 4, 7, and 8 localized to the bronchial epithelium. Exposure of animals to hypoxia did not change the pattern of isoform expression. RT-PCR confirmed mRNA expression of AC2, AC3, AC5, and AC6 and demonstrated AC7 and AC8 transcripts in smooth muscle. Western blotting confirmed the presence of AC2, AC3, and AC5/6 proteins. Functional studies provided evidence of cAMP regulation by Ca2+ and protein kinase C-activated but not Gi-inhibited pathways, supporting a role for AC2 and a Ca2+-stimulated isoform, AC8. However, NKH-477, an AC5-selective activator, was more potent than forskolin in elevating cAMP and inhibiting serum-stimulated [3H]thymidine incorporation, supporting the presence of AC5. These studies demonstrate differential expression of AC isoforms in rat lungs and provide evidence that AC2, AC5, and AC8 are functionally important in cAMP regulation and growth pathways in pulmonary artery myocytes.

Characterization of a Ca2+/calmodulin-dependent AC1 adenylyl cyclase in a non-neuronal tissue, the blowfly salivary gland

Cell Calcium ◽  
2012 ◽  
Vol 52 (2) ◽  
pp. 103-112 ◽  
Author(s):  
Kristoffer Heindorff ◽  
Wolfgang Blenau ◽  
Bernd Walz ◽  
Otto Baumann
Keyword(s):  
Salivary Gland ◽  
Adenylyl Cyclase ◽  
Neuronal Tissue

scholarly journals Characterization of adenylyl cyclase stimulated by VIP in rat and mouse peritoneal macrophage membranes

1996 ◽  
Vol 1312 (3) ◽  
pp. 249-254 ◽  
Author(s):  
David Pozo ◽  
J.José Segura ◽  
Isabel Carrero ◽  
Luis G. Guijarro ◽  
Juan C. Prieto ◽  
...  
Keyword(s):  
Adenylyl Cyclase ◽  
Peritoneal Macrophage ◽  
Mouse Peritoneal Macrophage

Molecular Characterization of Adenylyl Cyclase Complex Proteins Using Versatile Protein-Tagging Plasmid Systems in Cryptococcus neoformans

2017 ◽  
Vol 27 (2) ◽  
pp. 357-364 ◽  
Author(s):  
Yee-Seul So ◽  
Dong-Hoon Yang ◽  
Kwang-Woo Jung ◽  
Won-Ki Huh ◽  
Yong-Sun Bahn
Keyword(s):  
Adenylyl Cyclase ◽  
Cryptococcus Neoformans ◽  
Molecular Characterization ◽  
Protein Tagging
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