Concanavalin A receptors and capping in control and activated macrophages

1983 ◽  
Vol 15 (1) ◽  
pp. 59-69 ◽  
Author(s):  
K. Donaldson ◽  
J. M. G. Davis ◽  
K. James
Keyword(s):  
Concanavalin A ◽  
Activated Macrophages ◽  
Concanavalin A Receptors

scholarly journals Modulation of phagosome-lysosome fusion in mouse macrophages.

1981 ◽  
Vol 153 (4) ◽  
pp. 1015-1020 ◽  
Author(s):  
M C Kielian ◽  
Z A Cohn
Keyword(s):  
Concanavalin A ◽  
Immune Serum ◽  
Cross Linking ◽  
Activated Macrophages ◽  
Enzymatic Modification ◽  
Inflammatory Agent ◽  
Proteose Peptone ◽  
Mouse Macrophages ◽  
Control Fusion ◽  
Rate Limiting

A previously described fluorescence assay has been used to characterize factors that modulate phagosome-lysosome (P-L) fusion in mouse macrophages. Fusion was not affected by enzymatic modification or by concanavalin A cross-linking of the plasma membrane or by coating the phagocytic particle with concanavalin A or immune serum. Pretreatment of cells with 10-5-10-4 M colchicine, or treatment immediately after ingestion with 1-10 microgram/ml cytochalasin did not alter P-L fusion; implying that the cytoskeleton does not control fusion in a rate-limiting way. Fusion was strikingly elevated in 5-h cultures of activated macrophages from immune-boosted mice. A lower enhancement was seen in cells activated by proteose-peptone, a nonspecific inflammatory agent.

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