Studies on the bacterial hemoglobin fromVitreoscilla

1991 ◽  
Vol 4 (2) ◽  
pp. 119-125 ◽  
Author(s):  
Peter M. H. Kroneck ◽  
Wolfgang Jakob ◽  
Dale A. Webster ◽  
Richard DeMaio
Keyword(s):  
2006 ◽  
Vol 9 (11) ◽  
pp. 2022-2031 ◽  
Author(s):  
Khaled M. Khleifa ◽  
Muayad Abboud . ◽  
Mohammad Laymun . ◽  
Khalid Al-Sharafa . ◽  
Khaled Tarawneh .

1992 ◽  
Vol 293 (2) ◽  
pp. 241-245 ◽  
Author(s):  
Rajendra P. Dikshit ◽  
Kanak L. Dikshit ◽  
Yixiang Liu ◽  
Dale A. Webster

2013 ◽  
Vol 195 (13) ◽  
pp. 3115-3123 ◽  
Author(s):  
W. G. Fusco ◽  
N. R. Choudhary ◽  
S. E. Council ◽  
E. J. Collins ◽  
I. Leduc

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Aleksandra M. Mirończuk ◽  
Katarzyna E. Kosiorowska ◽  
Anna Biegalska ◽  
Magdalena Rakicka-Pustułka ◽  
Mateusz Szczepańczyk ◽  
...  

Abstract Background Yarrowia lipolytica is an unconventional yeast with a huge industrial potential. Despite many advantages for biotechnological applications, it possesses enormous demand for oxygen, which is a bottleneck in large scale production. In this study a codon optimized bacterial hemoglobin from Vitreoscilla stercoraria (VHb) was overexpressed in Y. lipolytica for efficient growth and erythritol synthesis from glycerol in low-oxygen conditions. Erythritol is a natural sweetener produced by Y. lipolytica under high osmotic pressure and at low pH, and this process requires high oxygen demand. Results Under these conditions the VHb overexpressing strain showed mostly yeast-type cells resulting in 83% higher erythritol titer in shake-flask experiments. During a bioreactor study the engineered strain showed higher erythritol productivity (QERY = 0.38 g/l h) and yield (YERY = 0.37 g/g) in comparison to the control strain (QERY = 0.30 g/l h, YERY = 0.29 g/g). Moreover, low stirring during the fermentation process resulted in modest foam formation. Conclusions This study showed that overexpression of VHb in Y. lipolytica allows for dynamic growth and efficient production of a value-added product from a low-value substrate.


FEBS Letters ◽  
2011 ◽  
Vol 585 (20) ◽  
pp. 3250-3258 ◽  
Author(s):  
Aik-Hong Teh ◽  
Jennifer A. Saito ◽  
Aida Baharuddin ◽  
Jason R. Tuckerman ◽  
James S. Newhouse ◽  
...  
Keyword(s):  

Author(s):  
Cataldo Tarricone ◽  
Sabina Calogero ◽  
Alessandro Galizzi ◽  
Alessandro Coda ◽  
Paolo Ascenzi ◽  
...  

1998 ◽  
Vol 349 (1) ◽  
pp. 161-166 ◽  
Author(s):  
Kanak L. Dikshit ◽  
Yutaka Orii ◽  
Naveen Navani ◽  
Sangeeta Patel ◽  
Hui-Yu Huang ◽  
...  

1993 ◽  
Vol 11 (8) ◽  
pp. 926-929 ◽  
Author(s):  
J. A. DeModena ◽  
S. Gutiérrez ◽  
J. Velasco ◽  
F. J. Fernández ◽  
R. A. Fachini ◽  
...  

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