Effect of cerebeal neurite-stimulating protein on morphogenesis of organotypical culture of spinal ganglia

1989 ◽  
Vol 19 (2) ◽  
pp. 124-129
Author(s):  
V. P. Goncharova ◽  
O. S. Sotnikov ◽  
A. V. Romanyuk ◽  
N. I. Chalisova ◽  
E. I. Chumasov
Keyword(s):  
Spinal Ganglia ◽  
Organotypical Culture

Survival and phenotypic characteristics of axotomized neurons in spinal ganglia

2005 ◽  
Vol 35 (5) ◽  
pp. 457-460 ◽  
Author(s):  
Yu. A. Chelyshev ◽  
I. S. Raginov ◽  
D. S. Guseva ◽  
R. F. Masgutov
Keyword(s):  
Spinal Ganglia ◽  
Phenotypic Characteristics

scholarly journals STUDIES OF THE DEGENERATION AND REGENERATION OF AXIS CYLINDERS IN VITRO

1913 ◽  
Vol 17 (2) ◽  
pp. 182-191 ◽  
Author(s):  
Ragnvald Ingebrigtsen
Keyword(s):  
Spinal Cord ◽  
Degenerative Changes ◽  
Spinal Ganglia ◽  
Chick Embryos

1. The brains of chick embryos, of cats six weeks old, of rabbits two months old, and of dogs three weeks old, when cultivated in vitro, develop long filaments which, according to their growth and their anatomical and tinctorial characters, must be considered as true axis cylinders. 2. Similar structures develop from spinal ganglia of rabbits seven months old, and from the spinal cord of cats six weeks old, and of rabbits two months old. 3. When severed from their origin by section these threads undergo degenerative changes which do not appear after nine hours, but which are seen after twenty hours, and continue until in the course of the following two days the thread degenerates completely. 4. After twenty hours the development of new axis cylinders from the central part of the cut fibers is observed.

The in vitro effect of the nerve growth factor on chick embryo spinal ganglia—a light-microscopic evaluation

Development ◽  
1970 ◽  
Vol 24 (2) ◽  
pp. 381-392
Author(s):  
Peddrick Weis
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Chick Embryo ◽  
Culture Period ◽  
Spinal Ganglia ◽  
Nerve Growth ◽  
Microscopic Evaluation ◽  
Vitro Effect

The effect of the nerve growth factor (NGF) on chick embryo spinal ganglia was studied in the hanging-drop bioassay system by comparison with parallel development in vivo. The well-differentiated ventrolateral neuroblasts, which in vivo increase 1·33 times in size during the culture period, did not increase in size at all in vitro. Only 65–72% survived to the end of the culture period regardless of the NGF concentration. The less-differentiated mediodorsal (M-D) neuroblasts, which in vivo increase 1·31 times in size during the culture period, were found to increase equally in vitro if sufficient NGF was present. Such a quantity was greater than that which evoked maximum outgrowth of neurites. Survival of M-D neuroblasts was also related to NGF concentration but did not equal the in vivo condition even at the highest concentration. The hyperchromatic type of degeneration prevented by high NGF concentrations is that which results in vivo from insufficient peripheral field. From this and other reports it would appear that the response to NGF seen in vitro is due only to the M-D neuroblasts, and that all biochemical and cytological observations which have been reported would therefore represent conditions within those cells only.

Cell turnover in the spinal ganglia of Xenopus laevis tadpoles

Development ◽  
1965 ◽  
Vol 13 (1) ◽  
pp. 63-72
Author(s):  
M. C. Prestige
Keyword(s):  
Balance Sheet ◽  
Ventral Horn ◽  
Spinal Ganglia ◽  
Sensory Cells ◽  
Cell Turnover ◽  
Hind Limbs ◽  
Time Graph ◽  
Relationship Of ◽  
The Relationship ◽  
Developing Spinal Cord

Hughes (1961) has drawn up a balance-sheet of the lumbar ventral horn cells during the development of Xenopus, based both on counts of numbers of ventral horn cells throughout differentiation and of the number of degenerating cells among them at each stage. In this way, he showed that the total number of ventral horn cells is reduced from between 3000 and 4000 at differentiation to 1200 at the end of metamorphosis; that the most rapid period of decline in cell numbers is accompanied by a peak in the time graph of the number of degenerations; and concluded that for every neurone which finally differentiates, some eight or nine neuroblasts undergo degeneration. This is an example of histogenetic degeneration (Glücksmann, 1951), that is, cell death relating to the differentiation of functioning organs. In order to investigate further the relationship of the limb to the developing spinal cord, a similar balance-sheet has been drawn up for the sensory cells of the hind-limbs which are in dorsal ganglia 8–10

Sign in / Sign up

Export Citation Format

Share Document