Study of colour-developing factors in spectrophotometric determination of cyanide by the pyridine-barbituric acid method

1980 ◽  
Vol 74 (1-2) ◽  
pp. 9-16 ◽  
Author(s):  
Chun -Ching Chien ◽  
Fu -Chung Chang ◽  
Shaw -Chii Wu
Keyword(s):  
Spectrophotometric Determination ◽  
Barbituric Acid ◽  
Acid Method

Spectrophotometric Determination of Barbituric Acid

1997 ◽  
Vol 30 (15) ◽  
pp. 2765-2777 ◽  
Author(s):  
Tehseen Aman ◽  
Islam-Ullah Khan ◽  
Zahida Parveen
Keyword(s):  
Spectrophotometric Determination ◽  
Barbituric Acid

Spectrophotometric Determination of Cyanoglucosides in Cassava

1984 ◽  
Vol 67 (3) ◽  
pp. 641-643 ◽  
Author(s):  
Bala Nambisan ◽  
Sukumaran Sundaresan
Keyword(s):  
Detection Limit ◽  
Sodium Hydroxide ◽  
Spectrophotometric Determination ◽  
Phosphate Buffer ◽  
Barbituric Acid ◽  
New Method ◽  
Minimum Detection Limit ◽  
Rapid Inactivation ◽  
Chloramine T

Abstract A new method is reported for determination of cyanoglucosides in cassava. The method is simple, rapid, and sensitive. Ten g cassava tuber is homogenized with warm (65–70°C) 80% ethanol (1 + 6, w/v) to extract cyanoglucosides (CNG). The ethanol is evaporated, and an aliquot of the extract (0.1–0.2 mL) is incubated with added linamarase in pH 6.0 phosphate buffer for 15 min at 30°C. The reaction is stopped by adding 0.2N sodium hydroxide, the solution is neutralized, and cyanide is estimated by adding chloramine T and barbituric acid–pyridine reagent and measuring the absorbance at 570 nm. Complete CNG extraction and rapid inactivation of endogenous linamarase is possible with 80% ethanol. There is no interference from extractives in the linamarase reaction or in the estimation of cyanide. Recovery of added linamarin (as cyanide) is 98% by this assay. The minimum detection limit of cyanide in the assay is 0.1 μg/mL.

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