New method for spectrophotometric determination of quinones and barbituric acid through their reaction. A kinetic study

1996 ◽  
Vol 52 (12) ◽  
pp. 1679-1684 ◽  
Author(s):  
H.A.A. Medien
Keyword(s):  
Kinetic Study ◽  
Spectrophotometric Determination ◽  
Barbituric Acid ◽  
New Method

Spectrophotometric Determination of Cyanoglucosides in Cassava

1984 ◽  
Vol 67 (3) ◽  
pp. 641-643 ◽  
Author(s):  
Bala Nambisan ◽  
Sukumaran Sundaresan
Keyword(s):  
Detection Limit ◽  
Sodium Hydroxide ◽  
Spectrophotometric Determination ◽  
Phosphate Buffer ◽  
Barbituric Acid ◽  
New Method ◽  
Minimum Detection Limit ◽  
Rapid Inactivation ◽  
Chloramine T

Abstract A new method is reported for determination of cyanoglucosides in cassava. The method is simple, rapid, and sensitive. Ten g cassava tuber is homogenized with warm (65–70°C) 80% ethanol (1 + 6, w/v) to extract cyanoglucosides (CNG). The ethanol is evaporated, and an aliquot of the extract (0.1–0.2 mL) is incubated with added linamarase in pH 6.0 phosphate buffer for 15 min at 30°C. The reaction is stopped by adding 0.2N sodium hydroxide, the solution is neutralized, and cyanide is estimated by adding chloramine T and barbituric acid–pyridine reagent and measuring the absorbance at 570 nm. Complete CNG extraction and rapid inactivation of endogenous linamarase is possible with 80% ethanol. There is no interference from extractives in the linamarase reaction or in the estimation of cyanide. Recovery of added linamarin (as cyanide) is 98% by this assay. The minimum detection limit of cyanide in the assay is 0.1 μg/mL.

Benzyl ethers of methyl α-D-glucopyranoside

1980 ◽  
Vol 45 (7) ◽  
pp. 1959-1963 ◽  
Author(s):  
Dušan Joniak ◽  
Božena Košíková ◽  
Ludmila Kosáková
Keyword(s):  
Kinetic Study ◽  
Spectrophotometric Determination ◽  
Reductive Cleavage ◽  
Ether Bond ◽  
Benzyl Ethers ◽  
Acid Catalyzed ◽  
Model Substances ◽  
Hydrolysis Of

Methyl 4-O-(3-methoxy-4-hydroxybenzyl) and methyl 4-O-(3,5-dimethoxy-4-hydroxybenzyl)-α-D-glucopyranoside and their 6-O-isomers were prepared as model substances for the ether lignin-saccharide bond by reductive cleavage of corresponding 4,6-O-benzylidene derivatives. Kinetic study of acid-catalyzed hydrolysis of the compounds prepared was carried out by spectrophotometric determination of the benzyl alcoholic groups set free, after their reaction with quinonemonochloroimide, and it showed the low stability of the p-hydroxybenzyl ether bond.

A new method for spectrophotometric determination of thiomersal in biologicals

Biologicals ◽  
1995 ◽  
Vol 23 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Keshaw Prasad Shrivastaw ◽  
Sarbjit Singh
Keyword(s):  
Spectrophotometric Determination ◽  
New Method

scholarly journals A new method for the spectrophotometric determination of aliphatic amines with a "crowned" dinitrophenylazophenol-Ba(II) complex.

1984 ◽  
Vol 32 (6) ◽  
pp. 2468-2470 ◽  
Author(s):  
Kenichiro Nakashima ◽  
Noriko Mochizuki ◽  
Shinichi Nakatsuji ◽  
Shuzo Akiyama ◽  
Takahiro Kaneda ◽  
...  
Keyword(s):  
Spectrophotometric Determination ◽  
New Method ◽  
Aliphatic Amines
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