Nucleotide sequence comparisons of turnip yellow mosaic virus isolates from Australia and Europe

1987 ◽  
Vol 97 (3-4) ◽  
pp. 283-295 ◽  
Author(s):  
J. Blok ◽  
A. Mackenzie ◽  
P. Guy ◽  
A. Gibbs
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
Sequence Comparisons ◽  
Virus Isolates

scholarly journals Turnip yellow mosaic virus isolates with experimentally produced recombinant virion proteins.

1998 ◽  
Vol 79 (2) ◽  
pp. 395-403 ◽  
Author(s):  
C M Hayden ◽  
M L Skotnicki ◽  
A M Mackenzie ◽  
A Gibbs
Keyword(s):  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
Virus Isolates

scholarly journals Proteolytic Processing of Turnip Yellow Mosaic Virus Replication Proteins and Functional Impact on Infectivity

2007 ◽  
Vol 81 (20) ◽  
pp. 11402-11412 ◽  
Author(s):  
Anna Jakubiec ◽  
Gabrièle Drugeon ◽  
Laurent Camborde ◽  
Isabelle Jupin
Keyword(s):  
Mosaic Virus ◽  
Rna Virus ◽  
Proteolytic Processing ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
Replication Proteins ◽  
Sequence Comparisons ◽  
Positive Strand ◽  
Positive Strand Rna

ABSTRACT Turnip yellow mosaic virus (TYMV), a positive-strand RNA virus belonging to the alphavirus-like supergroup, encodes its nonstructural replication proteins as a 206K precursor with domains indicative of methyltransferase (MT), proteinase (PRO), NTPase/helicase (HEL), and polymerase (POL) activities. Subsequent processing of 206K generates a 66K protein encompassing the POL domain and uncharacterized 115K and 85K proteins. Here, we demonstrate that TYMV proteinase mediates an additional cleavage between the PRO and HEL domains of the polyprotein, generating the 115K protein and a 42K protein encompassing the HEL domain that can be detected in plant cells using a specific antiserum. Deletion and substitution mutagenesis experiments and sequence comparisons indicate that the scissile bond is located between residues Ser879 and Gln880. The 85K protein is generated by a host proteinase and is likely to result from nonspecific proteolytic degradation occurring during protein sample extraction or analysis. We also report that TYMV proteinase has the ability to process substrates in trans in vivo. Finally, we examined the processing of the 206K protein containing native, mutated, or shuffled cleavage sites and analyzed the effects of cleavage mutations on viral infectivity and RNA synthesis by performing reverse-genetics experiments. We present evidence that PRO/HEL cleavage is critical for productive virus infection and that the impaired infectivity of PRO/HEL cleavage mutants is due mainly to defective synthesis of positive-strand RNA.

scholarly journals Molecular characterisation of Zucchini yellow mosaic virus infecting Cucurbita pepo in Egypt

2021 ◽  
Author(s):  
Engy Abdel Aleem ◽  
Mona Rabie ◽  
Faiza Fattouh
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Complete Nucleotide Sequence ◽  
Zucchini Yellow Mosaic Virus ◽  
First Record ◽  
Yellow Mosaic Virus ◽  
Sequence Comparisons ◽  
The Usa ◽  
Group A ◽  
Egyptian Isolate

The complete nucleotide sequence of Zucchini yellow mosaic virus isolate from Egypt (ZYMV-Egz_MT383108) was determined. The sequence comparisons suggested that the isolate belongs to Group A. The sequence analysis of the Egyptian isolate showed the highest similarity (~96–97%) with the isolates leaf1 (KJ923767.1) and PA_2006 (JQ716413.1) from the USA and the lowest similarity (84%) with an isolate (AF014811.2) from Singapore. The phylogenetic analysis revealed that ZYMV-Egz occupied a distinct clade together with the USA isolates in Group A, known to be the most widespread throughout the world. This is a first record of the complete nucleotide sequence of an Egyptian isolate of ZYMV.

Organization of the Genome of Turnip Yellow Mosaic Virus deduced from its Messenger Properties

1979 ◽  
Vol 7 (5) ◽  
pp. 1095-1097 ◽  
Author(s):  
CLAUDE BÉNICOURT ◽  
MARIE-DOMINIQUE MORCH
Keyword(s):  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus

Small-angle X-ray scattering from turnip yellow mosaic virus

1954 ◽  
Vol 14 ◽  
pp. 1-11 ◽  
Author(s):  
Paul Schmidt ◽  
Paul Kaesberg ◽  
W.W. Beeman
Keyword(s):  
Mosaic Virus ◽  
Small Angle ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
X Ray ◽  
X Ray Scattering ◽  
Ray Scattering
Sign in / Sign up

Export Citation Format

Share Document