scholarly journals Molecular characterisation of Zucchini yellow mosaic virus infecting Cucurbita pepo in Egypt

2021 ◽  
Author(s):  
Engy Abdel Aleem ◽  
Mona Rabie ◽  
Faiza Fattouh
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Complete Nucleotide Sequence ◽  
Zucchini Yellow Mosaic Virus ◽  
First Record ◽  
Yellow Mosaic Virus ◽  
Sequence Comparisons ◽  
The Usa ◽  
Group A ◽  
Egyptian Isolate

The complete nucleotide sequence of Zucchini yellow mosaic virus isolate from Egypt (ZYMV-Egz_MT383108) was determined. The sequence comparisons suggested that the isolate belongs to Group A. The sequence analysis of the Egyptian isolate showed the highest similarity (~96–97%) with the isolates leaf1 (KJ923767.1) and PA_2006 (JQ716413.1) from the USA and the lowest similarity (84%) with an isolate (AF014811.2) from Singapore. The phylogenetic analysis revealed that ZYMV-Egz occupied a distinct clade together with the USA isolates in Group A, known to be the most widespread throughout the world. This is a first record of the complete nucleotide sequence of an Egyptian isolate of ZYMV.

scholarly journals The Complete Nucleotide Sequence of Bean Yellow Mosaic Virus Genomic RNA.

1996 ◽  
Vol 62 (5) ◽  
pp. 472-477 ◽  
Author(s):  
Shigeo NAKAMURA ◽  
Ryoso HONKURA ◽  
Takayoshi IWAI ◽  
Masashi UGAKI ◽  
Yuko OHASHI
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Complete Nucleotide Sequence ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Genomic Rna

scholarly journals Increase in Zucchini yellow mosaic virus Symptom Severity in Tolerant Zucchini Cultivars Is Related to a Point Mutation in P3 Protein and Is Associated with a Loss of Relative Fitness on Susceptible Plants

2003 ◽  
Vol 93 (12) ◽  
pp. 1478-1484 ◽  
Author(s):  
C. Desbiez ◽  
A. Gal-On ◽  
M. Girard ◽  
C. Wipf-Scheibel ◽  
H. Lecoq
Keyword(s):  
Mosaic Virus ◽  
Point Mutation ◽  
Zucchini Yellow Mosaic Virus ◽  
Genetic Load ◽  
Yellow Mosaic Virus ◽  
Relative Fitness ◽  
Interspecific Crosses ◽  
Mixed Infections ◽  
Wild Type ◽  
Sequence Comparisons

Zucchini yellow mosaic virus (ZYMV, Potyvirus) is a very damaging cucurbit virus worldwide. Interspecific crosses with resistant Cucurbita moschata have led to the release of “resistant” zucchini squash (C. pepo) F1 hybrids. However, although the resistance is almost complete in C. moschata, the commercial C. pepo hybrids are only tolerant. ZYMV evolution toward increased aggressiveness on tolerant hybrids was observed in the field and was obtained experimentally. Sequence comparisons and recombination experiments revealed that a point mutation in the P3 protein of ZYMV was enough to induce tolerance breaking. Competition experiments were performed between quasi-isogenic wild-type, and aggressive variants of ZYMV distinguished by monoclonal antibodies. The aggressive mutants were more fit than wild-type strains in mixed infections of tolerant zucchini, but they presented a drastic fitness loss in mixed infections of susceptible zucchini or melon. Thus, the ability to induce severe symptoms in tolerant zucchini is related to a genetic load in susceptible zucchini, but also on other susceptible hosts. This represents the first quantitative study of the fitness cost associated with tolerance breaking for a plant virus. Thus, although easily broken, the tolerance might prove durable in some conditions if the aggressive variants are counterselected in susceptible crops.

Complete nucleotide sequence of rose yellow mosaic virus, a novel member of the family Potyviridae

2013 ◽  
Vol 158 (9) ◽  
pp. 1917-1923 ◽  
Author(s):  
Dimitre Mollov ◽  
Ben Lockhart ◽  
David Zlesak
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Complete Nucleotide Sequence ◽  
Yellow Mosaic Virus ◽  
The Family

scholarly journals Isolation and Molecular Characterization of a Chilean Isolate of Zucchini yellow mosaic virus

Plant Disease ◽  
2001 ◽  
Vol 85 (6) ◽  
pp. 644-648 ◽  
Author(s):  
H. Prieto ◽  
A. Bruna ◽  
P. Hinrichsen ◽  
C. Muñoz
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sequence Comparisons ◽  
Severe Stunting ◽  
Chilean Isolate

Zucchini yellow mosaic virus (ZYMV) was described in 1981 affecting squash, melon, and other cultivated cucurbits with severe stunting and yellowing symptoms. It was reported to be present in most countries where cucurbits are grown, and in Chile since 1995, from surveys using enzyme-linked immunosorbent assay (ELISA) but without further characterization. A potyvirus was isolated from ELISA-positive symptomatic plants. The results indicate that this virus is ZYMV based on symptoms on herbaceous indicators, immunospecific electron microscopy of the purified particle, and sequencing of 395 bases of the 3′ end of the coat protein gene. The virus was detected in melon, watermelon, and squash plants. In agreement with previous descriptions for ZYMV, the Chilean isolate is a flexuous filamentous particle 740 nm long with one main protein of approximately 36 kDa. Nucleotide sequence comparisons of the 3′ portion of the coat protein gene revealed a high similarity to the Connecticut and California strains.

scholarly journals First Report of Squash leaf curl Philippines virus Infecting Chayote (Sechium edule) in Taiwan

Plant Disease ◽  
2011 ◽  
Vol 95 (9) ◽  
pp. 1197-1197 ◽  
Author(s):  
W. S. Tsai ◽  
C. J. Hu ◽  
D. P. Shung ◽  
L. M. Lee ◽  
J. T. Wang ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Nucleotide Sequence Identity ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
First Report ◽  
Sequence Identity ◽  
Sechium Edule ◽  
Complementary Sense ◽  
Leaf Curl

Young shoots and leaves of chayote (Sechium edule (Jacq.) Sw.) are commonly consumed as a vegetable in Taiwan. In Hualien County, the major chayote-production area of Taiwan, as much as 15% of chayote plants were not marketable between September and October 2010 because of mosaic symptoms on the leaves. Three symptomatic leaves were collected from each of three fields in Hualien. All nine samples tested positive for a begomovirus by PCR using general primer pair PAL1v1978B/PAR1c715H (3) and negative for Zucchini yellow mosaic virus, Cucumber mosaic virus, Cucumber green mottle mosaic virus, Melon yellow spot virus, Papaya ringspot virus - type W, Watermelon mosaic virus, and Watermelon silver mottle virus by ELISA (2). On the basis of the high nucleotide sequence identity (97.7 to 99.6%) of the 1.5-kb begomoviral DNA-A fragments, all nine samples were considered infected by the same begomovirus species. The 1.5-kb sequences had greatest nucleotide sequence identity (96.6 to 97.8%) with Squash leaf curl Philippines virus (SLCPHV) pumpkin isolate from Taiwan (1) (GenBank Accession No. DQ866135; SLCPHV-TW[TW:Pum:05]). One sample was selected to complete viral genomic DNA analysis. Abutting primer pairs PKA-V/C (PKA-V: 5′-AACGGATCCACTTATGCACGATTTCCCT-3′; PKA-C: 5′-TAAGGATCCCACATGTTGTGGAGCA-3′) and PKB-V/C (PKB-V: 5′-TGTCCATGGATTGATGCGTTATCGGA-3′; PKB-C: 5′-TGACCATGGCATTTCCGAGATCTCCCA-3′') were used to amplify the complete DNA-A and DNA-B, respectively. The sequences of DNA-A (GenBank Accession No. JF146795) and DNA-B (GenBank Accession No. JF146796) contain 2,734 and 2,715 nucleotides, respectively. The geminivirus conserved sequence TAATATTAC was found in both DNA-A and -B. The DNA-A has two open reading frames (ORFs) in the virus sense (V1 and V2) and four in the complementary sense (C1 to C4). The DNA-B also had one ORF each in the virus sense (BV1) and the complementary sense (BC1). When compared by BLASTn in GenBank and analyzed by MEGALIGN software (DNASTAR, Madison, WI), they were found to have greatest nucleotide identity (98.0 to 99.0% of DNA-A and 96.7% of DNA-B) with SLCPHV isolates from Taiwan. In addition, SLCPHV caused similar symptoms on leaves when transmitted to healthy chayote by viruliferous whitefly. In Taiwan, SLCPHV has been detected and sequenced from naturally infected melon (GenBank Accession No. EU479710), pumpkin (GenBank Accession No. DQ866135), and wax gourd (GenBank Accession No. EU310406). To our knowledge, this is the first report of SLCPHV infecting chayote plants in Taiwan. The prevalence of SLCPHV infection on different cucurbit crops should be taken into consideration for managing viral diseases in Taiwan. References: (1) W. S. Tsai et al. Plant Dis. 91:907, 2007. (2) W. S. Tsai et al. Plant Dis. 94:923, 2010. (3) W. S. Tsai et al. Online publication. doi: 10.1111/j.1365-3059.2011.02424.x. Plant Pathol., 2011.

scholarly journals Phylogenetic analysis of see-transmitted isolate of Zucchini yellow mosaic virus

2017 ◽  
Vol 74 (2) ◽  
pp. 46-50
Author(s):  
O. Tymchyshyn ◽  
I. Kosenko ◽  
T. Shevchenko Shevchenko ◽  
O. Shevchenko ◽  
I. Budzanivska
Keyword(s):  
Phylogenetic Analysis ◽  
Mosaic Virus ◽  
Cucurbita Pepo ◽  
Transmission Rate ◽  
Zucchini Yellow Mosaic Virus ◽  
Seed Transmission ◽  
Yellow Mosaic Virus ◽  
Rt Pcr ◽  
Group A ◽  
Cucurbitaceae Family

Zucchini yellow mosaic virus (ZYMV) remains one of the most widespread and destructive viruses affecting plants from Cucurbitaceae family in Ukraine as well as in other countries. ZYMV during the early stages of plant development can cause significant losses in yield. In current project the possibility of seed transmission of Ukrainian ZYMV isolates was tested on Cucurbita pepo plants in insect-free greenhouse. The rate was assessed by ELISA and RT-PCR. Only one isolate ZYMV-14P showed seedborne transmission with transmission rate 2,6%. This is the first detected seed-transmitted isolate in Ukraine. Phylogenetic analysis defined ZYMV-14P isolate as member of group A. This isolate was clustered with other known Ukrainian isolates and isolates from Hungary, Czech Republic, Austria and France within subgroup AI.

scholarly journals First Report of Zucchini yellow mosaic virus in Cucurbits in Ivory Coast

Plant Disease ◽  
2010 ◽  
Vol 94 (11) ◽  
pp. 1378-1378 ◽  
Author(s):  
D. Koné ◽  
S. Aké ◽  
K. Abo ◽  
S. Soro ◽  
C. A. N'Guessan ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Ivory Coast ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
African Countries ◽  
Cucumis Sativus L ◽  
First Report ◽  
Phylogenetic Cluster ◽  
Group A

During a field survey conducted in December 2008 and January 2009 in southern Ivory Coast, zucchini squash (Cucurbita pepo L.) and cucumber (Cucumis sativus L.) plants were observed showing severe symptoms of leaf mosaic and distortions, filiformism, and fruit deformations. Nine samples were collected from symptomatic plants in four locations (Adzopé, Songon, Ayamé, and Gagnoa) and dried over CaCl2. Double-antibody sandwich (DAS)-ELISA tests were performed directly on dried samples with antisera against nine cucurbit-infecting viruses: Zucchini yellow mosaic virus (ZYMV, Potyvirus); Papaya ringspot virus (PRSV, Potyvirus); Watermelon mosaic virus (WMV, Potyvirus); Moroccan watermelon mosaic virus (MWMV, Potyvirus); Cucumber vein yellowing virus (CVYV, Ipomovirus); Cucumber mosaic virus (CMV, Cucumovirus); Cucurbit aphid borne yellows virus (CABYV, Polerovirus); Squash mosaic virus (SqMV, Comovirus); and Cucumber green mottle mosaic virus (CGMMV, Tobamovirus). ZYMV was detected alone in four of six zucchini squash samples and in mixed infection with CMV and PRSV in two of three cucumber samples. A cucumber sample (CI09-09) collected at Songon and infected by ZYMV, CMV, and PRSV was inoculated to zucchini squash. ZYMV was separated from CMV and PRSV by inoculating zucchini squash plantlets with one Myzus persicae Sulzer per plant with 2-min acquisition and 2-h inoculation access periods. Plants infected by ZYMV only developed typical symptoms of severe mosaic, distortion, and filiformism on leaves. Total RNA was extracted from the original dried sample of CI09-09 using TRI-Reagent (Molecular Research Center Inc., Cincinnati, OH) (2). One-step reverse transcription (RT)-PCR was performed with our standard protocol and specific primers (2), yielding a 605-bp fragment corresponding to part of the polymerase (NIb) and coat protein (CP) coding regions. The nucleotide sequence of the NIb-CP fragment of Ivory Coast ZYMV isolate CI09-09 (GenBank No. HM450303) shared 98.5, 92.7, 80.5, and 75.7% identity with ZYMV isolates from France (isolate E9, HM641798), Florida (D13914), Singapore (AF014811), and Vietnam (DQ925449), respectively. Sequence comparison indicated that CI09-09 belongs to the phylogenetic cluster 1 of group A of ZYMV (2). ZYMV, first described in 1981, is now one of the most damaging viruses in cucurbit crops worldwide and is characterized by an important biological and molecular diversity (1,3). ZYMV has already been reported in several African countries, mostly in the northern and southern parts of the continent (1), but to our knowledge, this is the first report of ZYMV in Ivory Coast. Among African isolates, CI09-09 shared 97.5% identity with isolate Su06-22 from Sudan (HM641799) belonging to the phylogenetic cluster 1 of group A of ZYMV, 94 to 95% identity with isolates from neighboring Mali (HM005307–HM005312) belonging to cluster 2 of group A, and 79.6% identity with the divergent isolate R5A from Réunion Island (L29569) belonging to phylogenetic group B of ZYMV. The presence of ZYMV in four distant locations in southern Ivory Coast suggests that this virus constitutes a serious threat to cucurbit production in this country. References: (1) C. Desbiez and H. Lecoq. Plant Pathol. 46:809, 1997, (2) C. Desbiez et al. Virus Res. 85:5, 2002, (3) H. Lecoq et al. Virus Res. 141:190, 2009.

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