Effects of thiazolidine-4(R)-carboxylates and other low-molecular-weight sulfur compounds on the activity of mercaptopyruvate sulfurtransferase, rhodanese and cystathionase in Ehrlich ascites tumor cells and tumor-bearing mouse liver

Amino Acids ◽  
1997 ◽  
Vol 12 (3-4) ◽  
pp. 309-314 ◽  
Author(s):  
M. Wróbel ◽  
L. Włodek
Keyword(s):  
Molecular Weight ◽  
Tumor Cells ◽  
Mouse Liver ◽  
Low Molecular Weight ◽  
Tumor Bearing Mouse ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Tumor Bearing ◽  
Ehrlich Ascites ◽  
Mercaptopyruvate Sulfurtransferase

Effect of Garlic, Chinese Medicinal Drugs and Amino Acids on Growth of Erlich Ascites Tumor Cells in Mice

1983 ◽  
Vol 11 (01n04) ◽  
pp. 69-73 ◽  
Author(s):  
Y.M. Choy ◽  
T.T. Kwok ◽  
K.P. Fung ◽  
C.Y. Lee
Keyword(s):  
Amino Acids ◽  
Tumor Cells ◽  
Peritoneal Cavity ◽  
Growth And Development ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Tumor Bearing ◽  
Ehrlich Ascites ◽  
Medicinal Drugs ◽  
Growth Of Tumor

A number of food materials or drugs have been screened for the effect on the growth and development of transplantable Ehrlich ascites tumor cells. Growth of tumor-bearing mice was significantly inhibited by feeding garlic as well as some amino acids. These materials significantly reduced the total number of free tumor cells growing in the peritoneal cavity of mice and prolonged significantly the length of time for 50% death of tumor-bearing mice.

The Partial Purification and Properties of Uridine Kinase from Ehrlich Ascites Tumor Cells

1973 ◽  
Vol 51 (4) ◽  
pp. 379-389 ◽  
Author(s):  
Gerald Krystal ◽  
Peter G. Scholefield
Keyword(s):  
Molecular Weight ◽  
Tumor Cells ◽  
Weight Fraction ◽  
Ehrlich Ascites Tumor ◽  
Partial Purification ◽  
Heat Inactivation ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Uridine Kinase ◽  
Ehrlich Ascites

In the partial purification of uridine kinase (ATP:uridine 5′-phosphotransferase, EC 2.7.1.48) from Ehrlich ascites tumor cells, two active fractions were obtained by chromatography on Sepharose 6B. Their molecular weights, as determined by a gel filtration and sucrose density centrifugation, were approximately 120 000 and 30 000. The larger molecular weight species was less stable on incubation at 60° and slightly less sensitive to CTP inhibition than the lower molecular weight fraction. Both fractions were stabilized against heat inactivation by ATP and CTP.The two fractions displayed similar apparent Km values for uridine and ATP, similar specificities for substrates and inhibitors, and similar divalent cation requirements.In the partial purification of uridine kinase from mouse intestine, only the larger molecular weight fraction was obtained by chromatography on Sepharose 6B.In addition, some preliminary evidence for a monomer–polymer relationship between the two molecular weight species is presented.

scholarly journals Effects of fixation and postfixation treatments on volume of injured cells;.

1975 ◽  
Vol 23 (4) ◽  
pp. 251-270 ◽  
Author(s):  
A Penttila ◽  
E M McDowell ◽  
B F Trump
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Tumor Cells ◽  
Phosphate Buffer ◽  
Sulfonic Acid ◽  
Osmium Tetroxide ◽  
Ehrlich Ascites Tumor ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Ehrlich Ascites

The effects of fixation with glutaraldehyde (GA), formaldehyde (FA), glutaraldehyde-formaldehyde (GA-FA), flutaraldehyde-osmium tetroxide (GA-Os04) and osmium tetroxide (OsO4) on cel volume were studied in control, p-chloromercuribenzene sulfonic acid (PCMBS)-treated and hypotonically-treated Ehrlich ascites tumor cells. Among the variables investigated were concentration of the tixative agent, osmolity of the buffer, total osmolaity of the fixation solution, osmolaltity of the postfixation buffer and the time of fixation and postfixation treatment; in addition, the effects of adding calcium and high molecular weight compounds to the fixative solution were studied. When the effects of standard fixatives on control, PCMBS- and hypotonically-treated cells were compared, marked differences were apparent in the behavior of control and injured cells. Control cells retained near prefixation volume in 3% GA and 3% GA-1% OsO4, swelled in 4% FA or 1% OsO4 and phosphate buffer (tkrp), whereas tpcmbs (310 mosM KRP)- and hypotonically-treated cells (103 mos M KRP) shrank in all aldehyde fixatives but swelled in 1% OsO4. Reducing the buffer osmolality had similar effects on control and injured cells although, there were variations in degree...

Immunopharmacological Studies of Low Molecular Weight Polysaccharide from Angelica Sinensis

1994 ◽  
Vol 22 (02) ◽  
pp. 137-145 ◽  
Author(s):  
Y. M. Choy ◽  
K. N. Leung ◽  
C. S. Cho ◽  
C. K. Wong ◽  
P. K. T. Pang
Keyword(s):  
Molecular Weight ◽  
Uronic Acid ◽  
Angelica Sinensis ◽  
Low Molecular Weight ◽  
Ascites Tumor ◽  
Tumor Bearing ◽  
Ehrlich Ascites ◽  
Anti Tumor Activity

A low molecular weight polysaccharide has been isolated from the rhizome of Angelica sinensis (Oliv.) Diels (Umbelliferaer). It has a molecular weight of approximately 3,000 and consists of protein (4.73%) and carbohydrate (85.85%) of which 5.2% is uronic acid. It shows strong anti-tumor activity on Ehrlich Ascites tumor bearing mice. It also exhibits immunostimulating activities, both in vitro and in vivo.

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