Protoplast fusion technology

1989 ◽  
Vol 12 (4) ◽  
pp. 157-161 ◽  
Author(s):  
Stephen Gleddie ◽  
Wilfred A. Keller
Keyword(s):  
Protoplast Fusion ◽  
Fusion Technology

Protoplast Fusion Technology- Somatic Hybridization and Cybridization

2010 ◽  
pp. 175-198 ◽  
Author(s):  
Jude W. Grosser ◽  
Milica alovi ◽  
Eliezer S. Louzada
Keyword(s):  
Protoplast Fusion ◽  
Somatic Hybridization ◽  
Fusion Technology

Selective Breeding of Oxygen-Tolerant and Oxalate-Degrading Lactic Acid Bacteria by Protoplast Fusion

2013 ◽  
Vol 750-752 ◽  
pp. 1489-1494 ◽  
Author(s):  
Sheng Chen ◽  
Yu Li ◽  
Wen Bin Jin ◽  
Yan Chen ◽  
Xiao Guang Liu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Protoplast Fusion ◽  
Selective Breeding ◽  
Fusion Rate ◽  
Fusion Technology ◽  
Optimum Conditions ◽  
Fusion Temperature ◽  
Bifidobacterium Lactis

Bifidobacterium lactiswith oxalate-degrading capacity can efficiently reduce the oxalate in vivo, and it can be used to prevent and treat kidney stone diseases. WhileBifidobacterium lactisis poorly oxygen-tolerant, which hinders it from being as microbial ecological agents. To obtain oxygen-tolerant and oxalate-degrading lactic acid bacteria, protoplast fusion technology was used betweenB. lactisandL. acidophilus.Under the optimum conditions of protoplast fusion with PEG 6000 concentration 50%, the fusion time 7 min, the fusion temperature 30°C, the concentration of CaCl20. 02mol/ L and the concentration of MgCl20.5mol/ L, the fusion rate reached 7.6%, and three oxygen-tolerant fusant showing that the level of oxalate degradation were similar withB. lactiswas obtained. The fusants of SZY1-7 and SZY2-1 could tolerance to pH 2.5 and 0.5% (w/v) bile salt.

scholarly journals Effect of Enzyme Treatments on Protoplast Isolation from Leaves of Vetiver (Vetiveria spp.)

2021 ◽  
Vol 20 (3) ◽  
Author(s):  
Sumana Leaungthitikanchana ◽  
Khachapohn Thongdonyod ◽  
Nootjaree Singphan
Keyword(s):  
Cell Wall ◽  
Protoplast Fusion ◽  
Incubation Time ◽  
Protoplast Culture ◽  
Protoplast Isolation ◽  
Fusion Technology ◽  
Vetiveria Zizanioides ◽  
Enzyme Solution ◽  
Protoplast Yield ◽  
High Viability

Protoplast isolation is a first and important step for establishing a new plant with desired traits through protoplast fusion technology. This experiments were conducted to evaluate various concentration of enzymes and incubation time on protoplast yield and viability in two vetiver ecotypes, Kamphaeng Phet 2 (Vetiveria zizanioides Nash) and Prachuap Khiri Khan (V. nemoralis A.Camus). The results revealed that protoplast yields were significantly affected by different enzyme treatments. The highest protoplast yield (6.12x105 protoplasts/ml) and high viability (98.61%) in Kamphaeng Phet 2 was obtained through the process of cell wall digestion when treated with enzyme solution containing 0.5% (w/v) cellulase onozuka R-10 and 0.5% (w/v) macerozyme R-10 in combination. While, the optimal enzyme solution for protoplast isolation from leaves of Prachuap Khiri Khan was the combination of 1.0% (w/v) cellulase onozuka R-10 and 0.4% (w/v) macerozyme R-10, resulting in the highest yield (6.80x105 protoplasts/ml) and viability (96.56%) of protoplasts. Meanwhile, incubation time of 24 h with the optimal enzyme solution resulted in the highest protoplast yields of both ecotypes. Our findings have the potential to generate an efficient protocol to isolate the protoplast from leaves of vetiver which can be used for further research studies in protoplast culture and fusion for vetiver improvement. Keywords: Cellulase onozuka R-10, Macerozyme R-10, Protoplast isolation, Vetiver

Genome shuffling for phenotypic improvement of industrial strains through recursive protoplast fusion technology

2021 ◽  
pp. 1-10
Author(s):  
Ravichandra Hospet ◽  
Devarajan Thangadurai ◽  
Natália Cruz-Martins ◽  
Jeyabalan Sangeetha ◽  
Konerira Aiyappa Anu Appaiah ◽  
...  
Keyword(s):  
Protoplast Fusion ◽  
Genome Shuffling ◽  
Fusion Technology ◽  
Industrial Strains
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