A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells

1977 ◽  
Vol 3 (2) ◽  
pp. 231-236 ◽  
Author(s):  
Malcolm L. Gefter ◽  
David H. Margulies ◽  
Matthew D. Scharff
Hybridoma ◽  
1989 ◽  
Vol 8 (2) ◽  
pp. 249-257 ◽  
Author(s):  
LOTTA HIRSCHBERG ◽  
GÖRAN BÖLSKE ◽  
TORD HOLME

1987 ◽  
Vol 7 (2) ◽  
pp. 936-939
Author(s):  
A Greenberg ◽  
R Ber ◽  
Z Kra-Oz ◽  
R Laskov

Adherent hybrids between immunoglobulin-producing mouse myeloma cells and fibroblasts do not produce immunoglobulin polypeptide chains. These hybrids retained the actively rearranged immunoglobulin genes of the myeloma parental cells but lacked immunoglobulin heavy- and light-chain RNA transcripts. We conclude that the shutoff of immunoglobulin production in these hybrids occurs at the transcription or early processing level.


Author(s):  
Ying Zhang ◽  
Xin‑Yu Lin ◽  
Jiao‑Hui Zhang ◽  
Zheng‑Lu Xie ◽  
Hui Deng ◽  
...  

2012 ◽  
Vol 67 (7) ◽  
pp. 717-724
Author(s):  
Shihua Song ◽  
Xiang Deng ◽  
Zhi Guan ◽  
Yanhong He

A simple method for the synthesis of phthalazinetrione derivatives by a one-pot three-component condensation reaction of phthalhydrazide, 1,3-dicarbonyl compounds and aldehydes catalyzed by the ionic liquid 4-(3-methyl-1-imidazolio)-1-butanesulfonic acid hydrogen sulfate ([BSO3HmIm]HSO4) is reported. Good to excellent yields were obtained in short reaction times in the solvent PEG 600 (polyethylene glycol 600) at 120 °C. The strategy proved to be efficient and environmentally benign. The catalyst/solvent system could easily be recovered and reused for at least 5 times without noticeable loss of activity


Biochemistry ◽  
1971 ◽  
Vol 10 (4) ◽  
pp. 659-667 ◽  
Author(s):  
Paul M. Knopf ◽  
Yong Sung Choi ◽  
Edwin S. Lennox

2006 ◽  
Vol 11 (4) ◽  
pp. 408-414 ◽  
Author(s):  
G ZHANG ◽  
H SHENG ◽  
J LIU

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