Bone marrow tissue and proliferation markers: Results and general problems

1993 ◽  
Vol 423 (6) ◽  
pp. 409-416 ◽  
Author(s):  
Juergen Thiele ◽  
Robert Fischer
Keyword(s):  
Bone Marrow ◽  
Proliferation Markers ◽  
Bone Marrow Tissue

scholarly journals Secondary haemophagocytic lymphohistiocytosis in COVID-19: correlation of the autopsy findings of bone marrow haemophagocytosis with HScore

2021 ◽  
pp. jclinpath-2020-207337
Author(s):  
Claudia Núñez-Torrón ◽  
Ana Ferrer-Gómez ◽  
Esther Moreno Moreno ◽  
Belen Pérez-Mies ◽  
Jesús Villarrubia ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Immune Response ◽  
Immune System ◽  
Multiorgan Failure ◽  
Histological Findings ◽  
Haemophagocytic Lymphohistiocytosis ◽  
Bone Marrow Tissue ◽  
Autopsy Findings ◽  
Specific Finding ◽  
Clinical Records

BackgroundSecondary haemophagocytic lymphohistiocytosis (sHLH) is characterised by a hyper activation of immune system that leads to multiorgan failure. It is suggested that excessive immune response in patients with COVID-19 could mimic this syndrome. Some COVID-19 autopsy studies have revealed the presence of haemophagocytosis images in bone marrow, raising the possibility, along with HScore parameters, of sHLH.AimOur objective is to ascertain the existence of sHLH in some patients with severe COVID-19.MethodsWe report the autopsy histological findings of 16 patients with COVID-19, focusing on the presence of haemophagocytosis in bone marrow, obtained from rib squeeze and integrating these findings with HScore parameters. CD68 immunohistochemical stains were used to highlight histiocytes and haemophagocytic cells. Clinical evolution and laboratory parameters of patients were collected from electronic clinical records.ResultsEleven patients (68.7%) displayed moderate histiocytic hyperplasia with haemophagocytosis (HHH) in bone marrow, three patients (18.7%) displayed severe HHH and the remainder were mild. All HScore parameters were collected in 10 patients (62.5%). Among the patients in which all parameters were evaluable, eight patients (80%) had an HScore >169. sHLH was not clinically suspected in any case.ConclusionsOur results support the recommendation of some authors to use the HScore in patients with severe COVID-19 in order to identify those who could benefit from immunosuppressive therapies. The presence of haemophagocytosis in bone marrow tissue, despite not being a specific finding, has proved to be a very useful tool in our study to identify these patients.

scholarly journals Hematopoiesis in 3 dimensions: human and murine bone marrow architecture visualized by confocal microscopy

Blood ◽  
2010 ◽  
Vol 116 (15) ◽  
pp. e41-e55 ◽  
Author(s):  
Tomoiku Takaku ◽  
Daniela Malide ◽  
Jichun Chen ◽  
Rodrigo T. Calado ◽  
Sachiko Kajigaya ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Three Dimensional ◽  
Cell Types ◽  
Bone Marrow Tissue ◽  
Murine Bone Marrow ◽  
3 Dimensional ◽  
Vascular Structures ◽  
Bone Structures ◽  
Surrounding Bone

AbstractIn many animals, blood cell production occurs in the bone marrow. Hematopoiesis is complex, requiring self-renewing and pluripotent stem cells, differentiated progenitor and precursor cells, and supportive stroma, adipose tissue, vascular structures, and extracellular matrix. Although imaging is a vital tool in hematology research, the 3-dimensional architecture of the bone marrow tissue in situ remains largely uncharacterized. The major hindrance to imaging the intact marrow is the surrounding bone structures are almost impossible to cut/image through. We have overcome these obstacles and describe a method whereby whole-mounts of bone marrow tissue were immunostained and imaged in 3 dimensions by confocal fluorescence and reflection microscopy. We have successfully mapped by multicolor immunofluorescence the localization pattern of as many as 4 cell features simultaneously over large tiled views and to depths of approximately 150 μm. Three-dimensional images can be assessed qualitatively and quantitatively to appreciate the distribution of cell types and their interrelationships, with minimal perturbations of the tissue. We demonstrate its application to normal mouse and human marrow, to murine models of marrow failure, and to patients with aplastic anemia, myeloid, and lymphoid cell malignancies. The technique should be generally adaptable for basic laboratory investigation and for clinical diagnosis of hematologic diseases.

scholarly journals Mechanisms of benzene-induced hematotoxicity and leukemogenicity: cDNA microarray analyses using mouse bone marrow tissue.

2003 ◽  
Vol 111 (11) ◽  
pp. 1411-1420 ◽  
Author(s):  
Byung-Il Yoon ◽  
Guang-Xun Li ◽  
Kunio Kitada ◽  
Yasushi Kawasaki ◽  
Katsuhide Igarashi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cdna Microarray ◽  
Mouse Bone Marrow ◽  
Bone Marrow Tissue ◽  
Microarray Analyses

scholarly journals Studies on Thrombopoiesis

Blood ◽  
1957 ◽  
Vol 12 (6) ◽  
pp. 507-519 ◽  
Author(s):  
G. IZAK ◽  
D. NELKEN ◽  
J. GUREVITCH ◽  
MISS A. HERZOG
Keyword(s):  
Bone Marrow ◽  
Tissue Culture ◽  
Guinea Pigs ◽  
Gradual Increase ◽  
Bone Marrow Tissue ◽  
Experimental Animals ◽  
Healthy Humans ◽  
The One

Abstract Thrombocyte production from megakaryocytes of healthy humans, dogs, guinea pigs and mice was observed continuously for one to six days in tissue culture. Approximately 70 per cent of the explanted megakaryocytes broke down to give rise to numerous platelets, while the remaining 30 per cent of the cells remained unchanged. The newly formed thrombocytes were separated from the rest of the bone marrow tissue, counted and their serotonin absorbing capacity determined. There was invariably a gradual increase in both the number of thrombocytes and in their serotonin absorbing capacity during the one to six days of observation. The results obtained were similar in human megakaryocytes and in those of experimental animals.

Prostaglandin E2 plays a key role in the immunosuppressive properties of adipose and bone marrow tissue-derived mesenchymal stromal cells

2010 ◽  
Vol 316 (19) ◽  
pp. 3109-3123 ◽  
Author(s):  
Rosa Yañez ◽  
Alberto Oviedo ◽  
Montserrat Aldea ◽  
Juan A. Bueren ◽  
María L. Lamana
Keyword(s):  
Bone Marrow ◽  
Prostaglandin E2 ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Bone Marrow Tissue

scholarly journals The construction of high efficiency human bone marrow tissue ex vivo

1991 ◽  
Vol 45 (3) ◽  
pp. 268-272 ◽  
Author(s):  
Stephen G. Emerson ◽  
Bernhard O. Palsson ◽  
Michael F. Clarke
Keyword(s):  
Bone Marrow ◽  
High Efficiency ◽  
Ex Vivo ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Tissue
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