Correlation of cytomegalovirus (CMV) detection in urine by tissue culture virus isolation, early antigen immunofluorescence test and nucleic acid hybridization

Infection ◽  
1988 ◽  
Vol 16 (3) ◽  
pp. 153-157 ◽  
Author(s):  
Gertraud Meyer ◽  
Gisela Enders
Keyword(s):  
Tissue Culture ◽  
Nucleic Acid ◽  
Virus Isolation ◽  
Nucleic Acid Hybridization ◽  
Immunofluorescence Test ◽  
Early Antigen ◽  
Culture Virus

Detection and identification of poliovirus in environmental samples using nucleic acid hybridization

1990 ◽  
Vol 36 (9) ◽  
pp. 664-669 ◽  
Author(s):  
David R. Preston ◽  
G. Rasul Chaudhry ◽  
Samuel R. Farrah
Keyword(s):  
Tissue Culture ◽  
Nucleic Acid ◽  
Environmental Samples ◽  
Nucleic Acid Hybridization ◽  
Dot Blot ◽  
Polio Virus ◽  
Detection And Identification ◽  
Specificity And Sensitivity ◽  
Hybridization Procedure ◽  
Great Specificity

A procedure was developed to effectively extract viral RNA from poliovirus tissue-culture lysates while eliminating the hybridization background associated with tissue cultures uninfected with poliovirus. Poliovirus cDNA cloned into a pUC vector was used as probe. Both the recombinant plasmids and the cDNA showed great specificity towards poliovirus. However, both probes hybridized with the single-stranded DNA coliphage [Formula: see text]. Tissue culture was found to be an effective method to increase the number of viruses found in environmental samples to a level detectable by hybridization procedures, whereas direct hybridization of RNA from unamplified and highly concentrated raw wastewater showed poor hybridization signals. The specificity and sensitivity of the hybridization procedure developed during these studies indicate that this method may be best suited for the identification rather than the detection of viruses isolated from environmental samples. Key words: nucleic acid hybridization, polio virus, water, dot blot.

Detection of cytomegalovirus by the early-antigen immunofluorescence test versus conventional tissue culture

1989 ◽  
Vol 8 (7) ◽  
pp. 610-613 ◽  
Author(s):  
I. Lautenschlager ◽  
J. Suni ◽  
J. Ahonen ◽  
C. Grönhagen-Riska ◽  
P. Ruutu ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Immunofluorescence Test ◽  
Early Antigen

Comparison of Poliovirus Detection in Water by Cell Culture and Nucleic Acid Hybridization

1993 ◽  
Vol 27 (3-4) ◽  
pp. 315-319
Author(s):  
Carlos E. Enriquez ◽  
Morteza Abbaszadegan ◽  
Ian L. Pepper ◽  
Kenneth J. Richardson ◽  
Aaron B. Margolin ◽  
...  
Keyword(s):  
Cell Culture ◽  
Tissue Culture ◽  
Nucleic Acid ◽  
Water Samples ◽  
Environmental Water ◽  
Nucleic Acid Hybridization ◽  
Well Water ◽  
Gene Probe ◽  
Hybridization Technique ◽  
Water Filter

The nucleic acid hybridization technique has been used to detect viral nucleic acid in environmental water samples. This type of assay, in contrast with tissue culture assays, may not distinguish between viable and non-viable viruses. We evaluated, by comparison with tissue culture infectivity assay (plaque forming method), the ability of the gene probe assay to detect viable poliovirus 1 (LSc) in well water, autoclaved well water, filter-sterilized well water and autoclaved phosphate buffered saline kept at 37° C and 15° C for 75 days, and in dechlorinated tapwater held at room temperature. A gradual decline in numbers of poliovirus was observed in all of the samples by cell culture assay. With the exception of autoclaved well water and phosphate buffer samples, a parallel decline in virus detectable by gene probe occurred in all other water samples.

Hierarchical Assembly of Viral Nanotemplates with Encoded Microparticles via Nucleic Acid Hybridization

Langmuir ◽  
2008 ◽  
Vol 24 (21) ◽  
pp. 12483-12488 ◽  
Author(s):  
Wui Siew Tan ◽  
Christina L. Lewis ◽  
Nicholas E. Horelik ◽  
Daniel C. Pregibon ◽  
Patrick S. Doyle ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Hybridization ◽  
Hierarchical Assembly

scholarly journals Characterization of a Severe Strain of Cucumber Mosaic Cucumovirus Seedborne in Cowpea

Plant Disease ◽  
1998 ◽  
Vol 82 (4) ◽  
pp. 419-422 ◽  
Author(s):  
A. G. Gillaspie ◽  
M. R. Hajimorad ◽  
S. A. Ghabrial
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Hybridization ◽  
Satellite Rna ◽  
Severe Strain ◽  
Cucumber Mosaic Cucumovirus ◽  
Hybridization Assays

A new seedborne strain of cucumber mosaic cucumovirus (CMV) that induces severe symptoms on many cowpea genotypes was detected in Georgia in 1994. This strain, designated CMV-Csb, is asymptomatic on tobacco, but it produces more severe cowpea stunt symptoms when present in combination with blackeye cowpea mosaic potyvirus than do the more prevalent CMV isolates. The new strain is seedborne in cowpea (1.5 to 37%), has no associated satellite RNA, and is classified as a member of subgroup I of CMV strains based on nucleic acid hybridization assays.

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