Prevention of postoperative wound infection after appendectomy by local application of tinidazole: A double-blind study

Abstract Background: Nitrous oxide inactivates methionine synthase and may lead to DNA damage and wound infection. By using single-cell gel electrophoresis (comet assay), the authors determined the effect of nitrous oxide on DNA damage in circulating leukocytes. Methods: In this double-blind, randomized controlled trial, 91 patients undergoing major colorectal surgery were randomized to receive 70% nitrous oxide (n = 31) or nitrous oxide-free anesthesia using 30 (n = 30) or 80% (n = 30) oxygen. Venous blood was collected before and 24 h after surgery. The primary outcome was extent of DNA damage, quantified as the percentage of DNA staining intensity in the comet tail using digital fluorescence microscopy. Incidence of postoperative wound infection was also recorded. Results: Nitrous oxide exposure was associated with a two-fold increase in the percentage of DNA intensity in tail (P = 0.0003), but not in the 30 (P = 0.181) or 80% oxygen groups (P = 0.419). There was a positive correlation between the duration of nitrous oxide exposure and extent of DNA damage, r = 0.33, P = 0.029. However, no correlation was observed in nitrous oxide-free patients. The proportions of postoperative wound infection, using the Centers for Disease Control and Prevention criteria, were 19.4% (6 of 31) in the 70% nitrous oxide group and 6.7% (2 of 30) in both the 30 and 80% oxygen groups, P = 0.21. An increase in DNA damage was associated with a higher risk of wound infection, adjusted odds ratio (95% CIs): 1.19 (1.07–1.34), P = 0.003. Conclusions: Nitrous oxide increased DNA damage compared with nitrous oxide-free anesthesia and was associated with postoperative wound infection.

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Local application of honey for treatment of neonatal postoperative wound infection

Acta Paediatrica ◽

10.1111/j.1651-2227.1998.tb01473.x ◽

2007 ◽

Vol 87 (4) ◽

pp. 429-432 ◽

Cited By ~ 34

Author(s):

A Vardi ◽

Z Barzilay ◽

N Linder ◽

HA Cohen ◽

G Paret ◽

...

Keyword(s):

Wound Infection ◽

Local Application ◽

Postoperative Wound Infection ◽

Postoperative Wound

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Prophylactic lincomycin in the prevention of wound infection following appendicectomy: A double blind study

British Journal of Surgery ◽

10.1002/bjs.1800631222 ◽

1976 ◽

Vol 63 (12) ◽

pp. 973-977 ◽

Cited By ~ 25

Author(s):

D. A. Leigh ◽

R. Pease ◽

H. Henderson ◽

K. Simmons ◽

R. Russ

Keyword(s):

Wound Infection ◽

Blind Study ◽

Double Blind Study ◽

Double Blind

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Local application of an antifibrinolytic in tonsillectomy A double-blind study

The Journal of Laryngology & Otology ◽

10.1017/s0022215100079081 ◽

1974 ◽

Vol 88 (6) ◽

pp. 565-568 ◽

Cited By ~ 9

Author(s):

J. Falbe-Hansen ◽

Bo Jacobsen ◽

E. Lorenzen

Keyword(s):

Blind Study ◽

Local Application ◽

Double Blind Study ◽

Double Blind

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A Double Blind Study on the Efficacy of Local Application of Hemocoagulase Solution in Wound Healing

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1334 ◽

2013 ◽

Vol 14 (3) ◽

pp. 394-400 ◽

Cited By ~ 2

Author(s):

Sachin Aslam ◽

PG Francis ◽

BHS Rao ◽

M Ummar ◽

JK Issac ◽

...

Keyword(s):

Wound Healing ◽

Epithelial Cells ◽

Maxillofacial Surgery ◽

Blind Study ◽

Local Application ◽

Dental Extraction ◽

Hemostatic Agent ◽

Oral And Maxillofacial Surgery ◽

Double Blind Study ◽

Double Blind

ABSTRACT Aim The present double blind study has been designed to evaluate the efficacy of local application of hemocoagulase solution as compared to a placebo in wound healing following dental extraction. Materials and methods A total of 20 patients who required dental extraction for orthodontic intervention were included. The hemocoagulase solution and a placebo were locally applied to the extraction sockets and the efficacy of the solution in terms of bleeding control, anti-inflammatory responses, its antiseptic properties and efficacy in wound healing were evaluated. Results The mean time required to achieve hemostasis was found to be 1.37 minutes in side A (test) and 2.33 minutes in side B (control) indicating that side A achieved faster hemostasis when compared to side B. At the 6th hour postoperatively, bleeding was not evident on either sides, and the amount of pain in side A was found to be less compared to side B. The number of RBCs, polymorphs, chronic inflammatory cells were not different in both the groups, whereas at 3rd postoperative day epithelial cells were greater in side A (test) compared to side B (control). Biopsy reports on the 12th postoperative day indicated that the number of fibroblasts, epithelial cells, collagen count was found to be greater in side A (test) compared to side B (control). Conclusion The topical hemocoagulase solution may be advocated in the field of oral and maxillofacial surgery, as a hemostatic agent and promoter of wound healing. However, further studies, with large number of cases and different clinical situations should be considered to authenticate the efficacy of this hemocoagulase solution in the practice of oral and maxillofacial surgery. Clinical significance Wound healing plays an important role in the success of any surgical procedure, such as extractions, and the hemocoagulase system may act as a hemostatic agent and a promoter of wound healing. How to cite this article Aslam S, Francis PG, Rao BHS, Ummar M, Issac JK, Nair RB. A Double Blind Study on the Efficacy of Local Application of Hemocoagulase Solution in Wound Healing. J Contemp Dent Pract 2013;14(3):394-400.

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