Characterization of calcium oxalate crystals in woody plants by X-ray microarea diffractometry

1987 ◽  
Vol 43 (9) ◽  
pp. 1006-1008 ◽  
Author(s):  
T. Taniguchi ◽  
H. Harada

2011 ◽  
Vol 24 (4) ◽  
pp. 733-740 ◽  
Author(s):  
Charles-Antoine Lamontagne ◽  
Gérard E. Plante ◽  
Michel Grandbois


2008 ◽  
Vol 45 (3) ◽  
pp. 417-426 ◽  
Author(s):  
M. E. Thompson ◽  
M. R. Lewin-Smith ◽  
V. F. Kalasinsky ◽  
K. M. Pizzolato ◽  
M. L. Fleetwood ◽  
...  


2007 ◽  
Vol 85 (5) ◽  
pp. 501-517 ◽  
Author(s):  
Walter P. Hartl ◽  
Helmut Klapper ◽  
Bruno Barbier ◽  
Hans Jürgen Ensikat ◽  
Richard Dronskowski ◽  
...  

The occurrence of various types of calcium oxalate crystals was studied in 251 species and subspecies of Cactaceae to determine whether they are useful characters for Cactaceae systematics. Crystal hydration states were identified by X-ray powder diffraction and polarizing microscopy as monoclinic calcium oxalate monohydrate (COM) and tetragonal calcium oxalate dihydrate (COD). Ninety-eight percent of taxa studied contained either COM or COD crystals, or both. Different morphologies of crystals were further defined by light microscopy and scanning electron microscopy as druses, raphides, styloids (prisms), and crystal sand. In particular, the preponderance of one of the hydration states (COM or COD) was characteristic for certain Cactus subfamilies. Data showed that in Pereskioideae, Maihuenioideae, and Opuntioideae COM is predominant, while in Cactoideae COD prevails. In the remainder of Cactoideae, the crystals were quite variable. In tribe Hylocereeae, many species form both COM and COD as well. In the genera Hylocereus , Epiphyllum , Selenicereus , and Weberocereus , COM forms were almost exclusively represented by raphides together with different crystal forms in their epidermal cells. In the remainder of the Cactoideae, crystals did not follow any observable patterns. Development of crystallographic standards for identifying crystal forms microscopically is proposed for future crystal studies.



Biologia ◽  
2007 ◽  
Vol 62 (1) ◽  
Author(s):  
Hatice Demiray

AbstractIn this study, Ca oxalate crystals were isolated from the leaves and X-ray diffraction identified them as weddelite in Crataegus pontica C. Koch, C. stevenii Polar., C. monogyna ssp. monogyna Jacq. in C. orientalis var. orientalis Pallas ex Bieb. both whewellite and weddelite crystals were found. Although there were some differences among the soluble and insoluble oxalate contents, they were not notable in the species of C. stevenii (10%; 18%), C. orientalis (12.4%; 15%), C. monogyna (12.9%; 13%), whereas in C. pontica the difference was so great with the lowest soluble (4%), and highest (28%) insoluble oxalate content. Crystals have tetragonal or prismatic shape in general but tetrahedral kinked and straight shapes were seen in C. orientalis, tetragonal aggregates in C. stevenii, and also pseudo-tetrahedral cordate (heart) shape were found in C. monogyna ssp. monogyna and C. pontica. As the crystal biomineralization is under genetic control, this characteristic hydration state of crystals of Crataegus orientalis var. orientalis must be important for systematic phylogenetics.



2009 ◽  
Vol 75 (22) ◽  
pp. 7079-7085 ◽  
Author(s):  
Melissa Marie S. Tuason ◽  
Joselito M. Arocena

ABSTRACT Piloderma fallax is an ectomycorrhizal fungus commonly associated with several conifer and hardwood species. We examined the formation of calcium oxalate crystals by P. fallax in response to calcium (0.0, 0.1, 0.5, 1, and 5 mM) and phosphorus (0.1 and 6 mM) additions in modified Melin-Norkrans agar medium. Both calcium and phosphorus supplementation significantly affected the amount of calcium oxalate formed. More calcium oxalate was formed at high P levels. Concentrations of soluble oxalate in the fungus and medium were higher at low P levels. There was a strong positive linear relationship between Ca level and calcium oxalate but only under conditions of phosphorus limitation. Calcium oxalate crystals were identified as the monohydrate form (calcium oxalate monohydrate [COM] whewellite) by X-ray diffraction analysis. Prismatic, styloid, and raphide forms of the crystals, characteristic COM, were observed on the surface of fungal hyphae by scanning electron microscopy. P. fallax may be capable of dissolving hyphal calcium oxalate under conditions of limited Ca. The biomineralization of calcium oxalate by fungi may be an important step in the translocation and cycling of Ca and P in soil.



2003 ◽  
Vol 160 (3) ◽  
pp. 239-245 ◽  
Author(s):  
David Jáuregui-Zúñiga ◽  
JuanPablo Reyes-Grajeda ◽  
JoséDavid Sepúlveda-Sánchez ◽  
JohnR. Whitaker ◽  
Abel Moreno


Author(s):  
Yuuko Ishii ◽  
Kazuyoshi Takiyama

Calcium oxalate crystals, which were produced in plants to protect protoplasm from the toxic action of oxalic acid formed by metabolism, were observed with an optical microscope. In the present investigation, calcium oxalate crystals isolated from many plants were studied from the viewpoint of crystal chemistry.Leaves of spinach, begonia, begonia lucerna, garden sorrel, goose foot, camellia, tea-plant, agave, et al., stem of aloe, and barks of cherry-tree, plum-tree, fir, camphor-tree et al. were used in the investigation. Plants were cut into small pieces and pulverized in water with a food blender. The mixture was filtered through gauze and water was sprinkled onto the residue. Many small crystalline particles sank in the filtrate. Particles were carefully gathered into a beaker. Dried particles were observed with a scanning electron microscope(SEM) and analyzed with an X-ray diffraction(XRD).



2016 ◽  
Vol 12 (3) ◽  
pp. 879-889 ◽  
Author(s):  
Kitisak Sintiprungrat ◽  
Nilubon Singhto ◽  
Visith Thongboonkerd

This is the first study to characterize changes in the secretome of human monocytes induced by calcium oxalate crystals.



2003 ◽  
Vol 51 (5) ◽  
pp. 565 ◽  
Author(s):  
Andrew J. Macnish ◽  
Donald E. Irving ◽  
Daryl C. Joyce ◽  
Vasanthe Vithanage ◽  
Alan H. Wearing ◽  
...  

Intracellular inclusions in the pedicel and calyx-tube tissues of Chamelaucium uncinatum Schauer (Myrtaceae) flowers are irregular in shape. They were shown, by polarised light and scanning electron microscopy, to be birefringent 8.9–29.5 μm druse (i.e. aggregate) crystals. Energy-dispersive X-ray spectroscopy showed that these crystals were predominantly composed of calcium. Histochemical and acid-solubility tests indicated that the crystals were calcium oxalate. Raman microprobe spectroscopy was used to confirm this chemical identity. The calcium oxalate crystals were located in xylem-vessel lumens and also in parenchyma cells adjacent to vascular tissues. Thus, the crystals may function to regulate soluble calcium concentrations in C. uncinatum tissues near sites where calcium is unloaded from the xylem.



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