Pancreatic islet cell suspensions from newborn rats; different preparation procedures, viability and (pro)insulin biosynthesis

1983 ◽  
Vol 39 (2) ◽  
pp. 210-212 ◽  
Author(s):  
D. Schröder ◽  
B. Hehmke ◽  
H. Zühlke
Keyword(s):  
Pancreatic Islet ◽  
Islet Cell ◽  
Cell Suspensions ◽  
Insulin Biosynthesis ◽  
Newborn Rats ◽  
Pancreatic Islet Cell

Characterization of pseudo-islets formed from pancreatic islet cell suspensions of neonatal rats

1983 ◽  
Vol 32 (2-3) ◽  
pp. 179-193 ◽  
Author(s):  
D. Schröder ◽  
U. Wegner ◽  
W. Besch ◽  
H. Zühlke
Keyword(s):  
Pancreatic Islet ◽  
Islet Cell ◽  
Cell Suspensions ◽  
Neonatal Rats ◽  
Pancreatic Islet Cell

333-LB: Impact of Aging on Mitochondrial Respiratory Chain Expression and Pancreatic Islet Cell Composition by Using a Mitochondrial DNA Mutator Mouse Model

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 333-LB
Author(s):  
XUEFEI YU ◽  
CATHERINE ARDEN ◽  
CHUN CHEN ◽  
CARLA BRADSHAW ◽  
JULIA WHITEHALL ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Mouse Model ◽  
Respiratory Chain ◽  
Pancreatic Islet ◽  
Islet Cell ◽  
Mitochondrial Respiratory Chain ◽  
Pancreatic Islet Cell ◽  
Cell Composition ◽  
Mitochondrial Respiratory

Islet-on-a-chip: Biomimetic micropillar-based microfluidic system for three-dimensional pancreatic islet cell culture

2021 ◽  
Vol 183 ◽  
pp. 113215
Author(s):  
Patrycja Sokolowska ◽  
Kamil Zukowski ◽  
Justyna Janikiewicz ◽  
Elzbieta Jastrzebska ◽  
Agnieszka Dobrzyn ◽  
...  
Keyword(s):  
Cell Culture ◽  
Pancreatic Islet ◽  
Islet Cell ◽  
Three Dimensional ◽  
Microfluidic System ◽  
Pancreatic Islet Cell ◽  
Islet Cell Culture

Diarrhea associated with pancreatic islet-cell tumors

1964 ◽  
Vol 9 (2) ◽  
pp. 97-108 ◽  
Author(s):  
Julien Deleu ◽  
Hendrik Tytgat ◽  
Guy E. Goidsenhoven
Keyword(s):  
Pancreatic Islet ◽  
Islet Cell ◽  
Islet Cell Tumors ◽  
Pancreatic Islet Cell ◽  
Pancreatic Islet Cell Tumors

scholarly journals Non-Islet Origin of Pancreatic Islet Cell Tumors

2004 ◽  
Vol 89 (4) ◽  
pp. 1934-1938 ◽  
Author(s):  
Alexander O. Vortmeyer ◽  
Steve Huang ◽  
Irina Lubensky ◽  
Zhengping Zhuang
Keyword(s):  
Pancreatic Islet ◽  
Islet Cell ◽  
Islet Cell Tumors ◽  
Pancreatic Islet Cell ◽  
Pancreatic Islet Cell Tumors

Single-cell chromatin accessibility identifies pancreatic islet cell type– and state-specific regulatory programs of diabetes risk

2021 ◽  
Author(s):  
Joshua Chiou ◽  
Chun Zeng ◽  
Zhang Cheng ◽  
Jee Yun Han ◽  
Michael Schlichting ◽  
...  
Keyword(s):  
Single Cell ◽  
Pancreatic Islet ◽  
Islet Cell ◽  
Chromatin Accessibility ◽  
Diabetes Risk ◽  
Cell Type ◽  
Pancreatic Islet Cell

scholarly journals Immunohistochemistry of Pancreatic Islet Cell Tumors in the Ferret (Mustela putorius furo)

1997 ◽  
Vol 34 (5) ◽  
pp. 387-393 ◽  
Author(s):  
G. A. Andrews ◽  
N. C. Myers ◽  
C. Chard-Bergstrom
Keyword(s):  
Pancreatic Islets ◽  
Pancreatic Islet ◽  
Pancreatic Polypeptide ◽  
Islet Cell ◽  
Islet Cells ◽  
Neuron Specific Enolase ◽  
Islet Cell Tumors ◽  
Pancreatic Islet Cell ◽  
Formalin Fixed Paraffin Embedded ◽  
Pancreatic Islet Cell Tumors

Twenty-two pancreatic islet cell tumors and normal pancreatic islets from ferrets were evaluated by immunohistochemistry for expression of the peptide hormones insulin, somatostatin, glucagon, and pancreatic polypeptide (PP) and the neuroendocrine markers chromogranin A (CgA) and neuron-specific enolase (NSE). In normal pancreatic islets, the majority of cells stained strongly with CgA and NSE. A cells, B cells, D cells, and PP cells stained strongly with glucagon, insulin, somatostatin, and PP, respectively. All 22 tumors stained with CgA and NSE. The proportion of cells within tumors staining for CgA was variable, but more than half of the cells stained positively in 18 of the tumors. The intensity of staining for CgA was strong (reactivity equivalent to or greater than normal islet cells in adjacent tissue) in 11 moderate in six, and weak in five of the tumors. All tumors stained for NSE, with ≥50% of the cells staining in 21 of the tumors, and the intensity of staining was strong in 18 of the tumors. Twenty of 22 tumors stained positively for insulin, with ≥50% of the cells staining in 19 of them. The intensity of staining for insulin was strong in 12, moderate in seven, and weak in one of the tumors. Approximately ≤1% of the cells in 15 of 22 tumors stained for somatostatin, five tumors stained for pancreatic polypeptide, and three tumors stained for glucagon. These data indicate that the majority of islet cell tumors of ferrets express immunohistochemically detectable insulin. CgA and NSE are both useful general markers for such tumors, including those that are insulin negative. Commercially available antisera to CgA, NSE, insulin, glucagon, somatostatin, and PP work well in formalin-fixed, paraffin-embedded tissue for immunophenotyping islet cell tumors in the ferret.

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