Broad bean wilt virus: Host range, purification, serology, transmission characteristics, and occurrence in faba bean in West Asia and North Africa

1990 ◽  
Vol 96 (5) ◽  
pp. 291-300 ◽  
Author(s):  
K. M. Makkouk ◽  
S. G. Kumari ◽  
L. Bos
Keyword(s):  
Host Range ◽  
North Africa ◽  
Faba Bean ◽  
Broad Bean ◽  
Transmission Characteristics ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus ◽  
Virus Host Range

The serological relationships and some other properties of isolates of broad bean wilt virus from faba bean and pea in China

1988 ◽  
Vol 113 (2) ◽  
pp. 287-296 ◽  
Author(s):  
Z. G. XU ◽  
A. J. COCKBAIN ◽  
R. D. WOODS ◽  
D. A. GOVIER
Keyword(s):  
Faba Bean ◽  
Broad Bean ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus

scholarly journals Broad bean mottle virus: Identification, host range, serology, and occurrence on faba bean (Vicia faba) in West Asia and North Africa

1989 ◽  
Vol 94 (4) ◽  
pp. 195-212 ◽  
Author(s):  
K. M. Makkouk ◽  
L. Bos ◽  
A. Rizkallah ◽  
O. I. Azzam ◽  
L. Katul
Keyword(s):  
Host Range ◽  
Vicia Faba ◽  
North Africa ◽  
Faba Bean ◽  
Broad Bean ◽  
Mottle Virus ◽  
Virus Identification ◽  
Broad Bean Mottle Virus

scholarly journals First Report of Broad bean wilt virus 2 in Leonurus sibiricus in Korea

Plant Disease ◽  
2014 ◽  
Vol 98 (12) ◽  
pp. 1748-1748 ◽  
Author(s):  
J.-K. Seo ◽  
O. J. Shin ◽  
H.-R. Kwak ◽  
M.-K. Kim ◽  
H.-S. Choi ◽  
...  
Keyword(s):  
Host Range ◽  
Broad Bean ◽  
Rt Pcr ◽  
Total Rna ◽  
First Report ◽  
Leonurus Sibiricus ◽  
Plant Pathol ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus ◽  
Test Plants

Leonurus sibiricus L. (family Lamiaceae) has been used as a traditional herbal remedy to treat various gynecologic diseases. Although it is a widely distributed subtropical weed in Southeast Asia, L. sibiricus have been commercially cultivated on a small scale in many geographic areas of Korea. In August 2012, field-grown L. sibiricus plants showing mosaic, yellowing, and stunting symptoms were collected near a pepper field in Andong, Korea. Since L. sibiricus is only consumed as a raw material of traditional medicine in Korea, symptomatic plants lose commercial value entirely. To identify the causal agent(s) of the virus-like symptoms, total RNA was extracted from the symptomatic leaves, and a transcriptome library was generated using the TruSeq Stranded Total RNA with Ribo-Zero plant kit (Illumina, San Diego, CA) according to the standard protocol. Next-generation sequencing (NGS) was performed using an Illumina HiSeq2000 sequencer. De novo assembly of the quality filtered NGS reads (101-bp paired-end reads) were performed using the Trinity pipeline and the assembled contigs (92,329 contigs) were analyzed against the viral reference genome database in GenBank by BLASTn and BLASTx searches (3). The entire NGS procedure was performed by Macrogen Inc. (Seoul, South Korea). Among the analyzed contigs, only two large contigs were clearly of viral origin. Nucleotide blast searches showed that the first and second contigs (5,914 and 3,534 bp, respectively) have maximum identities of 91 and 95% to RNA1 of the isolate RP3 (GenBank Accession No. JX183225) and RNA2 of the isolate RP7 (JX183234) of Broad bean wilt virus 2 (BBWV-2), which were isolated from pepper in Korea. The NGS results were confirmed by analyzing the sequences of the fragments covering the entire BBWV-2 genome amplified by RT-PCR using specific primers for BBWV-2 as described previously (1). To obtain the complete genome sequence, terminal sequences of both RNA segments were analyzed by the 5′ and 3′ rapid amplification of cDNA ends (RACE) method as described previously (1). The assembled full-length sequences of BBWV-2 RNA1 and RNA2 isolated from L. sibiricus were 5,951 and 3,575 nucleotides in length, respectively, and deposited in GenBank under the accessions KM076648 and KM076649, respectively. BBWV-2 belongs to the genus Fabavirus in the family Secoviridae and it is known to have a wide host range. To investigate the host range of the BBWV-2 isolated from L. sibiricus, sap from the symptomatic leaves of L. sibiricus was inoculated to the test plants including Nicotiana benthamiana, Capsicum annuum (red pepper), and C. annuum var. gulosum (Paprika). RT-PCR detection and sequencing of the amplicons showed that all the inoculated test plants were infected with the BBWV-2 isolated from L. sibiricus. Currently, BBWV-2 is epidemic in pepper fields in Korea (1,2). Because BBWV-2 is easily transmitted by various aphids, and L. sibiricus is widely distributed in both wild and cultivated fields in Korea, this host might serve as a potential source of BBWV-2 to other crops such as pepper. To the best of our knowledge, this is the first report of BBWV-2 in L. sibiricus. References: (1) H.-R. Kwak et al. Plant Pathol. J. 29:274, 2013. (2) H.-R. Kwak et al. Plant Pathol. J. 29:397, 2013. (3) S.-E. Schelhorn et al. PLoS Comput. Biol. 9:e1003228, 2013.

Intracellular Inclusion Bodies of Broad Bean Wilt Virus

1974 ◽  
Vol 80 (2) ◽  
pp. 127-135 ◽  
Author(s):  
D. Miličić ◽  
Mercedes Wrischer ◽  
N. Juretić
Keyword(s):  
Inclusion Bodies ◽  
Broad Bean ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus ◽  
Intracellular Inclusion

Broad bean wilt virus 1. [Distribution map].

2017 ◽  
Author(s):  
Keyword(s):  
New York ◽  
Czech Republic ◽  
New Zealand ◽  
North America ◽  
Broad Bean ◽  
Distribution Map ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus ◽  
New Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Broad bean wilt virus 1. Picornavirales: Secoriviridae: Fabavirus. Hosts: various. Information is given on the geographical distribution in Europe (Belgium, Bulgaria, Czech Republic, Germany, Italy, Netherlands, Slovenia, Spain and UK), Asia (Japan, Lebanon, Singapore and Syria), North America (USA, New York) and Oceania (New Zealand).

scholarly journals First report of Broad bean wilt virus 1 in Slovenia

2008 ◽  
Vol 57 (2) ◽  
pp. 395-395 ◽  
Author(s):  
N. Mehle ◽  
M. Tušek Žnidarič ◽  
T. Tornos ◽  
M. Ravnikar
Keyword(s):  
Broad Bean ◽  
First Report ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus

Effect of cucumber mosaic virus nd broad bean wilt virus n pepper yield

1999 ◽  
Vol 32 (6) ◽  
pp. 453-469
Author(s):  
Dimitrinka Hristova ◽  
Svetla Maneva
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Broad Bean ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus

Foliar Ringspot Disease ofThunbergia alataCaused by Broad Bean Wilt Virus

Plant Disease ◽  
1985 ◽  
Vol 69 (8) ◽  
pp. 726f ◽  
Author(s):  
R. Provvidenti
Keyword(s):  
Broad Bean ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus
Sign in / Sign up

Export Citation Format

Share Document