Sodium chloride, osmolyte, and osmolarity effects on blastocyst formation in bovine embryos produced by in vitro fertilization (IVF) and cultured in simple serum-free media

1996 ◽  
Vol 13 (7) ◽  
pp. 562-568 ◽  
Author(s):  
Zishu Liu ◽  
Robert H. Foote
Keyword(s):  
Sodium Chloride ◽  
In Vitro Fertilization ◽  
Bovine Embryos ◽  
Blastocyst Formation ◽  
Vitro Fertilization ◽  
Serum Free ◽  
Free Media

scholarly journals Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes

Reproduction ◽  
1988 ◽  
Vol 83 (2) ◽  
pp. 753-758 ◽  
Author(s):  
K. Goto ◽  
Y. Kajihara ◽  
S. Kosaka ◽  
M. Koba ◽  
Y. Nakanishi ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Cumulus Cells ◽  
Bovine Embryos ◽  
Vitro Fertilization

scholarly journals Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e108139 ◽  
Author(s):  
Maria Jesús Cánepa ◽  
Nicolás Matías Ortega ◽  
Melisa Carolina Monteleone ◽  
Nicolas Mucci ◽  
German Gustavo Kaiser ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Somatic Cell ◽  
Expression Profile ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Developmental Competence ◽  
Bovine Embryos ◽  
Vitro Fertilization

scholarly journals Comparison of Two Different Serum-free Media for In Vitro Culture of Bovine Embryos

2014 ◽  
Vol 29 (3) ◽  
pp. 229-234
Author(s):  
Se-woong Kim ◽  
Yeon-Gil Jung ◽  
Jong-im Park ◽  
Sangho Roh
Keyword(s):  
In Vitro Culture ◽  
Bovine Embryos ◽  
Serum Free ◽  
Free Media

Male-dependent variability of fertilization and embryo development in two bovine in vitro fertilization systems and the effects of casein phosphopeptides (CPPs)

1997 ◽  
Vol 9 (4) ◽  
pp. 465 ◽  
Author(s):  
U. Kreysing ◽  
T. Nagai ◽  
H. Niemann
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Individual Variability ◽  
Bovine Embryo ◽  
Blastocyst Formation ◽  
Embryo Production ◽  
Vitro Fertilization ◽  
Casein Phosphopeptides ◽  
Fertilization System

This study investigated the effects of semen from five different bulls and two different ejaculates of the same bull on penetration, cleavage, blastocyst formation, and cell allocation in bovine blastocysts produced in vitro. Casein phosphopeptides (CPPs) were tested for their ability to enhance fertilization and minimize variability among bulls and ejaculates. In Experiment 1, the BO-fertilization system was employed. Penetration and polyspermy both displayed great variation among bulls and between ejaculates, whereas no significant differences were observed in cleavage and blastocyst-formation rates. Similar variability was observed in penetration, polyspermy, cleavage, blastocyst-formation rates and cell allocation and distribution when the two fertilization systems, TALP and BO, were compared in Experiment 2. The BO-system supported penetration and polyspermy better (P < 0·05) than the TALP-system, whereas the TALP-system was superior (P < 0·05) in supporting cleavage and blastocyst formation. Significant interactions existed between bulls and the fertilization system employed. It is concluded that the success of in vitrofertilization is markedly dependent on individual bulls as well as on ejaculates from the same bull. CPPs are able to enhance penetration and embryo development in certain bulls or ejaculates and thus contribute to reducing the degree of individual variability, but they do not generally improve the success of bovine embryo production in vitro.

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