Cell wall assembly ofNeurospora crassa: Isolation and analysis of cell wall-less mutants

1990 ◽  
Vol 21 (4) ◽  
pp. 233-242 ◽  
Author(s):  
Patricia Phelps ◽  
Tracey Stark ◽  
Claude P. Selitrennikoff
Keyword(s):  
Cell Wall ◽  
Cell Wall Assembly ◽  
Ofneurospora Crassa ◽  
Wall Assembly

The role of the exocytic pathway in cell wall assembly in yeast

Yeast ◽  
2021 ◽  
Author(s):  
Qingguo Guo ◽  
Na Meng ◽  
Guanzhi Fan ◽  
Dong Sun ◽  
Yuan Meng ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Wall Assembly ◽  
Wall Assembly ◽  
Exocytic Pathway

scholarly journals Properties of Arabinogalactan Proteins (AGPs) in Apple (Malus × Domestica) Fruit at Different Stages of Ripening

Biology ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 225
Author(s):  
Agata Leszczuk ◽  
Justyna Cybulska ◽  
Tomasz Skrzypek ◽  
Artur Zdunek
Keyword(s):  
Cell Wall ◽  
Malus Domestica ◽  
Arabinogalactan Proteins ◽  
Ex Situ ◽  
Morphological Properties ◽  
Mature Stage ◽  
Fruit Storage ◽  
Cell Wall Assembly ◽  
Wall Assembly

Arabinogalactan proteins (AGPs) are constituents of the cell wall–plasma membrane continuum in fruit tissue. The aim of the study was to characterise AGPs contained in fruit by determination of their chemical structure and morphological properties. The results were obtained from in and ex situ investigations and a comparative analysis of AGPs present in Malus × domestica fruit at different stages of ripening from green fruit through the mature stage to over-ripening during fruit storage. The HPLC and colorimetric methods were used for analyses of the composition of monosaccharides and proteins in AGPs extracted from fruit. We have found that AGPs from fruit mainly consists of carbohydrate chains composed predominantly of arabinose, galactose, glucose, galacturonic acid, and xylose. The protein moiety accounts for 3.15–4.58%, which depends on the various phases of ripening. Taken together, our results show that the structural and morphological properties of AGPs and calcium concentration in AGPs are related to the progress of ripening, which is correlated with proper fruit cell wall assembly. In line with the existing knowledge, our data confirmed the typical carbohydrate composition of AGPs and may be the basis for studies regarding their presumed properties of binding calcium ions.

scholarly journals Chlamydomonas reinhardtii Has Multiple Prolyl 4-Hydroxylases, One of Which Is Essential for Proper Cell Wall Assembly

2007 ◽  
Vol 19 (1) ◽  
pp. 256-269 ◽  
Author(s):  
Katriina Keskiaho ◽  
Reija Hieta ◽  
Raija Sormunen ◽  
Johanna Myllyharju
Keyword(s):  
Cell Wall ◽  
Chlamydomonas Reinhardtii ◽  
Cell Wall Assembly ◽  
Wall Assembly

Do polyamines contribute to plant cell wall assembly by forming amide bonds with pectins?

2005 ◽  
Vol 66 (21) ◽  
pp. 2581-2594 ◽  
Author(s):  
Marcello Lenucci ◽  
Gabriella Piro ◽  
Janice G. Miller ◽  
Giuseppe Dalessandro ◽  
Stephen C. Fry
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Amide Bonds ◽  
Cell Wall Assembly ◽  
Wall Assembly

scholarly journals Assemblies of DivIVA Mark Sites for Hyphal Branching and Can Establish New Zones of Cell Wall Growth in Streptomyces coelicolor

2008 ◽  
Vol 190 (22) ◽  
pp. 7579-7583 ◽  
Author(s):  
Antje Marie Hempel ◽  
Sheng-bing Wang ◽  
Michal Letek ◽  
José A. Gil ◽  
Klas Flärdh
Keyword(s):  
Cell Wall ◽  
Streptomyces Coelicolor ◽  
Time Lapse ◽  
Cell Wall Assembly ◽  
Peptidoglycan Synthesis ◽  
Wall Growth ◽  
Lateral Branches ◽  
Wall Assembly ◽  
Time Lapse Imaging

ABSTRACT Time-lapse imaging of Streptomyces hyphae revealed foci of the essential protein DivIVA at sites where lateral branches will emerge. Overexpression experiments showed that DivIVA foci can trigger establishment of new zones of cell wall assembly, suggesting a key role of DivIVA in directing peptidoglycan synthesis and cell shape in Streptomyces.

scholarly journals Dynamics of cell wall assembly during early embryogenesis in the brown algaFucus

2016 ◽  
Vol 67 (21) ◽  
pp. 6089-6100 ◽  
Author(s):  
Thomas A. Torode ◽  
Amandine Siméon ◽  
Susan E. Marcus ◽  
Murielle Jam ◽  
Marie-Anne Le Moigne ◽  
...  
Keyword(s):  
Cell Wall ◽  
Early Embryogenesis ◽  
Cell Wall Assembly ◽  
Wall Assembly

scholarly journals The RIM101 Pathway Contributes to Yeast Cell Wall Assembly and Its Function Becomes Essential in the Absence of Mitogen-Activated Protein Kinase Slt2p

2006 ◽  
Vol 5 (3) ◽  
pp. 507-517 ◽  
Author(s):  
F. Castrejon ◽  
A. Gomez ◽  
M. Sanz ◽  
A. Duran ◽  
C. Roncero
Keyword(s):  
Cell Wall ◽  
Protein Kinase ◽  
Yeast Cell ◽  
Cell Walls ◽  
Active Form ◽  
Sodium Dodecyl ◽  
Phenotypic Traits ◽  
Compensatory Response ◽  
Cell Wall Assembly ◽  
Wall Assembly

ABSTRACT The Saccharomyces cerevisiae ynl294cΔ (rim21Δ) mutant was identified in our lab owing to its moderate resistance to calcofluor, although it also displayed all of the phenotypic traits associated with its function as the putative sensor (Rim21p) of the RIM101 pathway. rim21Δ also showed moderate hypersensitivity to sodium dodecyl sulfate, caffeine, and zymolyase, and the cell wall compensatory response in this mutant was very poor, as indicated by the almost complete absence of Slt2 phosphorylation and the modest increase in chitin synthesis after calcofluor treatment. However, the cell integrity pathway appeared functional after caffeine treatment or thermal stress. rim21Δ and rim101Δ mutant strains shared all of the cell-wall-associated phenotypes, which were reverted by the expression of Rim101-531p, the constitutively active form of this transcription factor. Therefore, the absence of a functional RIM101 pathway leads to cell wall defects. rim21Δ, as well as rim101Δ, was synthetic lethal with slt2Δ, a synthetic defect alleviated by osmotic stabilization of the media. The double mutants grown in osmotically stabilized media were extremely hypersensitive to zymolyase and showed thicker cell walls, with poorly defined mannoprotein layers. In contrast, rim21Δ rlm1Δ and rim101Δ rlm1Δ double mutants were fully viable. Taken together, these results show that the RIM101 pathway participates directly in cell wall assembly and that it acts in parallel with the protein kinase C pathway (PKC) in this process independently of the transcriptional effect of the compensatory response mediated by this route. In addition, these results provide new experimental evidence of the direct involvement of the PKC signal transduction pathway through the Sltp2 kinase in the construction of yeast cell walls.

Sign in / Sign up

Export Citation Format

Share Document