In vitro induction of vegetative buds by tobacco smoke condensate

Calli obtained from haploid and diploid plants of Nicotiana tabacum were inoculated on nutrient media supplemented with various concentrations of chelating agents: iron salt of ethylenediamine-di-o-hydroxyphenylacetic acid (Fe-EDDHA), 1,3-diamino-2-hydroxypropane-N,N,N′,N′-tetraacetic acid (DHPTA), 1,2-cyclohexanediaminetetraacetic acid (CYDA), citric acid, and tartaric acid. No auxin or cytokinin was added to the medium. Vegetative buds differentiated on the callus obtained from the haploid plants. However, these chelating agents proved ineffective in inducing differentiation on the callus derived from diploid plants. Morphogenetically, Fe-EDDHA and DHPTA proved more potent than CYDA, citric acid, and tartaric acid.

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In-vitro induction of vegetative buds by benz(a)anthracene in tobacco callus

Planta ◽

10.1007/bf00386137 ◽

1970 ◽

Vol 94 (3) ◽

pp. 246-249 ◽

Cited By ~ 11

Author(s):

T. S. Kochhar ◽

P. R. Bhalla ◽

P. S. Sabharwal

Keyword(s):

Tobacco Callus ◽

Vegetative Buds ◽

In Vitro Induction

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Potent in vitro inhibitors of tyrosinase: Metal-binding fractions of tobacco smoke condensate

Toxicology and Applied Pharmacology ◽

10.1016/0041-008x(74)90212-9 ◽

1974 ◽

Vol 27 (2) ◽

pp. 415-421 ◽

Cited By ~ 2

Author(s):

Vincent N. Finelli ◽

Peter M. Eller ◽

Patricia J. Montague ◽

Harold G. Petering

Keyword(s):

Tobacco Smoke ◽

Metal Binding ◽

Tobacco Smoke Condensate

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In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100050676 ◽

1974 ◽

Vol 32 ◽

pp. 132-133

Author(s):

John J. Wolosewick ◽

John H. D. Bryan

Keyword(s):

Endoplasmic Reticulum ◽

Smooth Endoplasmic Reticulum ◽

Nuclear Ring ◽

Rough Er ◽

Distal Direction ◽

In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

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Adverse Influence of Cigarette Smoking on the Endothelium

Thrombosis and Haemostasis ◽

10.1055/s-0038-1649654 ◽

1993 ◽

Vol 70 (04) ◽

pp. 707-711 ◽

Cited By ~ 77

Author(s):

Andrew D Blann ◽

Charles N McCollum

Keyword(s):

Endothelial Cells ◽

Von Willebrand Factor ◽

Tobacco Smoke ◽

Lipid Peroxides ◽

Short Exposure ◽

Acute Effects ◽

Adverse Influence ◽

Von Willebrand ◽

Willebrand Factor

SummaryThe effect of smoking on the blood vessel intima was examined by comparing indices of endothelial activity in serum from smokers with that from non-smokers. Serum from smokers contained higher levels of von Willebrand factor (p <0.01), the smoking markers cotinine (p <0.02) and thiocyanate (p <0.01), and was more cytotoxic to endothelial cells in vitro (p <0.02) than serum from non-smokers. The acute effects of smoking two unfiltered medium tar cigarettes was to briefly increase von Willebrand factor (p <0.001) and cytotoxicity of serum to endothelial cells in vitro (p <0.005), but lipid peroxides or thiocyanate were not increased by this short exposure to tobacco smoke. Although there were correlations between von Willebrand factor and smokers consumption of cigarettes (r = 0.28, p <0.02), number of years smoking (r = 0.41, p <0.001) and cotinine (r = 0.45, p <0.01), the tissue culture of endothelial cells with physiological levels of thiocyanate or nicotine suggested that these two smoking markers were not cytotoxic. They are therefore unlikely to be directly responsible for increased von Willebrand factor in the serum of smokers. We suggest that smoking exerts a deleterious influence on the endothelium and that the mechanism is complex.

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