Effect of temperature on symptom expression and relative virus concentration in potato plants infected with potato virus YN and YO

Strain-specific hypersensitive (HR) and extreme resistance (ER) phenotypes elicited in potato plants by three Potato virus Y (PVY) isolates in strain groups PVYO (BL and DEL3) and PVYD (KIP1) were studied. PVYO and PVYD isolates elicit HR genes Ny or putative Nd, respectively, and all three isolates elicit ER gene Ry. They were inoculated to 39 Australasian, European, or North American potato cultivars released over a 117-year period and harvested tubers were replanted. Both primary and secondary symptoms were recorded. Two European cultivars always developed ER following sap and graft inoculation and, thus, carried comprehensive PVY resistance gene Ry. One Australasian and two European cultivars always developed susceptible phenotypes and, thus, lacked genes Ry, Ny, and putative Nd. Sap inoculation with isolate KIP1 elicited localized HR (LHR) in 31 cultivars and both LHR and systemic HR (SHR) in three others; thus, all carried putative Nd. Isolates BL and DEL3 both elicited susceptible phenotypes in 11 of these 34 cultivars but LHR alone, SHR alone, or both LHR and SHR in the other 23 which, therefore, all carry Ny. With these two isolates, SHR expression ranged from very severe to very weak, with the greatest numbers of isolate–cultivar combinations occurring in the severe category with BL (n = 11) and moderate category (n = 12) with DEL3. Within the same isolate–cultivar combination, overall, SHR symptom expression was weaker with secondary than primary infection. With both primary and secondary infection, SHR expression was most severe with KIP1 and weakest with DEL3. Genes Ny and putative Nd were present in cultivars released between 1939 and 2010 or 1893 and 2010, respectively, occurring in cultivars from all three world regions. These findings have important implications concerning breeding new PVY-resistant potato cultivars, especially for countries lacking healthy seed potato stocks, or where subsistence farmers cannot afford them. An alternative to including gene Ry is incorporating as many strain-specific PVY resistance genes as possible.

Download Full-text

Effect of temperature on sweet potato virus disease symptom expression

African Journal of Agricultural Research ◽

10.5897/ajar2017.12442 ◽

2017 ◽

Vol 12 (27) ◽

pp. 2295-2309 ◽

Cited By ~ 2

Author(s):

Sseremba Godfrey ◽

Mukasa Settumba ◽

Kyamanywa Samuel

Keyword(s):

Sweet Potato ◽

Potato Virus ◽

Virus Disease ◽

Disease Symptom ◽

Effect Of Temperature ◽

Symptom Expression ◽

Potato Virus Disease ◽

Sweet Potato Virus ◽

Sweet Potato Virus Disease

Download Full-text

CHARACTERISTICS OF VIRAL INFECTION DEVELOPMENT CAUSED BY POTATO VIRUS M UNDER THE IMPACT OF BIOPREPARATIONS ON POTATO PLANTS

Agriciltural microbiology ◽

10.35868/1997-3004.20.60-65 ◽

2014 ◽

Vol 20 ◽

pp. 60-65

Author(s):

I. H. Budzanivska ◽

T. O. Bova ◽

O. O. Kucheriavenko ◽

O. V. Pyrih ◽

O. O. Dmytruk

Keyword(s):

Viral Infection ◽

Potato Virus ◽

Higher Plants ◽

Virus Concentration ◽

Developmental Study ◽

Total Chlorophyll ◽

Potato Plants ◽

Total Chlorophyll Content ◽

Potato Virus M ◽

The Impact

The paper presents the results of developmental study of viral infection caused by Potato virus M under the impact of microbial preparations on potato plants. Use of biopreparations Biogran and Bactopaslen, at artificial plants infection with PVM had ensured development of higher plants infected with virus, increase of total chlorophyll content compared to control, decrease of virus concentration in plants and activity of ribonuclease enzyme in potato plants infected with PVM.

Download Full-text

Cytological responses of susceptible and extremely resistant potato plants to inoculation with potato virus Y

Physiological and Molecular Plant Pathology ◽

10.1006/pmpp.1999.0216 ◽

1999 ◽

Vol 55 (3) ◽

pp. 143-150 ◽

Cited By ~ 32

Author(s):

J. HINRICHS-BERGER ◽

M. HARFOLD ◽

S. BERGER ◽

H. BUCHENAUER

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Potato Plants

Download Full-text

Detection and genome characterization of Potato virus Y isolates infecting potato (Solanum tuberosum L.) in La Union (Antioquia, Colombia)

Agronomía Colombiana ◽

10.15446/agron.colomb.v34n3.59014 ◽

2016 ◽

Vol 34 (3) ◽

pp. 317-328 ◽

Cited By ~ 4

Author(s):

Pablo Gutiérrez S. ◽

Mauricio Marín M. ◽

Daniel Muñoz E.

Keyword(s):

Solanum Tuberosum ◽

Potato Virus ◽

Potato Virus Y ◽

Seed Tuber ◽

Rt Pcr ◽

Potato Plants ◽

Nextgeneration Sequencing ◽

Prevalent Strain ◽

Detailed Molecular Analysis

Potato virus Y (PVY) is one of the most severe viruses affecting the production of potato (Solanum tuberosum) in the world. This study presents a detailed molecular analysis using nextgeneration sequencing (NGS), IC-RT-qPCR and RT-PCR on the PVY isolates infecting seed-tubers and foliage of potato plants cv. Diacol-Capiro in La Union (Antioquia, Colombia). Analysis of incidence by IC-RT-qPCR in 15 random leaf samples of three cultivation plots and fifteen sprouting tuber eye-buds reveal infection levels between 13.4 and 80%; a higher incidence of 86.7% was observed in seed-tuber samples with threshold cycle (Ct) values as low as 24.3. Genome assembly from a bulk of foliage samples resulted in a consensus PVY genome (PVY_LaUnionF) of 9,702 nt and 399 polymorphic sites within the polyprotein ORF; while the assembled genome from sprouts of tubers has 9,704 nt (PVY_LaUnionT) and contained only six polymorphic nucleotide sites. Phylogenetic analysis demonstrates that the PVY isolates from leaf samples are in the recombinant PVYNTN group (sequence identity >99%); while those from tuber sprouts are in the PVYN/NTN group with identities above 95%. Sanger sequencing of viral capsid suggests the presence of a third variant related to PVYO, a prevalent strain reported in potato fields worldwide.

Download Full-text

Effect of Potato virus Y on Yield of Three Potato Cultivars Grown Under Different Nitrogen Levels

Plant Disease ◽

10.1094/pd-90-0073 ◽

2006 ◽

Vol 90 (1) ◽

pp. 73-76 ◽

Cited By ~ 39

Author(s):

Jonathan L. Whitworth ◽

Phil Nolte ◽

Chris McIntosh ◽

Robert Davidson

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Total Yield ◽

Russet Burbank ◽

Yield Reduction ◽

Symptom Expression ◽

Russet Norkotah ◽

Nitrogen Levels ◽

Foliar Symptoms ◽

Significant Yield

Previous studies have shown that Potato virus Y (PVY) reduces yield in many cultivars. Typical foliar symptoms can include veinal necrosis, leaf drop, and a mosaic pattern sometimes accompanied by leaf roughness. Infection by PVY in Russet Burbank produces identifiable PVY symptoms, whereas cv. Russet Norkotah expresses mild, almost latent symptoms. Yield also is influenced by nitrogen fertilizer levels. This research was conducted to determine whether increased nitrogen mitigates yield reduction caused by PVY. Russet Norkotah, CO80011-5, and Russet Burbank were used in replicated plots of non-PVY-infected and PVY-infected plants at three nitrogen levels in 1995 and 1996. There was a significant yield reduction between PVY-negative and PVY-positive plots in all cultivars, at most nitrogen levels. PVY yield reduction was similar (approximately 38%) in the mild symptom expression clones of Russet Norkotah and CO80011-5, whereas the yield reduction in Russet Burbank, which exhibits typical symptom expression, was 63.5%. We conclude that increased nitrogen can influence total yield, but does not significantly mitigate the yield reduction due to PVY infection.

Download Full-text

Respiration of tobacco leaves infected with different strains of potato virus X

Canadian Journal of Botany ◽

10.1139/b69-103 ◽

1969 ◽

Vol 47 (5) ◽

pp. 723-730 ◽

Cited By ~ 3

Author(s):

M. M. Dwurazna ◽

M. Weintraub

Keyword(s):

Potato Virus ◽

Great Increase ◽

Experimental Period ◽

Potato Virus X ◽

Metabolic Inhibitors ◽

Virus Concentration ◽

Tobacco Leaves ◽

Respiration Rates ◽

Local Lesions ◽

Different Strains

A study was made of the effects on respiration by four strains of potato virus X varying in the severity of symptoms they produce on tobacco leaves. No effects on respiration rates were observed in either inoculated or systemically infected but symptomless leaves, even though there was a significant increase in virus concentration during the experimental period. However, a significant increase was detected in any leaves on which symptoms developed, this increase being directly correlated with the severity of symptom expression. At the same time a great increase in virus concentration was found. Measurement of respiration patterns of single local lesions showed that an increase in respiration occurred each time a chlorotic and necrotic ring formed in the tissues. The use of metabolic inhibitors indicated that metabolic controls are different in the leaves infected with latent or mild strains, as compared to those infected with the severe, necrotizing strains.

Download Full-text