Separate and Combined Effects of Chlortetracycline and Oxytetracycline on Growth and Antioxidant Enzymes in Myriophyllum Aquaticum

As two typical tetracyclines, chlortetracycline (CTC) and oxytetracycline (OTC) coexist widely in water. In the experiment, Myriophyllum aquaticum (Vell.) Verdc was exposed to the orthogonal hydroponic environment with the concentrations of CTC and OTC at 1, 10, and 50 mg/L for 7 days (7 D) and 14 days (14 D). The results showed that the plant height, relative growth rates (RGR), and photosynthetic pigment contents of M. aquaticum decreased significantly after 14 D of CTC/ OTC, which was stronger than that after 7 D, and the toxicity of CTC was stronger than OTC. The combined toxicity of the two on the RGR and total chlorophyll content was mainly synergistic or additive after 7 D. After 14 D, the interaction changed to antagonism or addition, and the response of total chlorophyll content was more obvious than that of RGR. After 7 D of combined treatments, the malondialdehyde (MDA) content decreased significantly, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities responded to different combined concentrations, respectively, and CAT was the main protective enzyme of M. aquaticum. After 14 D of combined treatments, the hydrogen peroxide (H2O2) content increased significantly, the MDA content increased first and then decreased, and POD was the main protective enzyme among the three antioxidant enzymes. Overall, this study provided the first insight into the single and combined toxicity of CTC and OTC on the growth and physiology of M. aquaticum at different time periods, revealing the reversal of the combined toxicity with time of exposure.

Interactive Impact of Biochar and Arbuscular Mycorrhizal on Root Morphology, Physiological Properties of Fenugreek (Trigonella foenum-graecum L.) and Soil Enzymatic Activities

Arbuscular mycorrhizal fungi (AMF) inoculation and biochar amendment has been reported to improve the growth of several crop plant; however, their role in stress amelioration individually as well as in combination has not been worked out. Limited information is available about the synergistic use of biochar and Arbuscular Mycorrhizal Fungi (AMF). Here, we investigated the synergistic effect of biochar and AMF on plant development, root architecture, the physiological performance of fenugreek (Trigonella foenum-graecum), and soil enzymatic activities. Biochar and AMF were shown to have a considerable effect on plant height, according to the data (53.3 and 66.6%, respectively), leaf number (22.5 and 45.1%), total root length (19.8 and 40.1%), root volume (32.1 and 71.4%), chlorophyll a content (26.0 and 17.8%), chlorophyll b content (50.0 and 28.9%), total chlorophyll content (30.0 and 18.1%), and carotenoid content (60.0 and 48.0%) over the control treatment. There was a considerable increase in plant height when biochar and AMF were combined together by 80.9%, total root length by 68.9%, projected area by 48.7%, root surface area by 34.4%, root volume by 78.5%, chlorophyll a content by 34.2%, chlorophyll b content by 68.4%, total chlorophyll content by 44.5%, and carotenoid content by 84.0% compared to the control. Our results recommend that the combination of biochar and AMF is advantageous in fenugreek growth, microbial biomass, and soil enzyme activities.

Effect of Some Nutritional Factors on The Growth and Yield of Apple cv. Ibrahimi

The field experiment was carried out during the planting season of 2020 in one of the private sector orchards in the Saqlawiya sub-district of Fallujah district to study the effect of foliar spraying with zinc, boron and potassium on some vegetative growth traits and the yield of apples of CV. Ibrahimi, the first factor is represented by spraying with boric acid H3BO3 at a Concentrations of (0, 1) g. L-1, mentioned as B0, B1, and the second factor consisted of spraying with zinc sulfate ZnSO4 at a concentrations of( 0, 3) g. L-1 mentioned as Z0, Z1 and the third factor is spraying with potassium sulfate K2SO4 at concentrations (0, 2.5, 5) g. L-1 mentioned asK0-K1, K2 respectively. The results showed that the effect of spraying with the study factors led to a significant increase in most of the studied traits represented by (the area of one leaf, the total chlorophyll content of the leaves, the percentage of carbohydrates in the branches, the percentage of fallen fruits, the yield of one tree, the average weight of the fruit, the percentage of total sugars in the fruits, and the content of the fruits of vitamin C) reached ( 27.81 cm2, 82.67mg100g-1, 14.37%, 30.74%, 27.98kg.tree-1, 45.13g, 8.57%, 4.87 mg100g-1) sequentially.

Response of Two variety of Snake Cucumber to Spray with Atonics Biostimulator in Some Chemical Parameters

The experiment was carried out in unheated greenhouses in the station of the College of Agriculture and Marshes/Dhi Qar University during the fall season 2020-2021, to study the effect of atonic biostimulant in chemical parameters growth and yield of snak cucumber (Cucumis melo. Var flexuosus naud) under protected cultivation,. The experiment included two factors: First one two cultivars of cucumber (Al-Baghdadi and Al-Halawi), second factor was atonic biostimulant (0, 0.5, 1, 1.5 ml L−1) A Randomized Complete Block Design (R.C.B.D.) were used with three replicates and means were compared according to Least Significant Difference Test (L.S.D.)test at probability of 0.05. Determining the stages of plant growth in which the foliar spraying process was carried out and the number of sprays. Results were summarized as follows: Al-Baghdadi cultivar was significantly superior in (content of total soluble carbohydrates in fruits, vitamin (C) and total chlorophyll content of fruits), reached (6.181, 101.8 and 9,744) mg 100 g−1 respectively Compare with Al-Halawi cultivar which recorded the highest rate of protein in fruits(4.115 %.), The addition of atonic spray treatment at a concentration 1.5 ml L−1 was significantly in (content of total soluble carbohydrates in fruits, vitamin (C), total chlorophyll and proteins in fruits), which recorded (7.327 mg 100g−1 and 132.5 mg 100g−1 and 10,860 mg 100g−1 and 4.323%) respectively, but the control treatment gave the lowest rates. the interaction between Al-Baghdadi cultivar and 1.5 mL−1 atonic biostimulant was superior (content of total soluble carbohydrates, vitamin (C), total chlorophyll content in fruits), 145.0 mg 100 g−1, 11.019 mg 100 g−1 and 4.354%) respectively, but the comparison treatment gave the lowest rates.

Leaf Color Changes and Photosynthetic Characteristics of Five Superior Late-deciduous Ginkgo biloba Cultivars

Ginkgo biloba, a relict plant, has been popularized and planted in most areas of China for its leaves, timber, and fruits. In the present study, the dynamic changes in leaf color, leaf pigment content during the color change period, and photosynthetic characteristics in different growth periods were studied to explore the coloring mechanism and adaptability of five late-deciduous superior Beijing G. biloba cultivars (LD1–LD5). The results showed that the leaf color change of each superior cultivar was relatively stable, and the discoloration period of LD3 and LD5 was later than that of others. From September to November, the chlorophyll a, chlorophyll b, and total chlorophyll content in all superior cultivars showed a downward trend, except in LD3, in which the pigment content was slightly higher in October than in September. Except in LD3 and LD4, the ratio of carotene content to total chlorophyll content in other cultivars slightly decreased in October. In May, the photosynthetic capacity of LD5 was stronger than that of other cultivars. The photosynthetic capacity of LD3 was strong in July and October. Our results imply that LD3 and LD5 are suitable for mixed planting with common G. biloba to increase the overall leaf color viewing period. Ginkgo biloba leaves turn yellow in autumn because of both a decrease in the chlorophyll content after leaf senescence and an increase in the Car content during leaf senescence. Although LD5 presented rapid seedling emergence, LD3 grew faster during the vigorous and late growth stages and is thus suitable for agricultural production.

Effect of IBA, NAA and their Combination on Rooting and Biochemical Parameters of Stem Cuttings in Barbados Cherry

An investigation was to assess the influence of growth regulators IBA, NAA and their combination on rooting and biochemical parameters of stem cuttings in Barbados cherry. It was observed that, the least number of days (21.33) taken for root initiation, maximum percentage of rooting (80.00) was observed in cutting treated with IBA 5000 ppm followed by IBA 4500 ppm over other treatments. Significantly highest Chlorophyll-a (1.26), Chlorophyll –b (0.55) and total chlorophyll content (1.82 mg/g of fresh weight) of leaves and C : N ratio (8.74) was observed in cuttings treated with IBA 5000 ppm followed by IBA 4500 ppm over all other treatments.

Application of Led on Plant Growth Unit in Tomato Cultivation

Effects of Light Emitting Diode (LED) lamps on plant growth with different modulations on the tomato plant were investigated by applying under actual conditions in the greenhouses. Thus, four different pulse width modulation (PWM) systems from red and blue LEDS at different wavelengths were designed. Trials were performed by using direct current (Mode1), 5Khz-φ = 0o (Mode2), 5Khz-φ = 180o (Mode3) and 1Khz-φ = 0o (Mode4) and the tomato grown under the sun at the same conditions were compared for controlling purposes. Stomata movements and total chlorophyll content of the tomatoes were examined, and the results were compared to the tomatoes that were grown under sun. The custom developed artificial four light source system, a treatment of Mode 2 produced the highest yield, while each treatment played its special role in regulating plant morphology and physiology. Bangladesh J. Bot. 50(3): 523-529, 2021 (September)

Heat stress-induced changes in shoot and root characteristics of genotypes of tepary bean (Phaseolus acutifolius A. Gray), common bean (Phaseolus vulgaris L.) and their interspecific lines

Heat stress is a major limitation to grain yield in common bean (Phaseolus vulgaris L.). Tepary bean (Phaseolus acutifolius A. Gray) is better adapted to heat stress than common bean. Ten tepary bean accessions, four common bean genotypes and four interspecific lines involving P. vulgaris and P. acutufolius, P. coccineus and P. dumosus were evaluated for tolerance to heat stress conditions induced under greenhouse conditions and these were compared to plants grown under ambient temperatures. The high temperature treatment was 29 ±5 °C during the day and was >24 °C (up to 27 °C) during the night, while the ambient temperature (AT) treatment was 25 ±5 °C during the day and 19± 2 °C at night. The genotypic differences were evaluated for morpho-physiological characteristics of shoot and root and also yield components. The Genotype and Genotype × Temperature interactions were significant for all shoot and root morpho-physiological characteristics evaluated. Higher temperature (HT) significantly affected leaf photosynthetic efficiency, total chlorophyll content, and stomatal conductance. The effect was positive or negative, depending on the genotypes. Tepary accessions showed reduced total chlorophyll content, while common bean genotypes and the interspecific lines were less affected. Tepary accessions also showed reduced stomatal conductance, but increased leaf photosynthetic efficiency under HT. Common bean genotypes increased stomatal conductance and decreased leaf photosynthetic efficiency. High temperature decreased total root length, specific root length and pod biomass compared to ambient conditions, but there was no marked effect on pollen viability of the tested genotypes. The superior adaptation of tepary germplasm accessions to high temperature is attributed to their ability to regulate stomatal opening and photosynthetic efficiency, together with a superior ability to remobilize photosynthates from older leaves to pods during physiological maturity

Evaluation of Early Stage Traits as an Indicator of Genetic Variation in Winter Lentil

Lentil is one of the oldest cultured crops with cereals which are cultivated in the wide geography of the world due to its high adaptability, quality nutritional value, important usage areas and economic value. Morphological, physiological and biochemical parameters during the early vegetative stage are an important indicator of adaptation to environmental conditions. This study was carried out to investigate the variation on some agronomic and physiological traits of 49 lentil genotypes during the early growth stage. Although there were significant differences in terms of plant height and total chlorophyll content, differences between genotypes for plant dry and fresh weight, stem diameter and length of epicotyl were statistically found insignificant. Plant height, plant fresh weight, plant dry weight, length of epicotyl, stem diameter and total chlorophyll content varied between 2.53-4.78 cm, 0.17-0.39 g, 0.04-0.13 g, 1.53-3.00 cm, 0.72-1.05 cm and 28.4-38.2%, respectively. As a result, it was determined that observations taken 30 days after emergence are not sufficient to constitute genetic variation and use as an early prediction criteria field conditions. The longer-term and comprehensive studies should be carried out to form genetic variation and interpret the results in field conditions.

Utilization Efficiency of Growth Regulators in Wheat under Drought Stress and Sandy Soil Conditions

Drought stress and nutrient status are highly important for plant growth and productivity. Two field experiments were conducted during two consecutive seasons (2017–2018 and 2018–2019) at El-Molak, Abo-Hammad, Sharkia, Egypt. This work was conducted under sandy soil conditions to evaluate the effects of foliar application with growth regulators (PGRs) such as cycocel (CCC), applied at 0, 500, or 1000 mg L−1, and/or salicylic acid (SA), applied at 0, 0.05, or 0.1 mM on the productivity as well as improving drought tolerance of three wheat cultivars, i.e., Gemmeiza 11, Misr 1, and Giza 171 under three irrigation intervals, i.e., 10, 15, and 20 days. Foliar spray was given at 35 and 50 days after planting (DAP). The obtained results showed that mean squares as a result of the main effect and first- and second-order interactions were significant (p ≤ 0.01) for all studied traits. The application of SA increased total chlorophyll content and flag leaf area (cm2) while the number of days to 50% heading was decreased; however, the number of spikes m−2, protein and proline contents were increased with the application of CCC. The cultivar Misr 1 outperformed the other cultivars in the most studied traits. Estimates of heritability in the broad sense (h2b) were, on average, higher in five physiological traits than other agronomic traits, and the highest estimate of h2b (95.1%) was shown by the number of days to 50% heading followed by protein content (91.90%). Among the interactions between irrigation and growth regulators, the I(10) × SA(0.1) recorded the highest flag leaf area (cm2), SPAD value, number of grains spike−1, 1000-grain weight (g), and grain yield (t ha−1). Among the interactions between irrigation and cultivars, the I(10) × Misr 1 recorded the highest flag leaf area (cm2), SPAD value, number of grains spike−1, and grain yield (t ha−1). Among the interactions among irrigation, growth regulators and cultivars, the I(10) × SA(0.1) × Misr 1 recorded the highest flag leaf area (cm2), number of grains spike−1, 1000-grain weight (g), and grain yield (t ha−1). Correlation coefficient between grain yield (t ha−1) and each of the number of days to 50% heading, flag leaf area, total chlorophyll content, number of spikes m−2, number of grains spike−1, and 1000-grain weight was positive and significant. Three main factors for the studied variables were created from the application of the factor analysis technique. Grain yield ha−1 (Y) can be predicted by the method of forwarding stepwise through applying the automatic linear regression analysis. Besides, the best prediction equation of grain yield ha−1 (Y) was formulated as: Ỷ = −14.36 + 0.11 number of grains spike−1 (NGS) + 0.09 1000-grain weight (THW) + 0.04 number of spike m−2 (NSm) + 0.03 days to 50% heading (DF) + 0.02 total chlorophyll content (TC) with adjusted-R2 (87.33%).