Role of the methionine cycle in the temperature‐sensitive responses of potato plants to potato virus Y

The complexity of the Potato virus Y (PVY) (Potyviridae: Potyvirus) pathosystem is affected by the presence of several virus strains that differ in their ability to produce tuber necrosis and by the presence of an alternate host that could increase the amount of inoculum in potato fields. Solanum sarrachoides (Sendtner) is an invasive weed from South America present in Pacific Northwest potato agro-ecosystems. It serves as reservoir of PVY and its most efficient vectors: the green peach aphid, Myzus persicae (Sulzer), and the potato aphid, Macrosiphum euphorbiae (Thomas). The role of S. sarracoides as vector and virus reservoir in PVY epidemiology was investigated through a series of laboratory and greenhouse experiments. We studied the symptoms produced in S. sarracoides upon infection with necrotic and non-necrotic strains of PVY and looked at the percentage of infection and titer accumulation of these strains. PVY infection in S. sarrachoides produced symptoms similar to those produced in PVY-infected potato plants. Mottling and yellowing were the main symptoms of infection observed in S. sarrachoides plants, especially by PVYO and PVYNTN infection. Greenhouse transmission studies revealed that PVY-infected S. sarrachoides increased the transmission rate of PVY necrotic strains by M. persicae. The necrotic strain PVYNTN reached higher titer in S. sarrachoides than in potato plants when compared to PVYO and PVYN:O These findings have broadened our understanding of the role and importance of S. sarrachoides in the PVY epidemiology in the potato ecosystems and could potentially be included in the development or optimization of virus management programs. Accepted for publication 15 March 2010. Published 26 May 2010.

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The Resistance Responses of Potato Plants to Potato Virus Y Are Associated with an Increased Cellular Methionine Content and an Altered SAM:SAH Methylation Index

Viruses ◽

10.3390/v13060955 ◽

2021 ◽

Vol 13 (6) ◽

pp. 955

Author(s):

Nadezhda Spechenkova ◽

Igor A. Fesenko ◽

Anna Mamaeva ◽

Tatyana P. Suprunova ◽

Natalia O. Kalinina ◽

...

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Potato Cultivar ◽

Potato Plants ◽

Methylation Index ◽

Different Temperatures ◽

Adenosyl Methionine ◽

Methionine Cycle ◽

Virus Interactions ◽

Methionine Content

Plant-virus interactions are frequently influenced by elevated temperature, which often increases susceptibility to a virus, a scenario described for potato cultivar Chicago infected with potato virus Y (PVY). In contrast, other potato cultivars such as Gala may have similar resistances to PVY at both normal (22 °C) and high (28 °C) temperatures. To elucidate the mechanisms of temperature-independent antivirus resistance in potato, we analysed responses of Gala plants to PVY at different temperatures using proteomic, transcriptional and metabolic approaches. Here we show that in Gala, PVY infection generally upregulates the accumulation of major enzymes associated with the methionine cycle (MTC) independently of temperature, but that temperature (22° C or 28° C) may finely regulate what classes accumulate. The different sets of MTC-related enzymes that are up-regulated at 22 °C or 28 °C likely account for the significantly increased accumulation of S-adenosyl methionine (SAM), a key component of MTC which acts as a universal methyl donor in methylation reactions. In contrast to this, we found that in cultivar Chicago, SAM levels were significantly reduced which correlated with the enhanced susceptibility to PVY at high temperature. Collectively, these data suggest that MTC and its major transmethylation function determines resistance or susceptibility to PVY.

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Cytological responses of susceptible and extremely resistant potato plants to inoculation with potato virus Y

Physiological and Molecular Plant Pathology ◽

10.1006/pmpp.1999.0216 ◽

1999 ◽

Vol 55 (3) ◽

pp. 143-150 ◽

Cited By ~ 32

Author(s):

J. HINRICHS-BERGER ◽

M. HARFOLD ◽

S. BERGER ◽

H. BUCHENAUER

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Potato Plants

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Detection and genome characterization of Potato virus Y isolates infecting potato (Solanum tuberosum L.) in La Union (Antioquia, Colombia)

Agronomía Colombiana ◽

10.15446/agron.colomb.v34n3.59014 ◽

2016 ◽

Vol 34 (3) ◽

pp. 317-328 ◽

Cited By ~ 4

Author(s):

Pablo Gutiérrez S. ◽

Mauricio Marín M. ◽

Daniel Muñoz E.

Keyword(s):

Solanum Tuberosum ◽

Potato Virus ◽

Potato Virus Y ◽

Seed Tuber ◽

Rt Pcr ◽

Potato Plants ◽

Nextgeneration Sequencing ◽

Prevalent Strain ◽

Detailed Molecular Analysis

Potato virus Y (PVY) is one of the most severe viruses affecting the production of potato (Solanum tuberosum) in the world. This study presents a detailed molecular analysis using nextgeneration sequencing (NGS), IC-RT-qPCR and RT-PCR on the PVY isolates infecting seed-tubers and foliage of potato plants cv. Diacol-Capiro in La Union (Antioquia, Colombia). Analysis of incidence by IC-RT-qPCR in 15 random leaf samples of three cultivation plots and fifteen sprouting tuber eye-buds reveal infection levels between 13.4 and 80%; a higher incidence of 86.7% was observed in seed-tuber samples with threshold cycle (Ct) values as low as 24.3. Genome assembly from a bulk of foliage samples resulted in a consensus PVY genome (PVY_LaUnionF) of 9,702 nt and 399 polymorphic sites within the polyprotein ORF; while the assembled genome from sprouts of tubers has 9,704 nt (PVY_LaUnionT) and contained only six polymorphic nucleotide sites. Phylogenetic analysis demonstrates that the PVY isolates from leaf samples are in the recombinant PVYNTN group (sequence identity >99%); while those from tuber sprouts are in the PVYN/NTN group with identities above 95%. Sanger sequencing of viral capsid suggests the presence of a third variant related to PVYO, a prevalent strain reported in potato fields worldwide.

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Mutations in Potato virus Y Genome-Linked Protein Determine Virulence Toward Recessive Resistances in Capsicum annuum and Lycopersicon hirsutum

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.2004.17.3.322 ◽

2004 ◽

Vol 17 (3) ◽

pp. 322-329 ◽

Cited By ~ 145

Author(s):

Benoît Moury ◽

Caroline Morel ◽

Elisabeth Johansen ◽

Laurent Guilbaud ◽

Sylvie Souche ◽

...

Keyword(s):

Amino Acid ◽

Capsicum Annuum ◽

Potato Virus ◽

Potato Virus Y ◽

Cdna Clones ◽

Lycopersicon Hirsutum ◽

Potato Virus A ◽

Single Nucleotide Change ◽

Protein Encoding

The recessive resistance genes pot-1 and pvr2 in Lycopersicon hirsutum and Capsicum annuum, respectively, control Potato virus Y (PVY) accumulation in the inoculated leaves. Infectious cDNA molecules from two PVY isolates differing in their virulence toward these resistances were obtained using two different strategies. Chimeras constructed with these cDNA clones showed that a single nucleotide change corresponding to an amino acid substitution (Arg119His) in the central part of the viral protein genome-linked (VPg) was involved in virulence toward the pot-1 resistance. On the other hand, 15 nucleotide changes corresponding to five putative amino acid differences in the same region of the VPg affected virulence toward the pvr21 and pvr22 resistances. Substitution models identified six and five codons within the central and C terminal parts of the VPg for PVY and for the related potyvirus Potato virus A, respectively, which undergo positive selection. This suggests that the role of the VPg-encoding region is determined by the protein and not by the viral RNA apart from its protein-encoding capacity.

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