Electron microscope observations on plastid division in root meristematic cells ofPisum sativum L.

1975 ◽  
Vol 88 (4) ◽  
pp. 319-321 ◽  
Author(s):  
Ken-ichi Suzuki ◽  
Rikizo Ueda
Keyword(s):  
Electron Microscope ◽  
Plastid Division ◽  
Meristematic Cells ◽  
Root Meristematic Cells

Sites of Protein Synthesis in Nucleoli of Root Meristematic Cells of Allium Cepa As Shown By Radioautography With [3H]Arginine

1967 ◽  
Vol 2 (4) ◽  
pp. 473-480
Author(s):  
L. A. CHOUINARD ◽  
C. P. LEBLOND
Keyword(s):  
Protein Synthesis ◽  
Electron Microscope ◽  
Allium Cepa ◽  
Chase Period ◽  
Meristematic Cells ◽  
Root Meristematic Cells ◽  
Silver Grains

The interphase nucleolus in root meristematic cells of Allium cepa may be divided into four regions, three of which are always present: the fibrillar, granular and lacunar regions, while the fourth or vacuolar region may be missing. The sites of protein synthesis in nucleoli were investigated by means of light and electron-microscope radioautography after a 5-min immersion of the roots in a solution of [3H]arginine. The radioautographs of interphase nucleoli showed many silver grains over both the fibrillar and the granular regions. Occasional silver grains were also recorded over, or close to, the lacunar regions, but none were over the vacuolar regions. A 15-min chase period did not change the radioautographic pattern. It is concluded that the three permanent regions of the interphase nucleoli, namely the fibrillar, the granular and the DNA-containing lacunar regions, are sites of protein synthesis.

scholarly journals Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L.

2015 ◽  
Vol 37 (1) ◽  
pp. 101 ◽  
Author(s):  
Gleuvânia Santana Marques ◽  
Josefa Janaína do Anjos Sousa ◽  
Ana Paula Peron
Keyword(s):  
Allium Cepa ◽  
Meristematic Cells ◽  
Food Dye ◽  
Root Meristematic Cells ◽  
Ponceau 4R

scholarly journals Sodium cacodylate as antimitotic agent

2014 ◽  
Vol 57 (3) ◽  
pp. 329-340
Author(s):  
Jadwiga A. Tarkowska
Keyword(s):  
Allium Cepa ◽  
Mitotic Spindle ◽  
Sodium Cacodylate ◽  
Antimitotic Agent ◽  
Ultrastructural Studies ◽  
Meristematic Cells ◽  
Cacodylate Buffer ◽  
Root Meristematic Cells ◽  
Dividing Cells

The effect of pure sodium cacodylate on dividing cells was studied. The root meristematic cells of <em>Allium cepa</em> L. (the roots were squashed in acetoorcein) and endosperm cells of <em>Haemanthus katherinae</em> Bak. (<em>in vitro</em> observations) were used. Serious disturbances in karyokinesis and cytokinesis were found that led most often to the formation of polyploid or multinucleate (<em>A. cepa</em>) cells. These results point to damage of the mitotic spindle and phragmoplast. Careful use of cacodylate buffer in ultrastructural studies of microtubules is advised.

scholarly journals Functional alterations of root meristematic cells of Arabidopsis thaliana induced by a simulated microgravity environment

2016 ◽  
Vol 207 ◽  
pp. 30-41 ◽  
Author(s):  
Elodie Boucheron-Dubuisson ◽  
Ana I. Manzano ◽  
Isabel Le Disquet ◽  
Isabel Matía ◽  
Julio Sáez-Vasquez ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Simulated Microgravity ◽  
Microgravity Environment ◽  
Meristematic Cells ◽  
Root Meristematic Cells

The subcellular concentration of ions and elements in thin cryosections of onion root meristem cells. An electron-probe EDS study

1984 ◽  
Vol 72 (1) ◽  
pp. 295-306
Author(s):  
I.L. Cameron ◽  
K.E. Hunter ◽  
N.K. Smith
Keyword(s):  
Electron Probe ◽  
Dry Weight ◽  
Cell Types ◽  
Meristematic Cells ◽  
Freeze Dried ◽  
Concentration Of Ions ◽  
Intracellular Compartments ◽  
Root Meristematic Cells ◽  
Storage Granules ◽  
First Time

Quantitative electron-probe energy dispersive X-ray microanalysis has, for the first time, been accomplished at a subcellular level in plant tissue using cryofixed and thin freeze-dried cryosections. The subcellular concentrations of Na+, Cl-, K+, P, S, Mg2+ and Ca2+ were measured in mol/kg dry weight in two types of root meristematic cells of the onion, Allium cepa. The cell wall of the meristematic cells had much higher concentrations of K+ and Ca2+ than was found in the intracellular compartments. Storage granules in the protoderm cells were about 6–12 times lower in P and were about four times higher in S as compared to other intracellular compartments. Comparison between the concentrations of ions and other elements in meristematic plant cells and in mouse cardiac myocytes confirms that major differences in cytoplasmic Na+ and Cl- concentrations do indeed exist between these cell types.

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