Cytogenetic analysis of gallbladder neoplasms using fluorescence in situ hybridization (FISH)

1997 ◽  
Vol 4 (4) ◽  
pp. 431-435
Author(s):  
Atsushi Nanashima ◽  
Hiroyuki Yamaguchi ◽  
Shinichi Shibasaki ◽  
Juan-Eiki Nishizawa-Takano ◽  
Takashi Tsuji ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cytogenetic Analysis ◽  
Gallbladder Neoplasms

scholarly journals Detection of trisomy 12 in chronic lymphocytic leukemia by fluorescence in situ hybridization to interphase cells: a simple and sensitive method

Blood ◽  
1992 ◽  
Vol 79 (7) ◽  
pp. 1796-1801 ◽  
Author(s):  
J Anastasi ◽  
MM Le Beau ◽  
JW Vardiman ◽  
AA Fernald ◽  
RA Larson ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Chronic Lymphocytic Leukemia ◽  
Fluorescence In Situ Hybridization ◽  
Cytogenetic Analysis ◽  
Normal Karyotype ◽  
Lymphocytic Leukemia ◽  
Chromosome 12 ◽  
Interphase Cells ◽  
Trisomy 12

Abstract Trisomy 12 is the most common cytogenetic abnormality in chronic lymphocytic leukemia (CLL), and a number of studies have suggested that it may be an adverse prognostic indicator. We have evaluated the usefulness of fluorescence in situ hybridization with a chromosome 12- specific probe as a simple means for detecting trisomy 12 in interphase cells. Forty cases of B-cell CLL previously studied with conventional cytogenetic techniques were analyzed with a biotinylated probe to the centromeric region of chromosome 12. Thirty of these retrospective cases could be reevaluated with in situ hybridization. Our analysis showed three hybridization signals (ie, trisomy 12) in interphase cells from seven of seven cases found previously to have trisomy 12. Trisomy 12 was also detected in five additional cases: in one case thought to have a normal karyotype, in two cases that had been inadequate for routine cytogenetic analysis, and in two cases that had been found to have an abnormal karyotype without trisomy 12. In a prospective series of 20 newly accrued CLL cases, all cases were analyzed successfully by in situ hybridization and six (30%) showed trisomy 12. We were able to perform the analysis on routinely prepared and previously Wright- stained peripheral blood smears. We conclude that fluorescence in situ hybridization is a simple means for the detection of trisomy 12 in CLL. The technique is more sensitive than conventional cytogenetic analysis and would be a useful tool in clinical studies.

Application of multiplex fluorescence in situ hybridization in the cytogenetic analysis of primary gastric carcinoma

2002 ◽  
Vol 135 (1) ◽  
pp. 23-27 ◽  
Author(s):  
Maria I. Stamouli ◽  
Angeliki D. Ferti ◽  
Anna D. Panani ◽  
John Raftakis ◽  
Carla Consoli ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Gastric Carcinoma ◽  
Fluorescence In Situ Hybridization ◽  
Cytogenetic Analysis ◽  
Primary Gastric Carcinoma

scholarly journals Quick-FISH: A Rapid Fluorescence In Situ Hybridization Technique for Molecular Cytogenetic Analysis

BioTechniques ◽  
1998 ◽  
Vol 24 (5) ◽  
pp. 826-830 ◽  
Author(s):  
Sushanta K. Banerjee ◽  
Allan P. Weston ◽  
Diane L. Persons ◽  
Donald R. Campbell
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cytogenetic Analysis ◽  
Hybridization Technique ◽  
Molecular Cytogenetic

Effect of Fixation on Interphase Cytogenetic Analysis by Direct Fluorescence in Situ Hybridization on Cell Imprints

1997 ◽  
Vol 72 (3) ◽  
pp. 148-151 ◽  
Author(s):  
Nikiforos Kapranos ◽  
George Kontogeorgos ◽  
Konstantina Frangia ◽  
Eleni Kokka
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cytogenetic Analysis ◽  
Direct Fluorescence

Cytogenetic Analysis using Simultaneous and Sequential Fluorescence In Situ Hybridization

1998 ◽  
Vol 100 (2) ◽  
pp. 111-113 ◽  
Author(s):  
Tomás Escudero ◽  
Carmen Fuster ◽  
M.Dolors Coll ◽  
José Egozcue
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cytogenetic Analysis
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