Microplate methods for determination of serum cholesterol, high density lipoprotein cholesterol, triglyceride and apolipoproteins

Lipids ◽  
1993 ◽  
Vol 28 (2) ◽  
pp. 151-155 ◽  
Author(s):  
Rachel B. Shireman ◽  
Janine Durieux
Keyword(s):  
High Density Lipoprotein ◽  
Serum Cholesterol ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol

Comparison of the Du Pont aca and Dow methods for determination of high-density lipoprotein cholesterol.

1984 ◽  
Vol 30 (1) ◽  
pp. 127-129 ◽  
Author(s):  
N N Rehak ◽  
R J Elin ◽  
R Chesler ◽  
E Johnson
Keyword(s):  
High Density Lipoprotein ◽  
Disease Control ◽  
Total Cholesterol ◽  
High Density Lipoprotein Cholesterol ◽  
Dextran Sulfate ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Serum Samples

Abstract We compared the Du Pont aca (phosphotungstate-enzymic cholesterol) and the Dow (dextran sulfate/Mg2+-enzymic cholesterol) methods for the determination of high-density lipoprotein cholesterol (HDLC) and total cholesterol in serum from 113 patients. The aca results for both total cholesterol and HDLC were significantly greater (p less than 0.0001) than the Dow results, the aca method overestimating the HDLC concentration (mean recovery 107.2% in serum samples with values assigned by the Centers for Disease Control). The precision of the aca method for HDLC was essentially the same as that of the Dow method. Bilirubin (up to 0.17 g/L), hemoglobin (up to 4 g/L), and slight lipemia (triglycerides up to 5.4 g/L) did not interfere with the aca method.

Continuous-flow enzymic determination of serum total and high density lipoprotein cholesterol using microlitre sample volumes

1985 ◽  
Vol 18 (2) ◽  
pp. 134-137 ◽  
Author(s):  
Colin L.E. Jacklyn ◽  
Meng H. Tan ◽  
Vera A. Storm ◽  
Charman L. Cousins ◽  
Malcolm A. MacAulay
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Continuous Flow ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol

The effect of fasting status on the determination of low-density and high-density lipoprotein cholesterol

1995 ◽  
Vol 99 (4) ◽  
pp. 374-377 ◽  
Author(s):  
Lora B. Wilder ◽  
Paul S. Bachorik ◽  
Cheryl A. Finney ◽  
Taryn F. Moy ◽  
Diane M. Becker
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Low Density

Concentrations of lipids, lipoprotein, and apolipoproteins in serum of Zimbabwean Africans.

1985 ◽  
Vol 31 (8) ◽  
pp. 1390-1392 ◽  
Author(s):  
Z A Gomo
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Reference Ranges ◽  
European Populations

Abstract The subjects in this study were volunteers from a Zimbabwean population: 794 men and 705 women, ages between 20 and 65 years. They were receiving no medication and had no disease that could influence lipid metabolism. For determination of high-density lipoprotein cholesterol and apolipoproteins, they were screened for the known risk factors for coronary heart disease, to exclude factors known to influence those analytes. The results showed a significant sex- and age-dependence. The means and ranges for cholesterol, apolipoprotein B, and triglycerides were lower than those found in European populations. The high-density lipoprotein cholesterol and apolipoprotein A concentrations, on the other hand, were higher than in the European populations. This study established the reference ranges of the analytes studied and suggests that the prevalence of coronary heart disease may be low in Zimbabwean Africans.

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