Messenger RNA fluorescence in situ hybridization technique in tumor tissue sections

1995 ◽  
Vol 121 (S1) ◽  
pp. A31-A31
Author(s):  
V. Jung ◽  
S. Urbschat ◽  
S. Dooley ◽  
H. Bonkhoff ◽  
K. D. Zang
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Messenger Rna ◽  
Tumor Tissue ◽  
Hybridization Technique ◽  
Tissue Sections

Doing more with less: fluorescence in situ hybridization and gene sequencing assays can be reliably performed on archival stained tumor tissue sections

2016 ◽  
Vol 468 (4) ◽  
pp. 451-461 ◽  
Author(s):  
Giuseppe Pelosi ◽  
Federica Perrone ◽  
Elena Tamborini ◽  
Alessandra Fabbri ◽  
Maria Adele Testi ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Tumor Tissue ◽  
Gene Sequencing ◽  
Tissue Sections

Physical mapping of barley genes using an ultrasensitive fluorescence in situ hybridization technique

Genome ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 179-189 ◽  
Author(s):  
J L Stephens ◽  
S E Brown ◽  
N L.V Lapitan ◽  
D L Knudson
Keyword(s):  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Fluorescence In Situ Hybridization ◽  
Linkage Map ◽  
Physical Mapping ◽  
Physical Map ◽  
Primary Objective ◽  
Hybridization Technique ◽  
Hordeum Vulgare L

The primary objective of this study was to elucidate gene organization and to integrate the genetic linkage map for barley (Hordeum vulgare L.) with a physical map using ultrasensitive fluorescence in situ hybridization (FISH) techniques for detecting signals from restriction fragment length polymorphism (RFLP) clones. In the process, a single landmark plasmid, p18S5Shor, was constructed that identified and oriented all seven of the chromosome pairs. Plasmid p18S5Shor was used in all hybridizations. Fourteen cDNA probes selected from the linkage map for barley H. vulgare 'Steptoe' × H. vulgare 'Morex' (Kleinhofs et al. 1993) were mapped using an indirect tyramide signal amplification technique and assigned to a physical location on one or more chromosomes. The haploid barley genome is large and a complete physical map of the genome is not yet available; however, it was possible to integrate the linkage map and the physical locations of these cDNAs. An estimate of the ratio of base pairs to centimorgans was an average of 1.5 Mb/cM in the distal portions of the chromosome arms and 89 Mb/cM near the centromere. Furthermore, while it appears that the current linkage maps are well covered with markers along the length of each arm, the physical map showed that there are large areas of the genome that have yet to be mapped.Key words: Hordeum vulgare, barley, physical mapping, FISH, cDNA, genetics, linkage, chromosome, BACs.

scholarly journals The Use of Fluorescence In Situ Hybridization (FISH) on Paraffin-Embedded Tissue Sections for the Study of Microchimerism

BioTechniques ◽  
2000 ◽  
Vol 29 (6) ◽  
pp. 1220-1224 ◽  
Author(s):  
Kirby L. Johnson ◽  
Dong-Kai Zhen ◽  
Diana W. Bianchi
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Tissue Sections

scholarly journals Quick-FISH: A Rapid Fluorescence In Situ Hybridization Technique for Molecular Cytogenetic Analysis

BioTechniques ◽  
1998 ◽  
Vol 24 (5) ◽  
pp. 826-830 ◽  
Author(s):  
Sushanta K. Banerjee ◽  
Allan P. Weston ◽  
Diane L. Persons ◽  
Donald R. Campbell
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cytogenetic Analysis ◽  
Hybridization Technique ◽  
Molecular Cytogenetic

Fluorescence in situ hybridization for the identification of Treponema pallidum in tissue sections

2015 ◽  
Vol 305 (7) ◽  
pp. 709-718 ◽  
Author(s):  
Annett Petrich ◽  
Pablo Rojas ◽  
Julia Schulze ◽  
Christoph Loddenkemper ◽  
Lorenzo Giacani ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Treponema Pallidum ◽  
Tissue Sections

Detection of t(14;18) in follicular lymphoma by dual-color fluorescence in situ hybridization on paraffin-embedded tissue sections

2004 ◽  
Vol 150 (1) ◽  
pp. 22-26 ◽  
Author(s):  
Yosuke Matsumoto ◽  
Kenichi Nomura ◽  
Sawako Matsumoto ◽  
Kyoji Ueda ◽  
Mitsushige Nakao ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Follicular Lymphoma ◽  
Fluorescence In Situ Hybridization ◽  
Tissue Sections ◽  
Dual Color
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