scholarly journals The usefulness of two-colour fluorescence in situ hybridization technique using chromosomal subset at metaphase and interphase stage in radiation biodosimetry

2009 ◽  
Vol 2 (4) ◽  
pp. 1-11
Author(s):  
Kimio Tanaka
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Hybridization Technique ◽  
Radiation Biodosimetry

Physical mapping of barley genes using an ultrasensitive fluorescence in situ hybridization technique

Genome ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 179-189 ◽  
Author(s):  
J L Stephens ◽  
S E Brown ◽  
N L.V Lapitan ◽  
D L Knudson
Keyword(s):  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Fluorescence In Situ Hybridization ◽  
Linkage Map ◽  
Physical Mapping ◽  
Physical Map ◽  
Primary Objective ◽  
Hybridization Technique ◽  
Hordeum Vulgare L

The primary objective of this study was to elucidate gene organization and to integrate the genetic linkage map for barley (Hordeum vulgare L.) with a physical map using ultrasensitive fluorescence in situ hybridization (FISH) techniques for detecting signals from restriction fragment length polymorphism (RFLP) clones. In the process, a single landmark plasmid, p18S5Shor, was constructed that identified and oriented all seven of the chromosome pairs. Plasmid p18S5Shor was used in all hybridizations. Fourteen cDNA probes selected from the linkage map for barley H. vulgare 'Steptoe' × H. vulgare 'Morex' (Kleinhofs et al. 1993) were mapped using an indirect tyramide signal amplification technique and assigned to a physical location on one or more chromosomes. The haploid barley genome is large and a complete physical map of the genome is not yet available; however, it was possible to integrate the linkage map and the physical locations of these cDNAs. An estimate of the ratio of base pairs to centimorgans was an average of 1.5 Mb/cM in the distal portions of the chromosome arms and 89 Mb/cM near the centromere. Furthermore, while it appears that the current linkage maps are well covered with markers along the length of each arm, the physical map showed that there are large areas of the genome that have yet to be mapped.Key words: Hordeum vulgare, barley, physical mapping, FISH, cDNA, genetics, linkage, chromosome, BACs.

scholarly journals Quick-FISH: A Rapid Fluorescence In Situ Hybridization Technique for Molecular Cytogenetic Analysis

BioTechniques ◽  
1998 ◽  
Vol 24 (5) ◽  
pp. 826-830 ◽  
Author(s):  
Sushanta K. Banerjee ◽  
Allan P. Weston ◽  
Diane L. Persons ◽  
Donald R. Campbell
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cytogenetic Analysis ◽  
Hybridization Technique ◽  
Molecular Cytogenetic

Messenger RNA fluorescence in situ hybridization technique in tumor tissue sections

1995 ◽  
Vol 121 (S1) ◽  
pp. A31-A31
Author(s):  
V. Jung ◽  
S. Urbschat ◽  
S. Dooley ◽  
H. Bonkhoff ◽  
K. D. Zang
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Messenger Rna ◽  
Tumor Tissue ◽  
Hybridization Technique ◽  
Tissue Sections
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