A histological study of the short-term effects of fluoride on enamel and dentine formation in hamster tooth-germs in organ culture in vitro

1984 ◽  
Vol 29 (10) ◽  
pp. 803-810 ◽  
Author(s):  
A.L.J.J. Bronckers ◽  
Lyda L. Jansen ◽  
J.H.M. Wöltgens
Keyword(s):  
Organ Culture ◽  
Histological Study ◽  
Short Term ◽  
Culture In Vitro ◽  
Tooth Germs ◽  
Short Term Effects

IN VITRO METHODS FOR THE STUDY OF THE ADENOHYPOPHYSIAL FUNCTIONS TO SECRETE GONADOTROPHIN

1971 ◽  
Vol 68 (1_Suppl) ◽  
pp. S27-S40 ◽  
Author(s):  
T. Kobayashi ◽  
T. Kigawa ◽  
M. Mizuno ◽  
T. Watanabe
Keyword(s):  
Cell Culture ◽  
Organ Culture ◽  
Cultured Cells ◽  
Cell Sheet ◽  
Short Term ◽  
Hypothalamic Extract ◽  
Numerous Cell ◽  
Single Cell Culture ◽  
Almost All

ABSTRACT There are several in vitro methods to analyse the function of the adenohypophysis or the mechanisms of its regulation. The present paper deals with single cell culture, organ culture and short term incubation techniques by which the morphology and gonadotrophin-secreting function of the adenohypophysis were studied. In trypsin-dispersed cell culture, the adenohypophysial cells showed extensive propagation to form numerous cell colonies and finally develop into a confluent monolayer cell sheet covering completely the surface of culture vessels. Almost all of the cultured cells, however, became chromophobic, at least at the end of the first week of cultivation, when gonadotrophin was detectable neither in the culture medium nor in the cells themselves. After the addition of the hypothalamic extract, gonadotrophin became detectable again, and basophilic or PAS-positive granules also reappeared within the cells, suggesting that the gonadotrophs were stimulated by the extract to produce gonadotrophin. In organ culture and short term incubation, the incorporation of [3H] leucine into the adenohypophysial cells in relation to the addition of hypothalamic extract was examined. It was obvious that the ability to incorporate [3H] leucine into the gonadotrophs in vitro was highly dependent upon the presence of the hypothalamic extract.

Effect of CO2 on short-term human lymphocyte culture in vitro

In Vitro ◽  
1977 ◽  
Vol 13 (11) ◽  
pp. 806-807 ◽  
Author(s):  
Ram S. Verma ◽  
Carole Rubenstein ◽  
Harvey Dosik
Keyword(s):  
Human Lymphocyte ◽  
Lymphocyte Culture ◽  
Short Term ◽  
Culture In Vitro

scholarly journals Short-Term Cigarette Smoking in Rats Impairs Physical Capacity and Induces Cardiac Remodeling

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Danilo Sales Bocalini ◽  
Rafael da Silva Luiz ◽  
Kleiton Augusto Santos Silva ◽  
Andrey Jorge Serra ◽  
Renata Andrade Avila ◽  
...  
Keyword(s):  
Renal Function ◽  
Cigarette Smoking ◽  
Cardiac Remodeling ◽  
Left Ventricular ◽  
Physical Capacity ◽  
Myocardial Performance ◽  
Short Term ◽  
Smoking Group ◽  
Short Term Effects

Despite the strong evidence on the cardiac and renal damages after chronic exposure to cigarette smoke, there is a paucity of data on its short-term effects. The study evaluated the short-term effects of cigarette smoking on left ventricular (LV) remodeling, in vitro myocardial and renal function. Female Wistar rats were randomized to control (C) and cigarette smoking rats for eight weeks. Physical capacity was assessed using an adapted model of exhaustive swim; left ventricle (LV) morphology and function were also evaluated. Renal function was assessed by creatinine clearance and urine protein. The in vitro myocardial performance was analyzed in isolated papillary muscles. Rats exhibited reduced physical capacity after short-term cigarette smoking. Although there was no change on LV function, reduced chamber diameter was found in the smoking group associated with an increased LV wall thickness. There was augmented cardiac mass compared to C that was confirmed by increased cardiomyocyte nucleus volume, but in vitro myocardial performance and renal function were unchanged. A short-term cigarette smoking induces cardiac remodeling without abnormalities in function. The smoking group still preserved renal function and in vitro myocardial performance. However, the reduced physical capacity may suggest an impairment of the cardiac reserve.

CIRCADIAN VARIATIONS IN ADRENAL SECRETION OF LEMMINGS, VOLES AND MICE

1970 ◽  
Vol 65 (4) ◽  
pp. 645-649 ◽  
Author(s):  
Richard V. Andrews
Keyword(s):  
Locomotor Activity ◽  
Organ Culture ◽  
Circadian Variation ◽  
The Arctic ◽  
Summer Solstice ◽  
Circadian Variations ◽  
Short Term ◽  
Culture Methods ◽  
Adrenal Secretion

ABSTRACT Daily variations in adrenal secretion by glands from arctic rodents were measured in vitro. Serial-sacrifice, short-term incubation studies yield similar results to those data obtained through organ-culture methods. Adrenal secretory rates display some desynchronization from locomotor activity during the summer solstice, although circadian variation persists during all seasons of the arctic year.

An in vitro/in vivo study into the short term effects of exposure to mineral fibres

1996 ◽  
Vol 48 (6) ◽  
pp. 484-486 ◽  
Author(s):  
A. Clouter ◽  
C.E. Houghton ◽  
C.A. Bowskill ◽  
J.A. Hoskins ◽  
R.C. Brown
Keyword(s):  
In Vivo Study ◽  
Short Term ◽  
Short Term Effects
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