Fish cell culture: Establishment of two fibroblast-like cell lines (OL-17 and OL-32) from fins of the medaka,Oryzias latipes

1988 ◽  
Vol 24 (4) ◽  
pp. 294-298 ◽  
Author(s):  
Jun-Ichiro Komura ◽  
Hiroshi Mitani ◽  
Akihiro Shima
Keyword(s):  
Cell Culture ◽  
Cell Lines ◽  
Oryzias Latipes ◽  
Fish Cell ◽  
Fish Cell Culture

Three decades of fish cell culture: A current listing of cell lines derived from fishes

1994 ◽  
Vol 16 (2) ◽  
pp. 87-94 ◽  
Author(s):  
J. L. Fryer ◽  
C. N. Lannan
Keyword(s):  
Cell Culture ◽  
Cell Lines ◽  
Fish Cell ◽  
Fish Cell Culture ◽  
A Current

Status, challenges, and perspectives of fish cell culture—Focus on cell lines capable of in vitro mineralization

2022 ◽  
pp. 381-404
Author(s):  
Vincent Laizé ◽  
Joana T. Rosa ◽  
Marco Tarasco ◽  
M. Leonor Cancela
Keyword(s):  
Cell Culture ◽  
Cell Lines ◽  
Fish Cell ◽  
Fish Cell Culture ◽  
In Vitro Mineralization

Fish cell culture: Characteristics of a cell line from the silver perch,Bairdiella chrysura

In Vitro ◽  
1977 ◽  
Vol 13 (6) ◽  
pp. 389-397 ◽  
Author(s):  
J. H. Wharton ◽  
R. D. Ellender ◽  
B. L. Middlebrooks ◽  
P. K. Stocks ◽  
Adrian R. Lawler ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Fish Cell ◽  
Fish Cell Culture ◽  
Culture Characteristics ◽  
A Cell

Fish cell culture: A new cell line fromCynoscion nebulosus

In Vitro ◽  
1979 ◽  
Vol 15 (2) ◽  
pp. 109-111 ◽  
Author(s):  
B. L. Middlebrooks ◽  
R. D. Ellender ◽  
J. H. Wharton
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Fish Cell ◽  
New Cell Line ◽  
Fish Cell Culture

Fish cell culture: A fibroblastic line (PG) from ovaries of juvenile pike (Esox lucius)

In Vitro ◽  
1979 ◽  
Vol 15 (11) ◽  
pp. 839-840 ◽  
Author(s):  
W. Ahne
Keyword(s):  
Cell Culture ◽  
Esox Lucius ◽  
Fish Cell ◽  
Pike Esox Lucius ◽  
Fish Cell Culture

Fish Cell Culture: Properties of a Cell Line from the Sheepshead, Archosargus probatocephalus

1978 ◽  
Vol 35 (4) ◽  
pp. 470-473 ◽  
Author(s):  
W. M. Law ◽  
R. D. Ellender ◽  
J. H. Wharton ◽  
B. L. Middlebrooks
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Chloride Concentration ◽  
Antibody Test ◽  
Lactic Dehydrogenase ◽  
Fish Cell ◽  
Cytotoxic Antibody ◽  
Fish Cell Culture ◽  
A Cell ◽  
Key Words Fish

A cell line designated SHF-1 was established from fin tissue of the sheepshead, Archosargus probatocephalus. Cells were fibroblastlike and grew best at 26 °C in Leibovitz (L-15) medium containing serum supplements and a final sodium chloride concentration of 0.150 M. The 2N number of primary sheepshead cells was 48 but the SHF-1 line at passage levels above 11 was heteroploid. SHF-1 cells did not replicate any of seven viruses tested. Species confirmation of SHF-1 was accomplished by the cytotoxic antibody test and was attempted with isoelectric focusing of lactic dehydrogenase isoenzymes. Plating efficiency and freeze viability studies were also performed. Key words: fish cell culture, sheepshead, Archosargus probatocephalus, microbiology

Fish Cell Culture: An Update

1989 ◽  
pp. 1-18 ◽  
Author(s):  
Bruce L. Nicholson
Keyword(s):  
Cell Culture ◽  
Fish Cell ◽  
Fish Cell Culture

scholarly journals Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio)

2020 ◽  
Vol 12 (1) ◽  
pp. 73
Author(s):  
Insariani Insariani ◽  
Trisniaty Trisniaty ◽  
Freddy Riatmono ◽  
Abdul Ghani
Keyword(s):  
Cell Culture ◽  
Cell Lines ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Primary Cell ◽  
Fish Cell ◽  
Caudal Fin ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Primary Cell Lines

HighlihgtDevelop primary cultures derived From  tissue tails fins, gills, kidney and spleen from local Indonesian carp (Cyprinus carpio).Primary culture cell with L15  Mediacell cultures  consist of two type Fibroblast-like and epithelial –like cell AbstractThe fish cell lines technology have been developed for the interests of the fisheries world. This study aimed at developing a primary cell line from gill, kidney, spleen, and caudal fin of a common carp (Cyprinus carpio). A healthy common carp weighing 20 g (~1 month) was collected from the Cijeruk Fish Seed Center, Bogor. The development of primary cell lines from the gill, fin, tail, kidney and spleen tissue was performed in cell culture medium Leibovitz’s L-15 supplemented with 20% serum fetal bovine, 250 IU Penicillin, 250 µg / ml kanamycin sulfate and 2Mm L-Glutamine, and incubated at 28°C. Primary cell lines of caudal fin and gill began to form a monolayer on day 17 after culture. While the development of cell lines from kidney and spleen, although the initiation of cells and cells spread on the surface into a monolayer, was not perfect; therefore, the passage was unable to be done. Microscopic observations and Giemsa staining showed primary cell lines of caudal fin and gill based on cell morphology consisted of two cell types, fibroblast-like cells and epithelial-like cells. The first passage was done on day 17 when the confluence was more than 50%. The next passage was carried out every 3 weeks when confluence reached 70% -80%. The primary cell culture of gill was successfully passaged as much as 72 and the caudal fin was successfully passed as much as 89 times over 7 years. These new cell lines can be further used to propagate fish viruses and other biotechnology assays.

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