Fish cell culture: A new cell line fromCynoscion nebulosus

In Vitro ◽  
1979 ◽  
Vol 15 (2) ◽  
pp. 109-111 ◽  
Author(s):  
B. L. Middlebrooks ◽  
R. D. Ellender ◽  
J. H. Wharton
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Fish Cell ◽  
New Cell Line ◽  
Fish Cell Culture

Fish cell culture: Characteristics of a cell line from the silver perch,Bairdiella chrysura

In Vitro ◽  
1977 ◽  
Vol 13 (6) ◽  
pp. 389-397 ◽  
Author(s):  
J. H. Wharton ◽  
R. D. Ellender ◽  
B. L. Middlebrooks ◽  
P. K. Stocks ◽  
Adrian R. Lawler ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Fish Cell ◽  
Fish Cell Culture ◽  
Culture Characteristics ◽  
A Cell

Fish Cell Culture: Properties of a Cell Line from the Sheepshead, Archosargus probatocephalus

1978 ◽  
Vol 35 (4) ◽  
pp. 470-473 ◽  
Author(s):  
W. M. Law ◽  
R. D. Ellender ◽  
J. H. Wharton ◽  
B. L. Middlebrooks
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Chloride Concentration ◽  
Antibody Test ◽  
Lactic Dehydrogenase ◽  
Fish Cell ◽  
Cytotoxic Antibody ◽  
Fish Cell Culture ◽  
A Cell ◽  
Key Words Fish

A cell line designated SHF-1 was established from fin tissue of the sheepshead, Archosargus probatocephalus. Cells were fibroblastlike and grew best at 26 °C in Leibovitz (L-15) medium containing serum supplements and a final sodium chloride concentration of 0.150 M. The 2N number of primary sheepshead cells was 48 but the SHF-1 line at passage levels above 11 was heteroploid. SHF-1 cells did not replicate any of seven viruses tested. Species confirmation of SHF-1 was accomplished by the cytotoxic antibody test and was attempted with isoelectric focusing of lactic dehydrogenase isoenzymes. Plating efficiency and freeze viability studies were also performed. Key words: fish cell culture, sheepshead, Archosargus probatocephalus, microbiology

Growth Rates of a New Cell Line from Channel Catfish Ovary and Channel Catfish Virus Replication at Different Temperatures

1980 ◽  
Vol 37 (5) ◽  
pp. 871-873 ◽  
Author(s):  
P. R. Bowser ◽  
J. A. Plumb
Keyword(s):  
Cell Line ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Growth Rates ◽  
Frozen Storage ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Fish Cell ◽  
Channel Catfish Virus ◽  
New Cell Line

Growth rates of a new cell line from the channel catfish, Ictalurus punctatus, were determined at temperatures from 10 to 35 °C. Growth was more rapid at 35 °C with the cells having a population doubling time of 12 h, and cell numbers declined at 10 °C. The cells survived frozen storage at −70 °C for up to 6 mo. Replication of channel catfish virus occurred from 10 to 35 °C. Although it was most rapid at 35 °C, maximum replication occurred at 30 °C.Key words: fish viruses, channel catfish virus, herpesvirus, fish cell cultures, fish diseases, cell growth rates

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