Fish cell culture: Initiation of a line of pituitary cells from carp (Cyprinus carpio) to study the release of gonadotropin in vitro

In Vitro ◽  
1982 ◽  
Vol 18 (5) ◽  
pp. 419-420 ◽  
Author(s):  
L. Ribeiro ◽  
W. Ahne
Keyword(s):  
Cell Culture ◽  
Cyprinus Carpio ◽  
Fish Cell ◽  
Pituitary Cells ◽  
Culture Initiation ◽  
Fish Cell Culture ◽  
Carp Cyprinus Carpio

Status, challenges, and perspectives of fish cell culture—Focus on cell lines capable of in vitro mineralization

2022 ◽  
pp. 381-404
Author(s):  
Vincent Laizé ◽  
Joana T. Rosa ◽  
Marco Tarasco ◽  
M. Leonor Cancela
Keyword(s):  
Cell Culture ◽  
Cell Lines ◽  
Fish Cell ◽  
Fish Cell Culture ◽  
In Vitro Mineralization

scholarly journals Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio)

2020 ◽  
Vol 12 (1) ◽  
pp. 73
Author(s):  
Insariani Insariani ◽  
Trisniaty Trisniaty ◽  
Freddy Riatmono ◽  
Abdul Ghani
Keyword(s):  
Cell Culture ◽  
Cell Lines ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Primary Cell ◽  
Fish Cell ◽  
Caudal Fin ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Primary Cell Lines

HighlihgtDevelop primary cultures derived From  tissue tails fins, gills, kidney and spleen from local Indonesian carp (Cyprinus carpio).Primary culture cell with L15  Mediacell cultures  consist of two type Fibroblast-like and epithelial –like cell AbstractThe fish cell lines technology have been developed for the interests of the fisheries world. This study aimed at developing a primary cell line from gill, kidney, spleen, and caudal fin of a common carp (Cyprinus carpio). A healthy common carp weighing 20 g (~1 month) was collected from the Cijeruk Fish Seed Center, Bogor. The development of primary cell lines from the gill, fin, tail, kidney and spleen tissue was performed in cell culture medium Leibovitz’s L-15 supplemented with 20% serum fetal bovine, 250 IU Penicillin, 250 µg / ml kanamycin sulfate and 2Mm L-Glutamine, and incubated at 28°C. Primary cell lines of caudal fin and gill began to form a monolayer on day 17 after culture. While the development of cell lines from kidney and spleen, although the initiation of cells and cells spread on the surface into a monolayer, was not perfect; therefore, the passage was unable to be done. Microscopic observations and Giemsa staining showed primary cell lines of caudal fin and gill based on cell morphology consisted of two cell types, fibroblast-like cells and epithelial-like cells. The first passage was done on day 17 when the confluence was more than 50%. The next passage was carried out every 3 weeks when confluence reached 70% -80%. The primary cell culture of gill was successfully passaged as much as 72 and the caudal fin was successfully passed as much as 89 times over 7 years. These new cell lines can be further used to propagate fish viruses and other biotechnology assays.

scholarly journals  Steroid effects on the in vitro LH secretion from pituitary cells of sexually immature carp (Cyprinus carpio L.) under the influence of naltrexone and morphine

2010 ◽  
Vol 55 (No. 6) ◽  
pp. 250-257
Author(s):  
M. Sokolowska-Mikolajczyk ◽  
M. Socha ◽  
P. Epler
Keyword(s):  
Cyprinus Carpio ◽  
Sex Steroids ◽  
Pituitary Cells ◽  
Control Medium ◽  
Opioid Agonist ◽  
Carp Cyprinus Carpio ◽  
Lh Release ◽  
Lh Secretion

Dispersed cells from sexually immature carp (footlings) pituitaries were exposed to estradiol (E<sub>2</sub>) or testosterone (T) (both at 3 &times; 10<sup>&ndash;8</sup> M) in the presence of opioid receptor antagonist naltrexone (10<sup>&ndash;8</sup> or 10<sup>&ndash;6</sup> M) and/or agonist &ndash; morphine (10<sup>&ndash;8</sup> or 10<sup>&ndash;6</sup> M). Naltrexone alone at 10<sup>&ndash;6</sup> M increased the LH level as compared with the control. Morphine (10<sup>&ndash;8</sup> or 10<sup>&ndash;6</sup> M), T and E2 had no influence on LH levels. The combination of T with naltrexone (10<sup>&ndash;8</sup> M) stimulated LH release if compared with the control or with T alone. Morphine (both concentrations) with T caused significantly higher LH secretion than the control medium and T alone. Estradiol with naltrexone (10<sup>&ndash;8</sup> and 10<sup>&ndash;6</sup> M) had no influence on LH concentration. In media with E2 and morphine (10<sup>&ndash;8</sup> M) LH levels were higher than in the control and estradiol alone. The results show that in common carp sex steroids affect the response of pituitary cells to opioid agonist or antagonist giving an evidence on the role of steroids in LH release mediated by the opioid system.

Evaluating in vitro dipeptidyl peptidase IV inhibition by peptides from common carp (Cyprinus carpio) roe in cell culture models

2019 ◽  
Vol 246 (1) ◽  
pp. 179-191 ◽  
Author(s):  
Changyu Zhang ◽  
Song Gao ◽  
Chi Zhang ◽  
Yuqi Zhang ◽  
Huaigao Liu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Dipeptidyl Peptidase Iv ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Culture Models ◽  
Dipeptidyl Peptidase Iv Inhibition ◽  
Cell Culture Models

Fish cell culture: Characteristics of a cell line from the silver perch,Bairdiella chrysura

In Vitro ◽  
1977 ◽  
Vol 13 (6) ◽  
pp. 389-397 ◽  
Author(s):  
J. H. Wharton ◽  
R. D. Ellender ◽  
B. L. Middlebrooks ◽  
P. K. Stocks ◽  
Adrian R. Lawler ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Fish Cell ◽  
Fish Cell Culture ◽  
Culture Characteristics ◽  
A Cell

scholarly journals In vitro cultivation of Trypanoplasma borreli (Protozoa:Kinetoplastida), a parasite from the blood of common carp Cyprinus carpio

2000 ◽  
Vol 41 ◽  
pp. 195-201 ◽  
Author(s):  
D Steinhagen ◽  
W Hedderich ◽  
A Skouras ◽  
JP Scharsack ◽  
J Schuberth ◽  
...  
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
In Vitro Cultivation ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

scholarly journals The in vitro toxicity of atrazine on kinematics and DNA fragmentation in common carp (Cyprinus carpio) sperm cells

2019 ◽  
Vol 70 (3) ◽  
pp. 1639
Author(s):  
M.E. ÖZGÜR
Keyword(s):  
Dna Fragmentation ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
In Vitro Toxicity ◽  
Computer Assisted ◽  
Sperm Cells ◽  
Common Carp Cyprinus Carpio ◽  
Significant Difference ◽  
Carp Cyprinus Carpio

This study investigated the in vitro effects of different concentrations of Atrazine (0.001, 0.01, 0.1, 0.5 mg/L) added to motile and immotile solutions on kinematics quality of sperm cells of common carp, Cyprinus carpio, which is a fish of economic significance. The kinematics of the sperm cells was analyzed by a computer-assisted sperm analysis system (CASA). As a result of the study, while there was a significant difference (P < 0.05) between the groups in terms of the VSL (μm/s) and VCL (μm/s) values after the Atrazine-added immotile solution’s (IMS) and incubation for 3 hours, there was a significant difference (P < 0.05) in only the VSL values directly activated by the Atrazine-added motile solution (MS). DNA fragmentation was evident but not in higher numbers in the 0.1 mg/L atrazine group. Finally, it was determined the effective concentration (EC50) values of the VSL value of the motile and immotile solution as 0.34 mg/L and 0.03 mg/L, respectively.

Effects of Bothriocephalus acheilognathi on the polarization response of pronephric leucocytes of carp, Cyprinus carpio

2000 ◽  
Vol 74 (3) ◽  
pp. 253-257 ◽  
Author(s):  
P. Nie ◽  
D. Hoole
Keyword(s):  
Cyprinus Carpio ◽  
Infected Fish ◽  
Polarization Response ◽  
Leucocyte Migration ◽  
Significant Difference ◽  
Carp Cyprinus Carpio ◽  
Bothriocephalus Acheilognathi ◽  
Carp Serum

AbstractAn in vitro assay was used to examine the effect of Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda: Pseudophyllidea) on the polarization response of pronephric leucocytes of carp, Cyprinus carpio. Leucocytes, isolated from naive, naturally-infected fish and carp injected intraperitoneally with cestode extracts, were exposed to parasite extracts (protein concentrations 0–10.0 μg ml-1), for up to 24 h in the presence or absence of carp serum. In general, polarization responses of the pronephric leucocytes, primarily neutrophils and eosinophils, increased with incubation time although there was no significant difference in the response induced by the different protein concentrations. Differences in the polarization response were, however, observed in naive, naturally infected and injected fish and the cells responded differently in the presence and absence of carp serum. In the absence of carp serum the polarization response of pronephric leucocytes in vitro was significantly reduced with cells obtained from injected and naturally infected fish compared with those obtained from naive carp. This suppression of leucocyte migration was however reduced by the addition of carp serum to the in vitro system. The role of this interaction between the possible suppression of polarization induced by the parasite and stimulation by serum is discussed.

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