Evidence for secretion of high molecular weight mucins by canine tracheal epithelial cells in primary culture: Effects of select secretagogues on mucin secretion

1992 ◽  
Vol 28 (2) ◽  
pp. 120-127 ◽  
Author(s):  
Arvind K. Virmani ◽  
Bashoo Naziruddin ◽  
Vinay C. Desai ◽  
Jon P. Lowry ◽  
Donald C. Graves ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Epithelial Cells ◽  
Primary Culture ◽  
High Molecular Weight ◽  
Mucin Secretion ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial ◽  
Culture Effects

Residual Oil Fly Ash Induces Cytotoxicity and Mucin Secretion by Guinea Pig Tracheal Epithelial Cells via an Oxidant-Mediated Mechanism

2000 ◽  
Vol 163 (3) ◽  
pp. 221-230 ◽  
Author(s):  
Nanfei Jiang ◽  
Kevin L. Dreher ◽  
Janice A. Dye ◽  
Yuehua Li ◽  
Judy H. Richards ◽  
...  
Keyword(s):  
Fly Ash ◽  
Epithelial Cells ◽  
Guinea Pig ◽  
Residual Oil ◽  
Mucin Secretion ◽  
Tracheal Epithelial Cells ◽  
Residual Oil Fly Ash ◽  
Tracheal Epithelial ◽  
Oil Fly Ash

Effects of retinoic acid on the growth and morphology of hamster tracheal epithelial cells in primary culture

1987 ◽  
Vol 54 (1) ◽  
pp. 38-51 ◽  
Author(s):  
Elizabeth M. McDowell ◽  
Theresa Ben ◽  
Bill Coleman ◽  
Seung Chang ◽  
Carnell Newkirk ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Epithelial Cells ◽  
Primary Culture ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial

Growth Requirements of Ferret Tracheal Epithelial Cells in Primary Culture

1985 ◽  
Vol 179 (3) ◽  
pp. 309-317 ◽  
Author(s):  
J. W. Groelke ◽  
J. J. Coalson ◽  
J. B. Baseman
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Tracheal Epithelial Cells ◽  
Growth Requirements ◽  
Tracheal Epithelial

scholarly journals Primary Culture of Chicken Tracheal Epithelial Cells and Study on Those Characters for Recombinant Virus Infection

2013 ◽  
Vol 40 (4) ◽  
pp. 299-304
Author(s):  
Mi Na Park ◽  
Hyun-Jun Jang ◽  
Dae Ho Keum ◽  
Jin Ae Choi ◽  
Jae Gyu Yoo ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Virus Infection ◽  
Primary Culture ◽  
Recombinant Virus ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial

Extracellular matrix-dependent differentiation of rabbit tracheal epithelial cells in primary culture

1994 ◽  
Vol 30 (1) ◽  
pp. 56-67 ◽  
Author(s):  
Armelle Baeza-Squiban ◽  
Emmanuelle Boisvieux-Ulrich ◽  
Catherine Guilianelli ◽  
Odile Houcine ◽  
Gérard Geraud ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cells ◽  
Primary Culture ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial

Beta-adrenergic receptors on human tracheal epithelial cells in primary culture

1990 ◽  
Vol 258 (1) ◽  
pp. C71-C76 ◽  
Author(s):  
P. B. Davis ◽  
C. L. Silski ◽  
C. M. Kercsmar ◽  
M. Infeld
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Adrenergic Receptors ◽  
Platelet Activating Factor ◽  
Receptor Density ◽  
Beta Adrenergic ◽  
Adrenergic Agents ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial ◽  
Beta 2

Human tracheal epithelial cells in suspension, whether obtained by brushing at bronchoscopy or from necropsy specimens by proteolytic digestion and EDTA treatment, increase adenosine 3',5'-cyclic monophosphate (cAMP) production in response to isoproterenol. These cells in primary culture also respond to beta-adrenergic agonists in order of potency isoproterenol greater than epinephrine greater than norepinephrine. The response is inhibited by propranolol or ICI 118551 (a beta 2-adrenergic selective blocker) but not by atenolol (a beta 1-adrenergic blocker). Binding of [125I]iodocyanopindolol (ICYP) to membranes was rapid, stereoselective, and saturable and displayed receptor density 8.0 +/- 4.6 fmol/mg protein (mean 228 receptors/cell) and a dissociation constant (KD) for ICYP of 35 +/- 14 pM for freshly isolated cells and a KD of 25 +/- 13 pM and receptor density of 17 +/- 17 fmol/mg protein for cells in culture. The 50% inhibitory concentration (IC50) for atenolol was 470 microM and for ICI 118551 was 0.012 microM. Analysis of the ICI 118551 displacement curve indicates that greater than 90% of the receptors are of the beta 2-adrenergic class. Prostaglandins E1 or E2, vasoactive intestinal peptide, carbachol, phenylephrine, or platelet-activating factor did not affect either the maximal cAMP response or the isoproterenol dose-response relationship. Neither clonidine nor epinephrine plus propranolol altered cellular cAMP content, and cyclooxygenase inhibition did not change the cAMP response to epinephrine. We conclude that in human tracheal epithelial cells in primary culture, adrenergic stimulation affects cAMP levels only through beta 2-adrenergic receptors and that modulation of this system by platelet-activating factor or muscarinic, alpha 1-, or alpha 2-adrenergic agents does not occur.

Sign in / Sign up

Export Citation Format

Share Document