Effects of retinoic acid on the growth and morphology of hamster tracheal epithelial cells in primary culture

1987 ◽  
Vol 54 (1) ◽  
pp. 38-51 ◽  
Author(s):  
Elizabeth M. McDowell ◽  
Theresa Ben ◽  
Bill Coleman ◽  
Seung Chang ◽  
Carnell Newkirk ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Epithelial Cells ◽  
Primary Culture ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial

scholarly journals Molecular cloning of gene sequences regulated during squamous differentiation of tracheal epithelial cells and controlled by retinoic acid.

1987 ◽  
Vol 7 (11) ◽  
pp. 4017-4023 ◽  
Author(s):  
H L Smits ◽  
E E Floyd ◽  
A M Jetten
Keyword(s):  
Retinoic Acid ◽  
Epithelial Cells ◽  
Cdna Library ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Squamous Differentiation ◽  
Squamous Cells ◽  
Tracheal Epithelial Cells ◽  
Molecular Events ◽  
Tracheal Epithelial

A cDNA library was constructed from polyadenylated RNA present in squamous differentiated rabbit tracheal epithelial cells. Screening of the cDNA library was aimed at identifying RNAs that were abundant in squamous cells and expressed at low levels in undifferentiated cells. Two different recombinants were obtained containing inserts, 0.86 and 0.77 kilobases (kb) in size, that hybridized to mRNAs 1.0 and 1.25 kb in length. These RNAs were present at approximately 50-fold higher levels in squamous cells than in proliferative or confluent retinoic acid-treated cells. The increase in the levels of the 1.0- and 1.25-kb RNAs correlated closely with the onset of squamous differentiation and was not related to induction of terminal cell division. Treatment of rabbit tracheal epithelial cells with transforming growth factor beta, which induces squamous differentiation in these cells, also resulted in elevated levels of the 1.0- and 1.25-kb RNAs. The increased levels of these RNAs in squamous cells appeared to a large extent to be regulated at a posttranscriptional level. Retinoic acid not only inhibited the increase in the levels of the 1.0- and 1.25-kb RNAs but also reversed the expression of these RNAs in squamous cells. These results suggest that retinoic acid affects, directly or indirectly, molecular events that induce alterations in the posttranscriptional processing of the transcripts corresponding to the 1.0- and 1.25-kb RNAs.

Regulation of type I (epidermal) transglutaminase mRNA levels during squamous differentiation: down regulation by retinoids

1989 ◽  
Vol 9 (11) ◽  
pp. 4846-4851
Author(s):  
E E Floyd ◽  
A M Jetten
Keyword(s):  
Retinoic Acid ◽  
Epithelial Cells ◽  
Cdna Clones ◽  
Type I ◽  
Mrna Levels ◽  
Type Ii ◽  
Squamous Differentiation ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial ◽  
Epidermal Transglutaminase

Squamous differentiation of rabbit tracheal epithelial cells is accompanied by an approximately 50-fold increase in the activity of type I (epidermal) transglutaminase, while the levels of type II (tissue) transglutaminase remain almost undetectable. To identify a cDNA encoding type I transglutaminase, we screened a library of cDNA clones prepared from poly(A)+ RNA isolated from squamous-differentiated rabbit tracheal epithelial cells. Four overlapping clones (represented by clone pTG-7) which span a range of 2.8 kilobases were identified; partial sequencing of pTG-7 indicated that it encodes a transglutaminaselike protein. pTG-7 hybridized to a 3.6-kilobase mRNA which is distinct from that for type II transglutaminase. pTG-7 mRNA levels were low in proliferative cells, increased dramatically in squamous-differentiated cells, and could be further enhanced by growth of the cells in high concentrations (2 mM) of calcium ions. Retinoic acid, which blocks the expression of the squamous phenotype, prevented this increase in pTG-7 mRNA levels. These changes in levels of pTG-7 mRNA parallel the changes in type I transglutaminase activity observed under similar culture conditions. These data indicate that pTG-7 encodes the mRNA for transglutaminase type I and that expression of this mRNA is negatively regulated by retinoic acid.

Molecular cloning of gene sequences regulated during squamous differentiation of tracheal epithelial cells and controlled by retinoic acid

1987 ◽  
Vol 7 (11) ◽  
pp. 4017-4023
Author(s):  
H L Smits ◽  
E E Floyd ◽  
A M Jetten
Keyword(s):  
Retinoic Acid ◽  
Epithelial Cells ◽  
Cdna Library ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Squamous Differentiation ◽  
Squamous Cells ◽  
Tracheal Epithelial Cells ◽  
Molecular Events ◽  
Tracheal Epithelial

A cDNA library was constructed from polyadenylated RNA present in squamous differentiated rabbit tracheal epithelial cells. Screening of the cDNA library was aimed at identifying RNAs that were abundant in squamous cells and expressed at low levels in undifferentiated cells. Two different recombinants were obtained containing inserts, 0.86 and 0.77 kilobases (kb) in size, that hybridized to mRNAs 1.0 and 1.25 kb in length. These RNAs were present at approximately 50-fold higher levels in squamous cells than in proliferative or confluent retinoic acid-treated cells. The increase in the levels of the 1.0- and 1.25-kb RNAs correlated closely with the onset of squamous differentiation and was not related to induction of terminal cell division. Treatment of rabbit tracheal epithelial cells with transforming growth factor beta, which induces squamous differentiation in these cells, also resulted in elevated levels of the 1.0- and 1.25-kb RNAs. The increased levels of these RNAs in squamous cells appeared to a large extent to be regulated at a posttranscriptional level. Retinoic acid not only inhibited the increase in the levels of the 1.0- and 1.25-kb RNAs but also reversed the expression of these RNAs in squamous cells. These results suggest that retinoic acid affects, directly or indirectly, molecular events that induce alterations in the posttranscriptional processing of the transcripts corresponding to the 1.0- and 1.25-kb RNAs.

Growth Requirements of Ferret Tracheal Epithelial Cells in Primary Culture

1985 ◽  
Vol 179 (3) ◽  
pp. 309-317 ◽  
Author(s):  
J. W. Groelke ◽  
J. J. Coalson ◽  
J. B. Baseman
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Tracheal Epithelial Cells ◽  
Growth Requirements ◽  
Tracheal Epithelial

scholarly journals Primary Culture of Chicken Tracheal Epithelial Cells and Study on Those Characters for Recombinant Virus Infection

2013 ◽  
Vol 40 (4) ◽  
pp. 299-304
Author(s):  
Mi Na Park ◽  
Hyun-Jun Jang ◽  
Dae Ho Keum ◽  
Jin Ae Choi ◽  
Jae Gyu Yoo ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Virus Infection ◽  
Primary Culture ◽  
Recombinant Virus ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial
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