Purification and characterization of chymotrypsin inhibitors from marine turtle egg white

ABSTRACTNatural antimicrobial peptides are present in different compartments (eggshell, egg white, and vitelline membranes) of the hen egg and are expected to be involved in the protection of the embryo during its development and to contribute to the production of pathogen-free eggs. In the present study, we used vitelline membranes from hen (Gallus gallus) eggs as a source of avian β-defensin 11 (AvBD11). A purification scheme using affinity chromatography and reverse-phase chromatography was developed. Purified AvBD11 was analyzed by a combination of mass spectrometry approaches to characterize its primary sequence and structure. A monoisotopic molecular species at [M + H]+of 9,271.56 Da was obtained, and its N- and C-terminal sequences were determined. We also examined posttranslational modifications and identified the presence of 6 internal disulfide bonds. AvBD11 was found to exhibit antimicrobial activity toward both Gram-positive and Gram-negative bacteria.

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Purification and characterization of lysozyme from Chinese Lueyang black-bone Silky fowl egg white

Preparative Biochemistry & Biotechnology ◽

10.1080/10826068.2018.1476887 ◽

2019 ◽

Vol 49 (3) ◽

pp. 215-221 ◽

Cited By ~ 1

Author(s):

Chen Chen ◽

Xinxin Li ◽

Lijuan Yue ◽

Xian Jing ◽

Yiqi Yang ◽

...

Keyword(s):

Egg White ◽

Purification And Characterization

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Purification and Characterization of Urea Transporter by SDS-PAGE from the Paddy Grown in Urea Treated Soil

Progressive Agriculture ◽

10.3329/pa.v18i2.17463 ◽

2013 ◽

Vol 18 (2) ◽

pp. 41-47

Author(s):

MS Haque ◽

MK Hasan

Keyword(s):

Trypsin Inhibitor ◽

Egg White ◽

Urea Transport ◽

Urea Transporter ◽

Purification And Characterization ◽

Treated Soil ◽

Marker Proteins ◽

Sds Page ◽

New Finding

G-50 chromatography of extract of paddy plant induced by urea revealed the major three peaks F-1, F-2 and F-3 against the absorbance at 280 nm with the number of fractions. Chromatogram was also obtained from the control of extract of paddy, however, urea-induced paddy extract showed the higher absorbance than the control showing the higher protein content. F-1 fraction for both groups having the higher molecules are considered to have the urea transporter and are applied to SDS-PAGE for purification and characterization. For identification of urea transporter, a mixture of marker proteins- ?-galactosidase, BSA, egg white albumin, pepsin, trypsin inhibitor and lysozyme were used. Depending on the mobility of the sample, urea-transporter had been identified as a large band having the MW of 50.6 kDa compared to the MW of BSA. For control, SDS-PAGE shows the same band for urea-transporter but the expression was lower than the urea-treated paddy. In SDS-PAGE gel, both bands were homogenous showing the homogeneity of urea-transporter. The results demonstrate that urea application in the soil induces the expression of urea transporter which will be a new finding for the clarification of mechanism of urea transport in plants.DOI: http://dx.doi.org/10.3329/pa.v18i2.17463 Progress. Agric. 18(2): 41 - 47, 2007

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