An improved method for isolating high-quality DNA fromVitis vinifera nuclei

Immunohistochemistry using mouse retinal cryosections is a routine assay used in vision research. However, retinal tissues are fragile, and it is difficult to obtain an ideal retinal cryosection. Here, we developed a modified method for preparing retinal cryosection. Super Glue was applied on the surface of the sclera before the cornea and the lens are removed from either the unfixed or PFA-fixed mouse eyeballs. The new methods largely prevented retinal detachment in mouse retinal cryosections. Immunostaining of retinal cryosections derived from PFA-fixed mouse eyes using rod and cone markers yielded high-quality immunofluorescent images. Immunolabeling of retinal cryosections obtained from unfixed mouse eyes using a cilium-specific marker had improved orientations of photoreceptor connecting cilia. This new method substantially improves the morphology and immunostaining results of fixed and unfixed mouse eyes.

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Supersonic Jet Epitaxy: An Improved Method for Nitride Deposition

MRS Proceedings ◽

10.1557/proc-395-301 ◽

1995 ◽

Vol 395 ◽

Cited By ~ 1

Author(s):

Peter E. Norris ◽

Long D. Zhu ◽

H. Paul Maruska ◽

Wilson HO ◽

Scott Ustin ◽

...

Keyword(s):

Buffer Layer ◽

Microwave Plasma ◽

Supersonic Jet ◽

Plasma Source ◽

Improved Method ◽

High Quality ◽

Carrier Gas ◽

Sapphire Substrates ◽

Supersonic Jet Epitaxy ◽

Gan Crystal

ABSTRACTGaN was grown by supersonic jet epitaxy(SSJE), seeding triethylgallium in helium carrier gas. Activated nitrogen was supplied by a microwave plasma source. Single crystalline GaN films were deposited on the Si-face 6H-SiC and the c-plane sapphire substrates at 600–670°C. A cubic SiC buffer layer was grown onSi(111) at 800°C by SSJE using dichlorosilane, acetylene, and a high quality GaN crystal was grown on this template at 630°C. The materials high quality was proved by hard rectifying characteristics of a diode with an N-GaN/β-SiC/P-Si(111) structure.

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An Improved Method for the Isolation of Total RNA from Avicennia germinans Leaves

Zeitschrift für Naturforschung C ◽

10.1515/znc-2008-1-222 ◽

2008 ◽

Vol 63 (1-2) ◽

pp. 124-126 ◽

Cited By ~ 14

Author(s):

Daniel Gonzalez-Mendoza ◽

Adriana Quiroz Moreno ◽

Omar Zapata-Perez

Keyword(s):

Genomic Dna ◽

Avicennia Germinans ◽

Improved Method ◽

Fresh Weight ◽

High Quality ◽

Plant Materials ◽

Total Rna ◽

Other Substances ◽

Contaminating Substances

Isolation of high-quality RNA of Avicennia germinans L. tissue is difficult due to high levels of phenols and other substances that interfere when using conventional procedures for the isolation. These substances not only decrease the yield but also the quality of RNA is almost poor. We present here a simple RNA protocol and fast methodology that effectively removes these contaminating substances without affecting the yield. The protocol developed is based on the SDS/phenol method with modifications including β-mercaptoethanol to prevent oxidation of phenolic complexes, and phenol/chloroform extraction is introduced to remove proteins, genomic DNA, and secondary metabolites, and co-precipitated polysaccharides. Both A260/A230 and A260/A280 absorbance ratios of isolated RNA were around 2 and the yield was about 0.3 mg g −1 fresh weight. Good-quality total RNA from leaves of Avicennia germinans could be easily isolated within 2 h by this protocol which avoided the limitation of plant materials and could provide total RNA for all kinds of further molecular studies.

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An Improved Method for High Quality Metagenomics DNA Extraction from Human and Environmental Samples

Scientific Reports ◽

10.1038/srep26775 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 79

Author(s):

Satyabrata Bag ◽

Bipasa Saha ◽

Ojasvi Mehta ◽

D. Anbumani ◽

Naveen Kumar ◽

...

Keyword(s):

Dna Extraction ◽

Environmental Samples ◽

Improved Method ◽

High Quality

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Identification of leaf-mining insects via DNA recovered from empty mines

FACETS ◽

10.1139/facets-2016-0026 ◽

2017 ◽

Vol 1 (1) ◽

pp. 217-224 ◽

Cited By ~ 3

Author(s):

Julia J. Mlynarek ◽

Jin-Hong Kim ◽

Stephen B. Heard

Keyword(s):

Community Dynamics ◽

Leaf Miner ◽

Ecological Studies ◽

Improved Method ◽

High Quality ◽

Pest Monitoring ◽

Mtdna Coi ◽

Leaf Mining ◽

Herbivore Community

We report the development of an improved method for the extraction and amplification of leaf miner DNA recovered from empty mines. Our method is simple, easy to use, and foregoes the time-consuming task of scraping out mines required by previous methods. We collected leaves with 1- and 2-day-old vacated mines, cut out and then ground the mined portions, and amplified the mtDNA COI barcode sequence using universal insect primers. We obtained high-quality sequences for 31% of our empty mines: 20% yielded sequences associated with a leaf miner species; and an additional 11% yielded sequences associated with whiteflies, mites, or fungi. Our improved method will facilitate ecological studies determining herbivore community dynamics and agricultural studies for pest monitoring and identification.

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An improved method for isolation of high-quality RNA from starch-rich wheat grains

Journal of Environmental Biology ◽

10.22438/jeb/41/3/mrn-1137 ◽

2020 ◽

Vol 41 (3) ◽

pp. 586-591

Author(s):

M. Goyal ◽

◽

S. Dhillon ◽

P. Kumar ◽

◽

...

Keyword(s):

Improved Method ◽

High Quality ◽

Wheat Grains

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Accurate chromosome-scale haplotype-resolved assembly of human genomes

10.1101/810341 ◽

2019 ◽

Cited By ~ 18

Author(s):

Shilpa Garg ◽

Arkarachai Fungtammasan ◽

Andrew Carroll ◽

Mike Chou ◽

Anthony Schmitt ◽

...

Keyword(s):

Precision Medicine ◽

Population Diversity ◽

Pedigree Information ◽

Structural Variants ◽

Improved Method ◽

High Quality ◽

Haplotype Variation ◽

Human Genomes ◽

Transposon Insertions ◽

Assembly Algorithms

Haplotype-resolved or phased sequence assembly provides a complete picture of genomes and complex genetic variations. However, current phased assembly algorithms either fail to generate chromosome-scale phasing or require pedigree information, which limits their application. We present a method that leverages long accurate reads and long-range conformation data for single individuals to generate chromosome-scale phased assembly within a day. Applied to three public human genomes, PGP1, HG002 and NA12878, our method produced haplotype-resolved assemblies with contig NG50 up to 25 Mb and phased ∼99.5% of heterozygous sites to 98–99% accuracy, outperforming other approaches in terms of both contiguity and phasing completeness. We demonstrate the importance of chromosome-scale phased assemblies to discover structural variants (SVs), including thousands of new transposon insertions, and of highly polymorphic and medically important regions such as HLA and KIR. Our improved method will enable high-quality precision medicine and facilitate new studies of individual haplotype variation and population diversity.

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An Improved Method of the Adaptive Hierarchical Space Partition Simplification Algorithm on the Point-Based Model

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.915-916.1259 ◽

2014 ◽

Vol 915-916 ◽

pp. 1259-1265

Author(s):

Jie Chen ◽

Jun Ting Cheng

Keyword(s):

Point Cloud ◽

Improved Method ◽

High Quality ◽

Quality Models ◽

Space Partition ◽

Geometrical Error ◽

Novel Approach ◽

Geometrical Features ◽

Model Editing ◽

Cloud Applications

The 3D scanning device can capture millions of points with the excellent geometrical precision except for large amount of redundant ones, which could bring some difficulties for the subsequent digital geometrical processing (DGP), so the simplification of the point cloud has become a considerable study in point cloud applications. Given this problem, we propose a novel approach, which could decrease the geometrical error by partitioning the surface into many patches with some similar geometrical features before using the adaptive hierarchical space partition (AHSP) approach, in order to improve the AHSP simplification. We also experiment on three models and do comparative analysis. Fortunately, the results prove that our algorithm can make the anisotropy feature in the surface of the models described explicitly, the geometrical error decreased by 15.8 percent, and the simplification rate kept up with other approaches. In addition, it can provide the high quality models for the 3D digital model editing, such as the geometrical modeling, the point cloud blending.

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