Construction and characterization of a biotin-regulated gene expression system inEscherichia coli

1997 ◽  
Vol 66 (2) ◽  
pp. 147-158
Author(s):  
Yuo-Sheng Chang ◽  
David Shiuan
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Inescherichia Coli ◽  
Gene Expression System ◽  
Regulated Gene Expression

The Development and Characterization of an Exogenous Green-Light-Regulated Gene Expression System in Marine Cyanobacteria

2015 ◽  
Vol 17 (3) ◽  
pp. 245-251 ◽  
Author(s):  
Amr Badary ◽  
Koichi Abe ◽  
Stefano Ferri ◽  
Katsuhiro Kojima ◽  
Koji Sode
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Green Light ◽  
Marine Cyanobacteria ◽  
Gene Expression System ◽  
Regulated Gene Expression

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

scholarly journals A Novel Tightly Regulated Gene Expression System for the Human Intestinal Symbiont Bacteroides thetaiotaomicron

2016 ◽  
Vol 7 ◽  
Author(s):  
Nikki Horn ◽  
Ana L. Carvalho ◽  
Karin Overweg ◽  
Udo Wegmann ◽  
Simon R. Carding ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Bacteroides Thetaiotaomicron ◽  
Gene Expression System ◽  
Regulated Gene Expression

scholarly journals Viral Evolution as a Tool to Improve the Tetracycline-regulated Gene Expression System

2004 ◽  
Vol 279 (18) ◽  
pp. 18776-18782 ◽  
Author(s):  
Atze T. Das ◽  
Xue Zhou ◽  
Monique Vink ◽  
Bep Klaver ◽  
Koen Verhoef ◽  
...  
Keyword(s):  
Gene Expression ◽  
Viral Evolution ◽  
Expression System ◽  
Gene Expression System ◽  
Regulated Gene Expression

A Coumermycin/Novobiocin-Regulated Gene Expression System

2003 ◽  
Vol 14 (17) ◽  
pp. 1619-1629 ◽  
Author(s):  
Hui-Fen Zhao ◽  
Jason Boyd ◽  
Normand Jolicoeur ◽  
Shi-Hsiang Shen
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Gene Expression System ◽  
Regulated Gene Expression

scholarly journals Characterization of the ethanol-inducible alc gene expression system in tomato

2005 ◽  
Vol 56 (416) ◽  
pp. 1635-1642 ◽  
Author(s):  
G. Ali Garoosi ◽  
Michael G. Salter ◽  
Mark X. Caddick ◽  
A. Brian Tomsett
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Gene Expression System

scholarly journals Use of a Stringent Dimerizer-Regulated Gene Expression System for Controlled BMP2 Delivery

2006 ◽  
Vol 14 (5) ◽  
pp. 684-691 ◽  
Author(s):  
Jeong-Tae Koh ◽  
Chunxi Ge ◽  
Ming Zhao ◽  
Zhuo Wang ◽  
Paul H. Krebsbach ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Gene Expression System ◽  
Regulated Gene Expression

Identification and characterization of an essential gene inListeria monocytogenesusing an inducible gene expression system

2011 ◽  
Vol 2 (3) ◽  
pp. 150-159 ◽  
Author(s):  
Kiera M. Considine ◽  
Roy D. Sleator ◽  
Alan L. Kelly ◽  
Gerald F. Fitzgerald ◽  
Colin Hill
Keyword(s):  
Gene Expression ◽  
Essential Gene ◽  
Expression System ◽  
Inducible Gene Expression ◽  
Gene Expression System ◽  
Identification And Characterization ◽  
Inducible Gene

ChemInform Abstract: Development of Recombinant Adenovirus Carrying MicroRNA-Regulated Gene Expression System

ChemInform ◽  
2011 ◽  
Vol 42 (12) ◽  
pp. no-no
Author(s):  
Fuminori Sakurai ◽  
Hiroyuki Mizuguchi
Keyword(s):  
Gene Expression ◽  
Recombinant Adenovirus ◽  
Expression System ◽  
Gene Expression System ◽  
Regulated Gene Expression
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