Attenuation of nitric oxide synthase gene expression in rat lung induced by hypoxia

1996 ◽  
Vol 16 (3) ◽  
pp. 148-151
Author(s):  
Dai Aiguo ◽  
Zhahg Zhenxiang ◽  
Niu Ruji ◽  
Xu Yongjian ◽  
Duan Shengfu
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Lung ◽  
Oxide Synthase

Nitric oxide synthase type I and type III gene expression are developmentally regulated in rat lung

1994 ◽  
Vol 266 (6) ◽  
pp. L635-L641 ◽  
Author(s):  
A. J. North ◽  
R. A. Star ◽  
T. S. Brannon ◽  
K. Ujiie ◽  
L. B. Wells ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Fetal Lung ◽  
Mrna Abundance ◽  
Type I ◽  
Fetal Life ◽  
Rat Lung ◽  
Developmentally Regulated ◽  
Oxide Synthase

The successful transition from fetal to neonatal life involves a marked decline in pulmonary vascular resistance which is modulated in part by endothelium-derived nitric oxide. To define the molecular processes which prepare the pulmonary circulation for nitric oxide mediation of vasodilatation at the time of birth, we determined the ontogeny of endothelial nitric oxide synthase (NOS-III) gene expression in lungs from fetal and newborn rats. Maturational changes in lung neuronal NOS (NOS-I) expression were also investigated; the latter isoform has been localized to rat bronchiolar epithelium. NOS proteins were examined by immunoblot analysis, and mRNA abundance was assessed in reverse transcription-polymerase chain reaction assays. Both NOS-III and NOS-I protein were detectable in 16-day fetal lung, they increased 3.8- and 3.1-fold, respectively, to maximal levels at 20 days of gestation (term = 22 day), and they fell postnatally (1-5 days). In parallel with the findings for NOS-III protein, NOS-III mRNA increased from 16 to 20 days gestation and fell after birth. In contrast, NOS-I mRNA abundance declined during late fetal life and rose postnatally. These findings were confirmed by Northern analyses. Thus NOS-III and NOS-I gene expression are developmentally regulated in rat lung, with maximal NOS-III and NOS-I protein present near term. The regulation of pulmonary NOS-III may primarily involve alterations in transcription or mRNA stability, whereas NOS-I expression in the maturing lung may also be mediated by additional posttranscriptional processes.

Prolonged in vivo hypoxia enhances nitric oxide synthase type I and type III gene expression in adult rat lung.

1995 ◽  
Vol 13 (2) ◽  
pp. 167-174 ◽  
Author(s):  
P W Shaul ◽  
A J North ◽  
T S Brannon ◽  
K Ujiie ◽  
L B Wells ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Type I ◽  
Rat Lung ◽  
Type Iii ◽  
Adult Rat ◽  
Oxide Synthase

scholarly journals Protein and gene expression of nitric oxide synthase isoforms I and III in the rat penile shaft

2002 ◽  
Vol 14 (4) ◽  
pp. 316-316
Author(s):  
AL Burnett ◽  
C M Gonzalez ◽  
R E Brannigan ◽  
T Bervig ◽  
D Zelner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Penile Shaft ◽  
Oxide Synthase

Increased vascular endothelin-1 gene expression with unaltered nitric oxide synthase levels in fructose-induced hypertensive rats

Metabolism ◽  
2001 ◽  
Vol 50 (1) ◽  
pp. 74-78 ◽  
Author(s):  
Dae Ho Lee ◽  
JongUn Lee ◽  
Dae Gill Kang ◽  
Yun Woong Paek ◽  
Dong Jin Chung ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Hypertensive Rats ◽  
Endothelin 1 ◽  
Oxide Synthase
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