Effects of pertussis and cholera toxin on the interferon-γ stimulated immunocytochemical staining of ICAM-1 and inositol phosphate formation in a human renal carcinoma cell line

1993 ◽  
Vol 64 (1) ◽  
pp. 345-350 ◽  
Author(s):  
Alastair B. Hansen ◽  
Pierre N. Bouchelouche ◽  
Claus B. Andersen
Blood ◽  
1987 ◽  
Vol 69 (4) ◽  
pp. 1053-1057 ◽  
Author(s):  
JB Sherwood ◽  
ER Burns ◽  
D Shouval

Abstract We used our recently reported stable, transformed human renal carcinoma cell line as a model system to study the role of 3′,5′-adenosine monophosphate (cAMP) in erythropoietin secretion. The erythropoietin produced by these cells is both biologically active and immunologically cross-reactive with purified native human hormone in our radioimmunoassay. Erythropoietin release by these renal carcinoma cells appears to be stimulated by cAMP as well as by the phosphodiesterase inhibitor 3-isobutyl-1-methyl-xanthine (MIX). The response to cAMP involves a rapid and enhanced release of hormone, which occurred within 30 minutes of exposure of the cells to the effector and continued for at least 4 hours. Intracellular erythropoietin was higher in the control cultures than in the cells treated with cAMP, suggesting that cAMP stimulates the release of a storage pool of hormone. The ability of cAMP and MIX to elicit the release of erythropoietin suggests that a cAMP-mediated mechanism is involved in the release of this hormone.


2016 ◽  
Vol 49 (2) ◽  
pp. 457-470 ◽  
Author(s):  
Clelia Prattichizzo ◽  
Margherita Gigante ◽  
Paola Pontrelli ◽  
Alessandro Stella ◽  
Maria Teresa Rocchetti ◽  
...  

2006 ◽  
Vol 97 (4) ◽  
pp. 296-304 ◽  
Author(s):  
S. Fatemeh Moosavi-Nejad ◽  
S. Hamid R. Hosseini ◽  
Makoto Satoh ◽  
Kazuyoshi Takayama

Tumor Biology ◽  
2014 ◽  
Vol 36 (2) ◽  
pp. 595-604 ◽  
Author(s):  
Antonio Hernandes Chaves Neto ◽  
Karin Juliane Pelizzaro-Rocha ◽  
Maruska Neufert Fernandes ◽  
Carmen Veríssima Ferreira-Halder

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