Antiphage activity of some actinomycetes

Recent studies on preservation property in the field of materials science suggest that a newly synthesized material can retain the biological properties of the raw material. Still, further study is...

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Antiphage activity of natural and synthetic substances: a new age for antivirals?

Future Microbiology ◽

10.2217/fmb-2019-0320 ◽

2020 ◽

Vol 15 (9) ◽

pp. 767-777

Author(s):

Jhonatan M Ribeiro ◽

Giovana N Pereira ◽

Renata KT Kobayashi ◽

Gerson Nakazato

Keyword(s):

Mammalian Cells ◽

Eukaryotic Cell ◽

Future Perspective ◽

Viral Diseases ◽

Obligate Intracellular ◽

Intracellular Parasites ◽

A Genome ◽

Living Organisms ◽

Biological Entities ◽

Antiphage Activity

Viruses are considered biological entities that possess a genome and can adapt to the environment of living organisms. Since they are obligate intracellular parasites, their cycle of replication can result in cell death, and consequently, some viruses are harmful to mammalian cells and can cause disease in humans. Therefore, the search for substances for the treatment of viral diseases can be accomplished through the use of bacteriophages as models for eukaryotic cell viruses. Thus, this review highlights the main studies identifying substances with antiphage activity in comparison assays involving phages and eukaryotic viruses, in order to explore the potential of these substances as antivirals. As a future perspective, this approach may help at the beginning of an Antiviral Age.

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ChemInform Abstract: Antiphage Activity of Hexachloroplatinates of Substituted Thioniabicycloalkanes (I).

ChemInform ◽

10.1002/chin.200152133 ◽

2010 ◽

Vol 32 (52) ◽

pp. no-no

Author(s):

S. K. Klimenko ◽

T. V. Stolbova ◽

L. K. Kulikova ◽

F. M. Shub

Keyword(s):

Antiphage Activity

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ChemInform Abstract: Synthesis and Antiphage Activity of Substituted N-Arylcyclohexenylamines (I).

ChemInform ◽

10.1002/chin.200201095 ◽

2010 ◽

Vol 33 (1) ◽

pp. no-no

Author(s):

V. V. Sorokin ◽

A. P. Kriven'ko ◽

N. A. Vinogradova ◽

O. P. Plotnikov

Keyword(s):

Antiphage Activity

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Mutational Analysis of the Antitoxin in the Lactococcal Type III Toxin-Antitoxin System AbiQ

Applied and Environmental Microbiology ◽

10.1128/aem.00572-15 ◽

2015 ◽

Vol 81 (11) ◽

pp. 3848-3855 ◽

Cited By ~ 6

Author(s):

Maxime Bélanger ◽

Sylvain Moineau

Keyword(s):

Noncoding Rna ◽

Mutational Analysis ◽

Point Mutations ◽

Northern Hybridization ◽

Type Iii ◽

Infection Mechanism ◽

Digestion Profile ◽

Rapid Amplification ◽

Antiphage Activity

ABSTRACTThe lactococcal abortive phage infection mechanism AbiQ recently was classified as a type III toxin-antitoxin system in which the toxic protein (ABIQ) is regulated following cleavage of its repeated noncoding RNA antitoxin (antiQ). In this study, we investigated the role of the antitoxin in antiphage activity. The cleavage ofantiQby ABIQ was characterized using 5′ rapid amplification of cDNA ends PCR and was located in an adenine-rich region ofantiQ. We next generated a series of derivatives with point mutations withinantiQor with various numbers ofantiQrepetitions. These modifications were analyzed for their effect on the antiphage activity (efficiency of plaquing) and on the endoribonuclease activity (Northern hybridization). We observed that increasing or reducing the number ofantiQrepeats significantly decreased the antiphage activity of the system. Several point mutations had a similar effect on the antiphage activity and were associated with changes in the digestion profile ofantiQ. Interestingly, a point mutation in the putative pseudoknot structure ofantiQmutants led to an increased AbiQ antiphage activity, thereby offering a novel way to increase the activity of an abortive infection mechanism.

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New methods of production and partial purification of an antiviral substance from Penicillium cyaneo-fulvum

Canadian Journal of Microbiology ◽

10.1139/m68-033 ◽

1968 ◽

Vol 14 (3) ◽

pp. 189-196 ◽

Cited By ~ 1

Author(s):

Tam S. David-West ◽

Patricia M. Cooke ◽

J. W. Stevenson

Keyword(s):

Influenza A ◽

Enzyme Inactivation ◽

Partial Purification ◽

Active Principle ◽

Ultraviolet Absorption Spectrum ◽

Freezing And Thawing ◽

New Methods ◽

Repeated Freezing ◽

Antiviral Substance ◽

Antiphage Activity

A strain of Penicillium cyaneo-fulvum isolated in this laboratory elaborates an antiviral substance in Czapek-Dox broth which inhibits the multiplication of influenza A and B and Newcastle disease viruses in modified Maitland tissue cultures. However, the multiplication of these viruses as well as mumps virus was not inhibited when tested in embryonated hens' eggs. No antiphage activity was demonstrated against a ribonucleic acid containing phage (f2) and a single-stranded deoxyribonucleic acid containing phage [Formula: see text]. The antiviral substance in a semipurified form, derived by precipitation of impurities from the culture filtrates with acetone, contains sugars, amino acids, and nucleic acid components, and shows a peak at about 270 mμ in its ultraviolet absorption spectrum, with a 280 mμ/260 mμ ratio of 0.97. It is stable to heat, lyophilization, repeated freezing and thawing and it is not dialyzable. Results of enzyme inactivation of the active principle were not conclusive.

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