Polycyclic aromatic hydrocarbons in crude oil-contaminated soil: A two-step method for the isolation and characterization of PAHs

1997 ◽  
Vol 4 (4) ◽  
pp. 217-222 ◽  
Author(s):  
Mohammed Shahid Akhlaq
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Crude Oil ◽  
Aromatic Hydrocarbons ◽  
Contaminated Soil ◽  
Step Method ◽  
Isolation And Characterization ◽  
Polycyclic Aromatic

scholarly journals Stenotrophomonas sp. Pemsol isolated from crude oil contaminated soil in Mexico that can degrade polycyclic aromatic hydrocarbons and its whole genome sequence analyzed

2019 ◽  
Author(s):  
Temidayo O Elufisan ◽  
Isabel C Rodríguez-Luna ◽  
Omotayo O Oyedara ◽  
Alejandro Sánchez-Varela ◽  
Armando Hernandez Mendoza ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Crude Oil ◽  
Aromatic Hydrocarbons ◽  
Genome Analysis ◽  
Contaminated Soil ◽  
Antimicrobial Agents ◽  
Whole Genome ◽  
Chromatography Mass Spectrometry ◽  
Polycyclic Aromatic

Background: Stenotrophomonas are ubiquitous gram-negative bacteria which survive in a wide range of environments. They can use many substances for their growth and are known to be intrinsically resistant to many antimicrobial agents. They have been tested for biotechnological applications, bioremediation and antimicrobial agents because of their recalcitrant nature to many toxic compounds. Method. Stenotrophomonas sp. Pemsol was isolated from a crude oil contaminated soil. The capability of this isolate to tolerate and degrade polycyclic aromatic hydrocarbons (PAHs) (anthracene, anthraquinone, biphenyl, naphthalene, phenanthrene, phenanthridine and xylene) was evaluated on Bush Nell Hass medium containing PAHs as the unique carbon sources. The metabolites formed after 30-day degradation of naphthalene by Pemsol were analyzed using Fourier Transform Infra-red Spectroscopic (FTIR), Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS). Results. Complete degradation of naphthalene at a concentration of 1 mg/mL was obtained and a newly formed catechol peak obtained from the UPLC-MS and GC-MS confirmed the degradation. The strain Pemsol lacked the ability to produce biosurfactant so that it cannot bio-emulsify PAHs. The whole genome analysis of Stenotrophomonas sp. Pemsol revealed a wealth of genes for hydrocarbon utilization and interaction with the environment and the presence of 147 genes associated with the degradation of PAHs, some of which are strain-specific on the genomic islands. Few genes are associated with bio-emulsification indicated that Pemsol without biosurfactant production has a genetic basis. This is the first report of the complete genome analysis sequence of a PAH-degrading Stenotrophomonas. Stenotrophomonas sp. Pemsol possesses features that makes it a good bacterium for genetic engineering and will therefore be a good tool for the remediation of crude oil or PAH-contaminated soil.

scholarly journals Isolation and characterization of a bacterial strain Hydrogenophaga sp. PYR1 for anaerobic pyrene and benzo[a]pyrene biodegradation

RSC Advances ◽  
2017 ◽  
Vol 7 (74) ◽  
pp. 46690-46698 ◽  
Author(s):  
Zaisheng Yan ◽  
Yu Zhang ◽  
Huifang Wu ◽  
Mingzhong Yang ◽  
Haichen Zhang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
River Sediments ◽  
Anaerobic Conditions ◽  
Isolation And Characterization ◽  
Polycyclic Aromatic ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

A pyrene-degrading strainHydrogenophagasp. PYR1 was isolated from PAH-contaminated river sediments and found to be able to degrade high molecular weight-polycyclic aromatic hydrocarbons under both aerobic and anaerobic conditions.

Isolation and characterization of a newly isolated polycyclic aromatic hydrocarbons-degrading Janibacter anophelis strain JY11

2009 ◽  
Vol 172 (2-3) ◽  
pp. 580-586 ◽  
Author(s):  
Guo-Ying Zhang ◽  
Jian-Ya Ling ◽  
Hai-Bo Sun ◽  
Jie Luo ◽  
Yuan-Yuan Fan ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Isolation And Characterization ◽  
Polycyclic Aromatic

scholarly journals Stenotrophomonas sp. Pemsol isolated from crude oil contaminated soil in Mexico that can degrade polycyclic aromatic hydrocarbons and its whole genome sequence analyzed

2019 ◽  
Author(s):  
Temidayo O Elufisan ◽  
Isabel C Rodríguez-Luna ◽  
Omotayo O Oyedara ◽  
Alejandro Sánchez-Varela ◽  
Armando Hernandez Mendoza ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Crude Oil ◽  
Aromatic Hydrocarbons ◽  
Genome Analysis ◽  
Contaminated Soil ◽  
Antimicrobial Agents ◽  
Whole Genome ◽  
Chromatography Mass Spectrometry ◽  
Polycyclic Aromatic

Background: Stenotrophomonas are ubiquitous gram-negative bacteria which survive in a wide range of environments. They can use many substances for their growth and are known to be intrinsically resistant to many antimicrobial agents. They have been tested for biotechnological applications, bioremediation and antimicrobial agents because of their recalcitrant nature to many toxic compounds. Method. Stenotrophomonas sp. Pemsol was isolated from a crude oil contaminated soil. The capability of this isolate to tolerate and degrade polycyclic aromatic hydrocarbons (PAHs) (anthracene, anthraquinone, biphenyl, naphthalene, phenanthrene, phenanthridine and xylene) was evaluated on Bush Nell Hass medium containing PAHs as the unique carbon sources. The metabolites formed after 30-day degradation of naphthalene by Pemsol were analyzed using Fourier Transform Infra-red Spectroscopic (FTIR), Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS). Results. Complete degradation of naphthalene at a concentration of 1 mg/mL was obtained and a newly formed catechol peak obtained from the UPLC-MS and GC-MS confirmed the degradation. The strain Pemsol lacked the ability to produce biosurfactant so that it cannot bio-emulsify PAHs. The whole genome analysis of Stenotrophomonas sp. Pemsol revealed a wealth of genes for hydrocarbon utilization and interaction with the environment and the presence of 147 genes associated with the degradation of PAHs, some of which are strain-specific on the genomic islands. Few genes are associated with bio-emulsification indicated that Pemsol without biosurfactant production has a genetic basis. This is the first report of the complete genome analysis sequence of a PAH-degrading Stenotrophomonas. Stenotrophomonas sp. Pemsol possesses features that makes it a good bacterium for genetic engineering and will therefore be a good tool for the remediation of crude oil or PAH-contaminated soil.

Phytoremediation of Alkylated Polycyclic Aromatic Hydrocarbons in a Crude Oil-Contaminated Soil

2006 ◽  
Vol 169 (1-4) ◽  
pp. 207-220 ◽  
Author(s):  
Paul M. White ◽  
Duane C. Wolf ◽  
Gregory J. Thoma ◽  
Charles M. Reynolds
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Crude Oil ◽  
Aromatic Hydrocarbons ◽  
Contaminated Soil ◽  
Polycyclic Aromatic ◽  
Alkylated Polycyclic Aromatic Hydrocarbons
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